C57BL/6NCya-Dda1em1flox/Cya
Common Name:
Dda1-flox
Product ID:
S-CKO-13120
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dda1-flox
Strain ID
CKOCMP-66498-Dda1-B6N-VA
Gene Name
Product ID
S-CKO-13120
Gene Alias
1500034J01Rik; 1700095J19Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Dda1em1flox/Cya mice (Catalog S-CKO-13120) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000124745
NCBI RefSeq
NM_025600
Target Region
Exon 2~5
Size of Effective Region
~4.3 kb
Detailed Document
Overview of Gene Research
DDA1, also known as DET1 and DDB1 associated 1, is an evolutionarily conserved gene. It is involved in multiple important biological functions. It has been linked to the ubiquitin-proteasome pathway, and is an integral component of the CRL4CSA ubiquitin ligase complex, regulating transcription-coupled nucleotide excision repair (TC-NER) dynamics [2,3]. It is also associated with several signaling pathways like STAT3, NFκB/CSN2/GSK-3β, and may play a role in cell cycle regulation [1,4].
In breast cancer, DDA1 and p-STAT3 levels are elevated in cisplatin-resistant cells. DHA treatment represses cell proliferation and induces apoptosis by suppressing STAT3 phosphorylation, and DDA1 knockdown inhibits cyclin expression, promotes cell cycle arrest, and induces apoptosis of resistant cells [1]. In stage IIB-IIC colon cancer, DDA1 promotes tumor progression by activating the NFκB/CSN2/GSK-3β signaling pathway, and its overexpression in colon cancer lines promotes cell proliferation, cell cycle progression, and invasion [4]. In lung cancer, DDA1 overexpression promotes proliferation of lung tumour cells and tumour progression in vivo, potentially through promoting cyclins and cell cycle progression [5].
In conclusion, DDA1 plays crucial roles in various biological processes such as DNA repair, cell cycle regulation, and in multiple cancer-related disease conditions like breast, colon, and lung cancers. Understanding DDA1's functions through studies, including potential gene knockout models, could provide new insights into cancer development mechanisms and potential therapeutic targets.
References:
1. Zhang, Jing, Li, Yang, Wang, Ji-Guo, Huang, Guo-Dong, Luo, Chang-Guo. 2023. Dihydroartemisinin Affects STAT3/DDA1 Signaling Pathway and Reverses Breast Cancer Resistance to Cisplatin. In The American journal of Chinese medicine, 51, 445-459. doi:10.1142/S0192415X23500234. https://pubmed.ncbi.nlm.nih.gov/36891981/
2. Llerena Schiffmacher, Diana A, Lee, Shun-Hsiao, Kliza, Katarzyna W, Vermeulen, Wim, Pines, Alex. 2024. The small CRL4CSA ubiquitin ligase component DDA1 regulates transcription-coupled repair dynamics. In Nature communications, 15, 6374. doi:10.1038/s41467-024-50584-7. https://pubmed.ncbi.nlm.nih.gov/39075067/
3. Schiffmacher, Diana Llerena, Lee, Shun-Hsiao, Kliza, Katarzyna W, Vermeulen, Wim, Pines, Alex. 2023. DDA1, a novel factor in transcription-coupled repair, modulates CRL4CSA dynamics at DNA damage-stalled RNA polymerase II. In Research square, , . doi:10.21203/rs.3.rs-3385435/v1. https://pubmed.ncbi.nlm.nih.gov/37886519/
4. Zhao, Senlin, Tang, Huamei, Yan, Dongwang, Qin, Xuebin, Peng, Zhihai. . DDA1 promotes stage IIB-IIC colon cancer progression by activating NFκB/CSN2/GSK-3β signaling. In Oncotarget, 7, 19794-812. doi:10.18632/oncotarget.7847. https://pubmed.ncbi.nlm.nih.gov/26942699/
5. Cheng, Lin, Yang, Qianmei, Li, Can, Wei, Yuquan, Deng, Hongxin. 2017. DDA1, a novel oncogene, promotes lung cancer progression through regulation of cell cycle. In Journal of cellular and molecular medicine, 21, 1532-1544. doi:10.1111/jcmm.13084. https://pubmed.ncbi.nlm.nih.gov/28211159/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen