C57BL/6JCya-Klf15em1flox/Cya
Common Name:
Klf15-flox
Product ID:
S-CKO-12989
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Klf15-flox
Strain ID
CKOCMP-66277-Klf15-B6J-VA
Gene Name
Product ID
S-CKO-12989
Gene Alias
1810013I09Rik; CKLF; KKLF; hlb444
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Klf15em1flox/Cya mice (Catalog S-CKO-12989) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032174
NCBI RefSeq
NM_023184
Target Region
Exon 2
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Klf15, also known as Krüppel-like factor 15, is a transcription factor with diverse biological functions. It is involved in multiple pathways, such as those related to metabolism, inflammation, and cell-phenotype regulation, playing an overall crucial role in maintaining normal physiological functions and in disease-related processes. Genetic models, like gene knockout (KO) and conditional knockout (CKO) mouse models, are valuable tools for studying Klf15's functions.
In immobility-induced muscle atrophy, mice with skeletal muscle KLF15 deficiency are protected from atrophy, suggesting Klf15 is part of the Piezo1/KLF15/IL-6 axis mediating this atrophy [1]. In cardiac ischemic injury, WWP1 targets KLF15 for polyubiquitination and degradation, exacerbating cardiomyocyte inflammation; inhibition of WWP1 mitigates this [2]. In pancreatic cancer, KLF15 suppresses cancer stem cell stemness by promoting Nanog degradation [3]. In hypertensive renal injury, SIRT7 mitigates renal ferroptosis, fibrosis, and injury by facilitating the KLF15/Nrf2 signaling [4]. In the heart, KLF15 is an inhibitor of pathological cardiac hypertrophy and fibrosis [5]. In liver, KLF15-Cyp3a11 axis regulates rifampicin-induced liver injury; KLF15 knockout attenuates hepatotoxicity of rifampicin [6]. In thoracic aortic dissection, Klf15KO mice are more susceptible to TAD, and KLF15 maintains VSMC contractile phenotype by interacting with MRTFB [7]. In cardiomyocytes, KLF15 deficiency leads to increased oxidative stress due to reduced NAMPT and NAD + levels [8]. In triple-negative breast cancer, exogenous KLF15 suppresses cell growth and metastasis by downregulating CCL2 and CCL7 [9].
In conclusion, Klf15 is involved in a wide range of biological processes including muscle atrophy, cardiac function, cancer stemness, renal injury, and more. The use of Klf15 KO/CKO mouse models has significantly contributed to understanding its role in diseases such as muscle atrophy, cardiac ischemic injury, pancreatic cancer, hypertensive renal injury, and others, providing potential therapeutic targets for these conditions.
References:
1. Hirata, Yu, Nomura, Kazuhiro, Kato, Daisuke, Wake, Hiroaki, Ogawa, Wataru. 2022. A Piezo1/KLF15/IL-6 axis mediates immobilization-induced muscle atrophy. In The Journal of clinical investigation, 132, 1-13. doi:10.1172/JCI154611. https://pubmed.ncbi.nlm.nih.gov/35290243/
2. Lu, Xia, Yang, Boshen, Qi, Ruiqiang, Wang, Yan, Song, Juan. 2023. Targeting WWP1 ameliorates cardiac ischemic injury by suppressing KLF15-ubiquitination mediated myocardial inflammation. In Theranostics, 13, 417-437. doi:10.7150/thno.77694. https://pubmed.ncbi.nlm.nih.gov/36593958/
3. Jiang, Wenna, Liu, Lin, Wang, Meng, Liu, Jing, Ren, Li. 2024. KLF15 suppresses stemness of pancreatic cancer by decreasing USP21-mediated Nanog stability. In Cellular and molecular life sciences : CMLS, 81, 417. doi:10.1007/s00018-024-05442-6. https://pubmed.ncbi.nlm.nih.gov/39367978/
4. Li, Xue-Ting, Song, Jia-Wei, Zhang, Zhen-Zhou, Liu, Xiao-Yan, Zhong, Jiu-Chang. 2022. Sirtuin 7 mitigates renal ferroptosis, fibrosis and injury in hypertensive mice by facilitating the KLF15/Nrf2 signaling. In Free radical biology & medicine, 193, 459-473. doi:10.1016/j.freeradbiomed.2022.10.320. https://pubmed.ncbi.nlm.nih.gov/36334846/
5. Zhao, Yuguang, Song, Wenjing, Wang, Lizhe, Han, Fujun, Cai, Lu. 2019. Multiple roles of KLF15 in the heart: Underlying mechanisms and therapeutic implications. In Journal of molecular and cellular cardiology, 129, 193-196. doi:10.1016/j.yjmcc.2019.01.024. https://pubmed.ncbi.nlm.nih.gov/30831134/
6. Hou, Wanqing, Huo, Ku-Geng, Guo, Xiaohua, Ma, Zhenghai, Han, Shuxin. 2024. KLF15-Cyp3a11 Axis Regulates Rifampicin-Induced Liver Injury. In Drug metabolism and disposition: the biological fate of chemicals, 52, 606-613. doi:10.1124/dmd.123.001617. https://pubmed.ncbi.nlm.nih.gov/38670799/
7. Fang, Guangming, Tian, Yexuan, Huang, Shan, Du, Jie, Gao, Shijuan. 2024. KLF15 maintains contractile phenotype of vascular smooth muscle cells and prevents thoracic aortic dissection by interacting with MRTFB. In The Journal of biological chemistry, 300, 107260. doi:10.1016/j.jbc.2024.107260. https://pubmed.ncbi.nlm.nih.gov/38582447/
8. Li, Le, Xu, Weiyi, Zhang, Lilei. 2021. KLF15 Regulates Oxidative Stress Response in Cardiomyocytes through NAD. In Metabolites, 11, . doi:10.3390/metabo11090620. https://pubmed.ncbi.nlm.nih.gov/34564436/
9. Kanyomse, Quist, Le, Xin, Tang, Jun, Zeng, Xiaohua, Xiang, Tingxiu. 2022. KLF15 suppresses tumor growth and metastasis in Triple-Negative Breast Cancer by downregulating CCL2 and CCL7. In Scientific reports, 12, 19026. doi:10.1038/s41598-022-23750-4. https://pubmed.ncbi.nlm.nih.gov/36347994/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen