C57BL/6JCya-Rps19bp1em1flox/Cya
Common Name:
Rps19bp1-flox
Product ID:
S-CKO-13150
Background:
C57BL/6JCya
Product Type
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Genotype
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Basic Information
Strain Name
Rps19bp1-flox
Strain ID
CKOCMP-66538-Rps19bp1-B6J-VA
Gene Name
Product ID
S-CKO-13150
Gene Alias
2510038A11Rik; S19BP
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rps19bp1em1flox/Cya mice (Catalog S-CKO-13150) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000052499
NCBI RefSeq
NM_175109
Target Region
Exon 3~4
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Rps19bp1, whose full name is ribosomal protein S19 binding protein 1, is involved in multiple biological processes. It plays a role in ribosome biogenesis, specifically in 40 S ribosomal subunit assembly, which is crucial for coordinating ribosome biogenesis and promoting erythropoiesis [1]. It is also part of regulatory axes in diseases, like the LINC00106/RPS19BP1/p53 axis in prostate cancer, and is associated with protein-folding processes in pancreatic β-cells in type 2 diabetes [2,3].
In a study using conditional knockout mouse models, depletion of Atf4 in hematopoietic cells led to erythroid differentiation defects and hypoplastic anemia. Mechanistically, ATF4 directly regulates Rps19bp1 transcription, and Rps19bp1 is essential for ribosome biogenesis in erythropoiesis. When Atf4 was depleted under 5-fluorouracil-induced stress, the recovery of hematopoietic lineages was impeded, highlighting the importance of the ATF4-RPS19BP1 axis in this process [1].
In conclusion, Rps19bp1 is essential for ribosome biogenesis and erythropoiesis, as demonstrated by gene-knockout-based research in mouse models. In disease contexts, its role in the LINC00106/RPS19BP1/p53 axis in prostate cancer and its association with protein-folding in pancreatic β-cells in type 2 diabetes suggest its potential as a therapeutic target in these diseases.
References：
1. Zheng, Zhaofeng, Yang, Shangda, Gou, Fanglin, Cheng, Hui, Cheng, Tao. . The ATF4-RPS19BP1 axis modulates ribosome biogenesis to promote erythropoiesis. In Blood, 144, 742-756. doi:10.1182/blood.2023021901. https://pubmed.ncbi.nlm.nih.gov/38657191/
2. Lu, Lingxiang, Tian, Zhen, Lu, Jicheng, Guo, Shuai, Huang, Yuhua. 2023. LINC00106/RPS19BP1/p53 axis promotes the proliferation and migration of human prostate cancer cells. In PeerJ, 11, e15232. doi:10.7717/peerj.15232. https://pubmed.ncbi.nlm.nih.gov/37180577/
3. Klyosova, Elena, Azarova, Iuliia, Buikin, Stepan, Polonikov, Alexey. 2023. Differentially Expressed Genes Regulating Glutathione Metabolism, Protein-Folding, and Unfolded Protein Response in Pancreatic β-Cells in Type 2 Diabetes Mellitus. In International journal of molecular sciences, 24, . doi:10.3390/ijms241512059. https://pubmed.ncbi.nlm.nih.gov/37569434/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen