C57BL/6JCya-Slnem1flox/Cya
Common Name:
Sln-flox
Product ID:
S-CKO-13062
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Sln-flox
Strain ID
CKOCMP-66402-Sln-B6J-VA
Gene Name
Product ID
S-CKO-13062
Gene Alias
2310045A07Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slnem1flox/Cya mice (Catalog S-CKO-13062) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000048485
NCBI RefSeq
NM_025540
Target Region
Exon 2
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Sln, also known as sarcolipin, is a protein-related gene. It uncouples sarco -/endoplasmic reticulum Ca2+-ATPase (SERCA), increasing ATP consumption and contributing to heat liberation, thus playing a role in maintaining metabolic homeostasis in human skeletal muscle [1]. This thermogenic mechanism in skeletal muscle may offer a strategy against obesity and related metabolic disorders.
In a study on human skeletal muscle cells with stable Sln knockdown (SLN-KD), glucose and oleic acid (OA) oxidation were diminished, basal respiration was reduced, and the gene expression levels of peroxisome proliferator-activated receptor γ coactivator 1α (PGC1α) and forkhead box O1 (FOXO1) were decreased. Additionally, there was increased lipid formation and accumulation in SLN-KD cells [1].
In conclusion, Sln is crucial for metabolic homeostasis in human skeletal muscle. Findings from Sln-KD cell models suggest that therapeutic strategies involving Sln-mediated futile cycling of SERCA could have significant implications for treating obesity and associated metabolic disorders.
References：
1. Mengeste, Abel M, Katare, Parmeshwar, Dalmao Fernandez, Andrea, Thoresen, G Hege, Kase, Eili Tranheim. 2022. Knockdown of sarcolipin (SLN) impairs substrate utilization in human skeletal muscle cells. In Molecular biology reports, 49, 6005-6017. doi:10.1007/s11033-022-07387-0. https://pubmed.ncbi.nlm.nih.gov/35364719/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen