C57BL/6JCya-Chtopem1flox/Cya
Common Name
Chtop-flox
Product ID
S-CKO-13130
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-66511-Chtop-B6J-VA
When using this mouse strain in a publication, please cite “Chtop-flox Mouse (Catalog S-CKO-13130) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Chtop-flox
Strain ID
CKOCMP-66511-Chtop-B6J-VA
Gene Name
Product ID
S-CKO-13130
Gene Alias
2500003M10Rik, Fop, Srag
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 3
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000049937
NCBI RefSeq
NM_001293776.1
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Overview of Gene Research
Chtop, short for Chromatin target of protein arginine methyltransferase, is a versatile regulator involved in gene-specific transcription and mRNA export. It binds competitively to arginine methyltransferases PRMT1 and PRMT5, promoting asymmetric or symmetric methylation of arginine residues respectively. Chtop is also a component of the TREX complex, which links transcription elongation to mRNA export [1,4].
Knocking down Chtop in chemoresistant epithelial ovarian cancer cells significantly enhanced cisplatin-induced apoptosis, reduced cell stemness, and decreased metastatic potential, suggesting it might be a promising target to overcome chemoresistance [2]. In microglia, knocking down Chtop decreased proinflammatory cytokine expression and altered cell metabolism, alleviating microglia-mediated neuroinflammation in a mouse model [3].
In conclusion, Chtop plays crucial roles in various biological processes such as gene expression regulation, mRNA export, and is associated with diseases like chemoresistant epithelial ovarian cancer and microglia-mediated neuroinflammation. Studies with Chtop knockdown models have provided insights into its functions in these disease-related processes, highlighting its potential as a therapeutic target.
References：
1. Izumikawa, Keiichi, Ishikawa, Hideaki, Simpson, Richard J, Takahashi, Nobuhiro. 2018. Modulating the expression of Chtop, a versatile regulator of gene-specific transcription and mRNA export. In RNA biology, 15, 849-855. doi:10.1080/15476286.2018.1465795. https://pubmed.ncbi.nlm.nih.gov/29683372/
2. Feng, Xiaojie, Bai, Xupeng, Ni, Jie, Graham, Peter, Li, Yong. 2019. CHTOP in Chemoresistant Epithelial Ovarian Cancer: A Novel and Potential Therapeutic Target. In Frontiers in oncology, 9, 557. doi:10.3389/fonc.2019.00557. https://pubmed.ncbi.nlm.nih.gov/31380263/
3. Zhou, Xin, Lv, Mengfei, Duan, Zhongying, Liu, Jiake, Cui, Yu. . CHTOP Promotes Microglia-Mediated Inflammation by Regulating Cell Metabolism and Inflammatory Gene Expression. In Journal of immunology (Baltimore, Md. : 1950), 212, 677-688. doi:10.4049/jimmunol.2300572. https://pubmed.ncbi.nlm.nih.gov/38117276/
4. Chang, Chung-Te, Hautbergue, Guillaume M, Walsh, Matthew J, Philipsen, Sjaak, Wilson, Stuart A. 2013. Chtop is a component of the dynamic TREX mRNA export complex. In The EMBO journal, 32, 473-86. doi:10.1038/emboj.2012.342. https://pubmed.ncbi.nlm.nih.gov/23299939/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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