C57BL/6JCya-Pcyox1em1flox/Cya
Common Name:
Pcyox1-flox
Product ID:
S-CKO-13359
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Pcyox1-flox
Strain ID
CKOCMP-66881-Pcyox1-B6J-VA
Gene Name
Product ID
S-CKO-13359
Gene Alias
1200015P13Rik; PCL1; Pcly
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pcyox1em1flox/Cya mice (Catalog S-CKO-13359) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032065
NCBI RefSeq
NM_025823
Target Region
Exon 3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Pcyox1, known as prenylcysteine oxidase 1, is an enzyme involved in the degradation of prenylated proteins. It hydrolyzes the thioether bond of prenylcysteines in the final step of prenylated protein degradation, releasing hydrogen peroxide, cysteine, and the isoprenoid aldehyde [4]. It is expressed in different tissues such as vascular and blood cells, adipose tissue, liver, gastrointestinal tract, kidney, male reproductive tissue, and muscle [4].
Pcyox1 -/- mice have shown significant phenotypes. In thrombosis studies, FeCl3-induced carotid artery thrombus formation was delayed, and these mice were protected from collagen/epinephrine-induced thromboembolism. Pcyox1 deletion reduced platelet/leukocyte aggregates, platelet aggregation, alpha-granules release, and αIIbβ3 integrin activation in response to agonists [1]. In adipogenesis, Pcyox1 -/-/Apoe -/- mice on a high-fat diet had lower body weight due to reduced visceral adipose content. Pcyox1 gene silencing in 3T3-L1 preadipocytes prevented their differentiation, acting as an upstream regulator of key adipogenic players like FABP4, PPARγ, and C/EBPα [2]. In atherosclerosis, Pcyox1 deficiency in Apoe -/- mice retarded atheroprogression, decreased lesion vulnerability, reduced plasma lipid levels and inflammation [3].
In conclusion, Pcyox1 plays crucial roles in thrombosis, adipogenesis, and atherosclerosis. The use of Pcyox1 gene-knockout mouse models has revealed its functions in these biological processes and disease conditions, suggesting it could be a potential target for antithrombotic drugs and a key factor in understanding adipogenesis and atherosclerosis [1,2,3].
References:
1. Banfi, Cristina, Amadio, Patrizia, Zarà, Marta, Sandrini, Leonardo, Barbieri, Silvia S. 2022. Prenylcysteine Oxidase 1 (PCYOX1), a New Player in Thrombosis. In International journal of molecular sciences, 23, . doi:10.3390/ijms23052831. https://pubmed.ncbi.nlm.nih.gov/35269975/
2. Banfi, Cristina, Mallia, Alice, Ghilardi, Stefania, Sahlén, Pelin, Baetta, Roberta. 2023. Prenylcysteine Oxidase 1 Is a Key Regulator of Adipogenesis. In Antioxidants (Basel, Switzerland), 12, . doi:10.3390/antiox12030542. https://pubmed.ncbi.nlm.nih.gov/36978789/
3. Banfi, C, Baetta, R, Barbieri, S S, Eriksson, P, Tremoli, E. 2021. Prenylcysteine oxidase 1, an emerging player in atherosclerosis. In Communications biology, 4, 1109. doi:10.1038/s42003-021-02630-z. https://pubmed.ncbi.nlm.nih.gov/34548610/
4. Herrera-Marcos, Luis V, Lou-Bonafonte, Jose M, Martinez-Gracia, Maria V, Navarro, María A, Osada, Jesus. 2018. Prenylcysteine oxidase 1, a pro-oxidant enzyme of low density lipoproteins. In Frontiers in bioscience (Landmark edition), 23, 1020-1037. doi:. https://pubmed.ncbi.nlm.nih.gov/28930587/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen