C57BL/6JCya-Snx9em1flox/Cya
Common Name:
Snx9-flox
Product ID:
S-CKO-13197
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Snx9-flox
Strain ID
CKOCMP-66616-Snx9-B6J-VA
Gene Name
Product ID
S-CKO-13197
Gene Alias
2700073N08Rik; SDP1; SH3PX1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Snx9em1flox/Cya mice (Catalog S-CKO-13197) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000002436
NCBI RefSeq
NM_025664
Target Region
Exon 5
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Snx9, or sorting nexin-9, is a multifunctional protein involved in fundamental cellular activities. It participates in endocytosis, coordinating membrane trafficking and remodeling with actin dynamics [2,5]. Snx9 binds strongly to dynamin, recruits it to endocytosis sites, modulates the membrane for endocytic vesicle formation, stimulates dynamin's GTPase activity for scission, and activates N-WASP to coordinate actin polymerization with vesicle release [2]. It is also involved in pathways like T-cell activation and GPCR endocytosis [3,4].
In CD8 T cells, gene knockout experiments revealed that deletion of Snx9 alleviates T-cell exhaustion. It decreases PLCγ1, Ca2+, and NFATc2-mediated T-cell signaling, reduces expression of NR4A1/3 and TOX, enhances memory differentiation and IFNγ secretion of adoptively transferred T cells, and improves the anti-tumor efficacy of human chimeric antigen receptor T cells in vivo [1]. In Autosomal Dominant Polycystic Kidney Disease (ADPKD), Snx9 expression is downregulated. Ectopic expression of Snx9 inhibits ADPKD cell proliferation and cyst formation by interacting with YAP, increasing its phosphorylation, and inhibiting Hippo target gene expression [6].
In conclusion, Snx9 is crucial for multiple cellular processes such as endocytosis, T-cell function, and cell proliferation regulation. Gene knockout models have been instrumental in uncovering its role in diseases like T-cell exhaustion-related cancers and ADPKD, providing potential therapeutic targets for these conditions.
References:
1. Trefny, Marcel P, Kirchhammer, Nicole, Auf der Maur, Priska, Hess, Christoph, Zippelius, Alfred. 2023. Deletion of SNX9 alleviates CD8 T cell exhaustion for effective cellular cancer immunotherapy. In Nature communications, 14, 86. doi:10.1038/s41467-022-35583-w. https://pubmed.ncbi.nlm.nih.gov/36732507/
2. Lundmark, Richard, Carlsson, Sven R. . SNX9 - a prelude to vesicle release. In Journal of cell science, 122, 5-11. doi:10.1242/jcs.037135. https://pubmed.ncbi.nlm.nih.gov/19092055/
3. Ecker, Manuela, Schregle, Richard, Kapoor-Kaushik, Natasha, Redpath, Gregory Mi, Rossy, Jeremie. 2022. SNX9-induced membrane tubulation regulates CD28 cluster stability and signalling. In eLife, 11, . doi:10.7554/eLife.67550. https://pubmed.ncbi.nlm.nih.gov/35050850/
4. Robleto, Valeria L, Zhuo, Ya, Crecelius, Joseph M, Benzow, Sara, Marchese, Adriano. 2024. SNX9 family mediates βarrestin-independent GPCR endocytosis. In Communications biology, 7, 1455. doi:10.1038/s42003-024-07157-7. https://pubmed.ncbi.nlm.nih.gov/39511325/
5. Bendris, Nawal, Schmid, Sandra L. 2016. Endocytosis, Metastasis and Beyond: Multiple Facets of SNX9. In Trends in cell biology, 27, 189-200. doi:10.1016/j.tcb.2016.11.001. https://pubmed.ncbi.nlm.nih.gov/27989654/
6. Shen, Ai-Wen, Fu, Li-Li, Lin, Lu, Yin, Pei-Ran, Yu, Sheng-Qiang. 2020. SNX9 Inhibits Cell Proliferation and Cyst Development in Autosomal Dominant Polycystic Kidney Disease via Activation of the Hippo-YAP Signaling Pathway. In Frontiers in cell and developmental biology, 8, 811. doi:10.3389/fcell.2020.00811. https://pubmed.ncbi.nlm.nih.gov/32974348/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen