C57BL/6JCya-Rbm22em1flox/Cya
Common Name:
Rbm22-flox
Product ID:
S-CKO-13307
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rbm22-flox
Strain ID
CKOCMP-66810-Rbm22-B6J-VA
Gene Name
Product ID
S-CKO-13307
Gene Alias
8430430L24Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rbm22em1flox/Cya mice (Catalog S-CKO-13307) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025506
NCBI RefSeq
NM_025776
Target Region
Exon 3~4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Rbm22, a gene encoding an RNA-Binding Protein (RBP) and belonging to the RNA-Binding Motif (RBM) family, plays crucial roles in pre-mRNA splicing and gene expression regulation [1]. Structurally, it has a Zinc Finger like and a Zinc Finger domain, an RNA-Recognition Motif (RRM), and a Proline-Rich domain, suggesting an evolutionary fusion of two yeast genes. It is essential for maintaining the conformation of the spliceosome's catalytic core and acts as a bridge within the spliceosome. Rbm22 is also involved in gene regulation, being able to bind DNA and act as a transcription factor [1].
In zebrafish, knockdown of Rbm22 leads to a truncated axis and developmental arrest of the head and tail, indicating its importance in early embryogenesis [4]. In myeloid cells, Rbm22-depletion delays progression through all steps of the cell cycle and increases ploidy, and is partially responsible for the cytopenia phenotype in myelodysplastic syndromes with a partial deletion of chromosome 5 [5]. In prostate cancer, Rbm22 levels are down-regulated, and its overexpression decreases cancer aggressiveness by dysregulating the splicing of numerous genes and down-regulating critical upstream regulators of the cell-cycle [2]. In non-small cell lung cancer, Rbm22 suppresses tumorigenesis by stabilizing LATS1 mRNA [3].
In conclusion, Rbm22 is essential for pre-mRNA splicing, gene expression regulation, and normal development. Its dysregulation is associated with various diseases, especially cancers. Studies using model organisms like zebrafish and in cell-based functional studies in cancer cell lines have provided insights into its role in biological processes and disease conditions, highlighting its potential as a therapeutic target in cancer [1,2,3,4,5].
References:
1. Soubise, Benoît, Jiang, Yan, Douet-Guilbert, Nathalie, Troadec, Marie-Bérengère. 2022. RBM22, a Key Player of Pre-mRNA Splicing and Gene Expression Regulation, Is Altered in Cancer. In Cancers, 14, . doi:10.3390/cancers14030643. https://pubmed.ncbi.nlm.nih.gov/35158909/
2. Jiménez-Vacas, Juan M, Montero-Hidalgo, Antonio J, Gómez-Gómez, Enrique, Gahete, Manuel D, Luque, Raul M. 2022. Tumor suppressor role of RBM22 in prostate cancer acting as a dual-factor regulating alternative splicing and transcription of key oncogenic genes. In Translational research : the journal of laboratory and clinical medicine, 253, 68-79. doi:10.1016/j.trsl.2022.08.016. https://pubmed.ncbi.nlm.nih.gov/36089245/
3. Hou, Min, Huang, Qingmei, Chen, Shan, Lei, Jing, Zhang, Yakun. 2024. RNA binding protein RBM22 suppresses non-small cell lung cancer tumorigenesis by stabilizing LATS1 mRNA. In Journal of molecular histology, 56, 15. doi:10.1007/s10735-024-10285-3. https://pubmed.ncbi.nlm.nih.gov/39612045/
4. He, F, Wang, C-T, Gou, L-T. 2009. RNA-binding motif protein RBM22 is required for normal development of zebrafish embryos. In Genetics and molecular research : GMR, 8, 1466-73. doi:10.4238/vol8-4gmr683. https://pubmed.ncbi.nlm.nih.gov/20013661/
5. Le Hir-Reynaud, Eloïse, Soubise, Benoît, Mendoza, Abrahan Molina, Douet-Guilbert, Nathalie, Troadec, Marie-Bérengère. 2025. RBM22-depletion delays progression through all steps of cell cycle and increases ploidy in myeloid cells. In Biochimica et biophysica acta. Molecular cell research, 1872, 119965. doi:10.1016/j.bbamcr.2025.119965. https://pubmed.ncbi.nlm.nih.gov/40268057/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen