C57BL/6JCya-Srp72em1flox/Cya
Common Name:
Srp72-flox
Product ID:
S-CKO-13228
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Srp72-flox
Strain ID
CKOCMP-66661-Srp72-B6J-VA
Gene Name
Product ID
S-CKO-13228
Gene Alias
5730576P14Rik; 72kDa
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Srp72em1flox/Cya mice (Catalog S-CKO-13228) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000101087
NCBI RefSeq
NM_025691
Target Region
Exon 3~6
Size of Effective Region
~4.7 kb
Detailed Document
Overview of Gene Research
Srp72, a component of the signal recognition particle (SRP) complex, is crucial for targeting proteins to the endoplasmic reticulum, a key process in co-translational protein targeting and membrane protein insertion [2,3]. SRP72 forms a heterodimer with SRP68, and together they bind to a regulatory site of the SRP RNA [3]. Understanding its function is essential for uncovering the molecular mechanisms of protein translocation and related cellular processes. Mouse models can provide valuable insights into its in-vivo functions.
A study developed an Srp72 null mouse model to investigate its role in the hematopoietic system. Heterozygous loss of Srp72 in mice led to mild reductions in blood and bone marrow cellularity and minor changes within the stem/progenitor compartment, with no observed hematological disorder. Gene expression analysis showed that genes encoding secreted factors were transcriptionally down-regulated in Srp72+/- animals. However, this mouse model only partially recapitulated the phenotype seen in families with inherited SRP72 lesions [1].
In conclusion, Srp72 is essential for protein targeting to the endoplasmic reticulum. The Srp72 null mouse model has provided some understanding of its role in the hematopoietic system. In humans, germline mutations in SRP72 are associated with myelodysplasia and bone marrow aplasia, highlighting its significance in certain hematological diseases [1,4].
References:
1. D'Altri, Teresa, Schuster, Mikkel B, Wenzel, Anne, Porse, Bo T. 2019. Heterozygous loss of Srp72 in mice is not associated with major hematological phenotypes. In European journal of haematology, 103, 319-328. doi:10.1111/ejh.13286. https://pubmed.ncbi.nlm.nih.gov/31254415/
2. Gao, Yina, Zhang, Qi, Lang, Yue, Tong, Yufeng, Chen, Zhongzhou. . Human apo-SRP72 and SRP68/72 complex structures reveal the molecular basis of protein translocation. In Journal of molecular cell biology, 9, 220-230. doi:10.1093/jmcb/mjx010. https://pubmed.ncbi.nlm.nih.gov/28369529/
3. Becker, Matthias M M, Lapouge, Karine, Segnitz, Bernd, Wild, Klemens, Sinning, Irmgard. 2016. Structures of human SRP72 complexes provide insights into SRP RNA remodeling and ribosome interaction. In Nucleic acids research, 45, 470-481. doi:10.1093/nar/gkw1124. https://pubmed.ncbi.nlm.nih.gov/27899666/
4. Kirwan, Michael, Walne, Amanda J, Plagnol, Vincent, Vulliamy, Tom, Dokal, Inderjeet. 2012. Exome sequencing identifies autosomal-dominant SRP72 mutations associated with familial aplasia and myelodysplasia. In American journal of human genetics, 90, 888-92. doi:10.1016/j.ajhg.2012.03.020. https://pubmed.ncbi.nlm.nih.gov/22541560/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen