C57BL/6JCya-Pycardem1flox/Cya
Common Name:
Pycard-flox
Product ID:
S-CKO-13320
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Pycard-flox
Strain ID
CKOCMP-66824-Pycard-B6J-VA
Gene Name
Product ID
S-CKO-13320
Gene Alias
9130417A21Rik; Asc; CARD5; TMS-1; TNS1; masc
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pycardem1flox/Cya mice (Catalog S-CKO-13320) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033056
NCBI RefSeq
NM_023258
Target Region
Exon 1~3
Size of Effective Region
~4.2 kb
Detailed Document
Overview of Gene Research
Pycard, also known as PYD and CARD domain containing or ASC (apoptosis-associated speck-like protein containing a caspase activation and recruitment domain), is a pivotal adaptor protein in inflammasome assembly and activation. It contributes to innate immunity and is involved in multiple pathways such as NOD-like receptor signaling pathway. It plays an essential role in the pathogenesis of various diseases including atherosclerosis, restenosis, tendinopathy, COPD, rheumatoid arthritis, and psoriasis [1-3, 6, 10].
In tendinopathy, pristimerin alleviates the disease by promoting the autophagic degradation of AIM2 through a PYCARD/ASC-dependent manner, enhancing the K63-linked ubiquitin chains of PYCARD/ASC at K158/161 [1]. In COPD, PYCARD is a crucial PANoptosis-related gene, positively related to NOD-like receptor signaling pathway and promoting immune cell infiltration [2]. In the context of neointima formation, Pycard deficiency in knockout mice reduces circulating miRNA profile, inhibits Mir17 seed family maturation, and prevents vascular injury-induced neointima formation by promoting chaperone-mediated autophagic degradation of AGO2 in adipose tissue [3].
In conclusion, Pycard is a key gene in inflammasome-related processes and innate immunity. Studies using gene knockout mouse models have revealed its roles in multiple disease areas, including tendinopathy, COPD, and neointima formation. Understanding Pycard's functions provides potential therapeutic targets for these diseases.
References:
1. Jiang, Huaji, Xie, Yingchao, Lu, Jiansen, Gong, Xiaoqian, Yu, Xiao. 2023. Pristimerin suppresses AIM2 inflammasome by modulating AIM2-PYCARD/ASC stability via selective autophagy to alleviate tendinopathy. In Autophagy, 20, 76-93. doi:10.1080/15548627.2023.2249392. https://pubmed.ncbi.nlm.nih.gov/37647255/
2. Shi, Rui, Liang, Renwen, Wang, Fang, Chen, Alex F, Yang, Weimin. 2024. Identification and experimental validation of PYCARD as a crucial PANoptosis-related gene for immune response and inflammation in COPD. In Apoptosis : an international journal on programmed cell death, 29, 2091-2107. doi:10.1007/s10495-024-01961-6. https://pubmed.ncbi.nlm.nih.gov/38652339/
3. Li, Jian, Yao, Hongmin, Zhao, Fujie, Zou, Ming-Hui, Xie, Zhonglin. 2023. Pycard deficiency inhibits microRNA maturation and prevents neointima formation by promoting chaperone-mediated autophagic degradation of AGO2/argonaute 2 in adipose tissue. In Autophagy, 20, 629-644. doi:10.1080/15548627.2023.2277610. https://pubmed.ncbi.nlm.nih.gov/37963060/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen