C57BL/6JCya-Fuca2em1flox/Cya
Common Name:
Fuca2-flox
Product ID:
S-CKO-13335
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Fuca2-flox
Strain ID
CKOCMP-66848-Fuca2-B6J-VA
Gene Name
Product ID
S-CKO-13335
Gene Alias
0610025O11Rik; 5530401P20Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fuca2em1flox/Cya mice (Catalog S-CKO-13335) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000121465
NCBI RefSeq
NM_025799
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
FUCA2, or alpha-L-fucosidase 2, is a member of the glycoside hydrolase (GH) families 29A. It is involved in the hydrolysis of terminal L-fucose residues in oligosaccharide chains, which contributes to various biological processes. Aberrant fucosylation related to FUCA2 has implications in disease development, including its role in cell-extracellular matrix interactions and cell signaling pathways [5].
In cancer research, FUCA2 shows upregulation in most tumors and is associated with poor survival rates. Knockdown of FUCA2 in lung cancer cells inhibited cell viability, indicating its potential oncogene role [1]. In triple-negative breast cancer (TNBC), cancer-associated adipocytes secrete FUCA2, which promotes TNBC growth and metastasis through interaction with TM9SF3 [2]. In gastric cancer, FUCA2 expression was markedly increased in tumor tissues compared with non-tumoral tissues and was associated with advanced surgical staging and histological grade [3]. In hepatocellular carcinoma, increased FUCA2 expression is related to age, overall survival, and immune infiltration [4]. In lung adenocarcinoma, FUCA2 upregulates GGH to modulate cell cycle and epithelial-mesenchymal transition, promoting tumor progression [6].
In conclusion, FUCA2 is closely associated with cancer progression, with its upregulation often linked to poor prognosis in multiple cancer types. The findings from in vitro cell-based knockdown experiments and in vivo studies in cancer models have revealed its oncogenic role, highlighting its potential as a therapeutic target in cancer treatment.
References:
1. Zhong, Anyuan, Chen, Ting, Xing, Yufei, Pan, Xue, Shi, Minhua. 2021. FUCA2 Is a Prognostic Biomarker and Correlated With an Immunosuppressive Microenvironment in Pan-Cancer. In Frontiers in immunology, 12, 758648. doi:10.3389/fimmu.2021.758648. https://pubmed.ncbi.nlm.nih.gov/34745134/
2. Liu, Qun, Dong, Hui-Ting, Zhao, Tingting, Jin, Feng, Xing, Peng. 2022. Cancer-associated adipocytes release FUCA2 to promote aggressiveness in TNBC. In Endocrine-related cancer, 29, 139-149. doi:10.1530/ERC-21-0243. https://pubmed.ncbi.nlm.nih.gov/34935631/
3. Leal Quirino, Michael Williames, Albuquerque, Amanda Pinheiro de Barros, de Souza, Maria de Fátima Deodato, Pereira, Michelly Cristiny, de Melo, Moacyr Jesus Barreto. . FUCA2 and TSTA3 expression in gastric cancer: candidate biomarkers of malignant transformation. In Folia histochemica et cytobiologica, 60, 335-343. doi:10.5603/FHC.a2022.0031. https://pubmed.ncbi.nlm.nih.gov/36583336/
4. Hu, Yuanhui, Tang, Dongling, Zhang, Pingan. 2023. Prognostic and immunological role of alpha-L-fucosidase 2 (FUCA2) in hepatocellular carcinoma. In Translational cancer research, 12, 257-272. doi:10.21037/tcr-22-1850. https://pubmed.ncbi.nlm.nih.gov/36915579/
5. Fu, Jinxing, Guo, Qing, Feng, Yuan, Cheng, Peng, Wu, Anhua. 2022. Dual role of fucosidase in cancers and its clinical potential. In Journal of Cancer, 13, 3121-3132. doi:10.7150/jca.75840. https://pubmed.ncbi.nlm.nih.gov/36046653/
6. Peng, Yuanyuan, Yang, Xingyu, Liu, Yafeng, Wu, Jing, Hu, Dong. 2024. The regulation of the cell cycle and epithelial-mesenchymal transition through FUCA2/GGH signaling promotes the progression of lung adenocarcinoma. In Gene, 939, 149183. doi:10.1016/j.gene.2024.149183. https://pubmed.ncbi.nlm.nih.gov/39710011/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen