C57BL/6NCya-Lztr1em1flox/Cya
Common Name:
Lztr1-flox
Product ID:
S-CKO-13344
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Lztr1-flox
Strain ID
CKOCMP-66863-Lztr1-B6N-VA
Gene Name
Product ID
S-CKO-13344
Gene Alias
1200003E21Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Lztr1em1flox/Cya mice (Catalog S-CKO-13344) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023444
NCBI RefSeq
NM_025808
Target Region
Exon 9~11
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
LZTR1, or leucine zipper-like transcriptional regulator 1, is an adaptor for the cullin 3 (CUL3) ubiquitin ligase complex. It plays a crucial role in regulating RAS ubiquitination, which in turn impacts the mitogen-activated protein kinase (MAPK) pathway. By modulating RAS signaling, LZTR1 is involved in various biological processes, and its dysregulation is associated with human diseases [1,2].
LZTR1 haploinsufficiency in mice recapitulates Noonan syndrome phenotypes, while LZTR1 loss in Schwann cells drives dedifferentiation and proliferation. Loss of Lztr1 in mice abrogates Ras ubiquitination at lysine-170, and LZTR1-mediated ubiquitination inhibits RAS signaling by attenuating its association with the membrane. Disease-associated LZTR1 mutations disrupt either LZTR1-CUL3 complex formation or its interaction with RAS proteins [1]. Conditional inactivation of Lztr1 and Cdkn2a in the mouse nervous system causes tumors in the peripheral nervous system, recapitulating aspects of schwannomatosis [3].
In conclusion, LZTR1 is essential for regulating RAS ubiquitination and MAPK pathway activation. Studies using mouse models, such as gene knockout (KO) and conditional knockout (CKO) models, have revealed its role in Noonan syndrome and schwannomatosis. These models provide valuable insights into the mechanisms of LZTR1-related diseases, facilitating a better understanding of the underlying biological processes and potentially guiding the development of therapeutic strategies for these conditions.
References:
1. Steklov, M, Pandolfi, S, Baietti, M F, Eyckerman, S, Sablina, A A. 2018. Mutations in LZTR1 drive human disease by dysregulating RAS ubiquitination. In Science (New York, N.Y.), 362, 1177-1182. doi:10.1126/science.aap7607. https://pubmed.ncbi.nlm.nih.gov/30442762/
2. Bigenzahn, Johannes W, Collu, Giovanna M, Kartnig, Felix, Mlodzik, Marek, Superti-Furga, Giulio. 2018. LZTR1 is a regulator of RAS ubiquitination and signaling. In Science (New York, N.Y.), 362, 1171-1177. doi:10.1126/science.aap8210. https://pubmed.ncbi.nlm.nih.gov/30442766/
3. Ko, Aram, Hasanain, Mohammad, Oh, Young Taek, Iavarone, Antonio, Lasorella, Anna. . LZTR1 Mutation Mediates Oncogenesis through Stabilization of EGFR and AXL. In Cancer discovery, 13, 702-723. doi:10.1158/2159-8290.CD-22-0376. https://pubmed.ncbi.nlm.nih.gov/36445254/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen