C57BL/6NCya-Trmt6em1flox/Cya
Common Name:
Trmt6-flox
Product ID:
S-CKO-13391
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Trmt6-flox
Strain ID
CKOCMP-66926-Trmt6-B6N-VA
Gene Name
Product ID
S-CKO-13391
Gene Alias
3300001M20Rik; CGI-09; mKIAA1153
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Trmt6em1flox/Cya mice (Catalog S-CKO-13391) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000039554
NCBI RefSeq
NM_175113
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
TRMT6, short for tRNA methyltransferase 6, is an enzyme that catalyzes N1-methyladenosine (m1A), a reversible modification in various RNAs such as tRNA, mRNA, rRNA, and lncRNA. This modification is crucial as it participates in regulating RNA processing, structure, and functions. The TRMT6-TRMT61A complex, in particular, is involved in the m1A methylation of tRNA, which impacts diverse biological processes and is associated with multiple disease-related pathways [1,2,4,5,6,7,9].
In aged murine hematopoietic stem cells (HSCs), an increase in the TRMT6-TRMT61A complex due to declined CRL4DCAF1-mediated ubiquitination degradation signaling impairs HSCs through 3'-tiRNA-Leu-CAG and the RIPK1-RIPK3-MLKL-mediated necroptosis cascade [2]. In hepatocellular carcinoma (HCC), TRMT6 is overexpressed, promoting cell proliferation both in vivo and in vitro through the PI3K/AKT/mTOR axis [3]. Also, in HCC, TRMT6/TRMT61A-mediated m1A methylation in tRNA drives tumourigenesis by regulating cholesterol metabolism [4]. In bladder cancer, depletion of TRMT6/TRMT61A reduces proliferation and resistance against cellular stress [7]. In addition, the rs236110 C > A polymorphism in the TRMT6 gene increases the risk of Wilms tumor [8].
In conclusion, TRMT6, mainly as part of the TRMT6-TRMT61A complex, plays a significant role in m1A methylation of tRNA, influencing diverse biological processes. Model-based research, especially studies using KO/CKO mouse models, has revealed its importance in diseases like HSC aging-related impairments, hepatocellular carcinoma, bladder cancer, and Wilms tumor, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Su, Zhangli, Monshaugen, Ida, Wilson, Briana, Ougland, Rune, Dutta, Anindya. 2022. TRMT6/61A-dependent base methylation of tRNA-derived fragments regulates gene-silencing activity and the unfolded protein response in bladder cancer. In Nature communications, 13, 2165. doi:10.1038/s41467-022-29790-8. https://pubmed.ncbi.nlm.nih.gov/35444240/
2. He, Hanqing, Wang, Yuqian, Zhang, Xiaoting, Yi, Chengqi, Wang, Jianwei. 2024. Age-related noncanonical TRMT6-TRMT61A signaling impairs hematopoietic stem cells. In Nature aging, 4, 213-230. doi:10.1038/s43587-023-00556-1. https://pubmed.ncbi.nlm.nih.gov/38233630/
3. Ye, Yanqing, Liu, Maosheng, Wu, Fengfei, Xie, Fang, Bai, Lan. 2023. TRMT6 promotes hepatocellular carcinoma progression through the PI3K/AKT signaling pathway. In European journal of medical research, 28, 48. doi:10.1186/s40001-022-00951-1. https://pubmed.ncbi.nlm.nih.gov/36707905/
4. Wang, Yanying, Wang, Jing, Li, Xiaoyu, Yi, Chengqi, Fan, Zusen. 2021. N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism. In Nature communications, 12, 6314. doi:10.1038/s41467-021-26718-6. https://pubmed.ncbi.nlm.nih.gov/34728628/
5. Zuo, Hongna, Wu, Aiwei, Wang, Mingwei, Hong, Liquan, Wang, Hu. 2024. tRNA m1A modification regulate HSC maintenance and self-renewal via mTORC1 signaling. In Nature communications, 15, 5706. doi:10.1038/s41467-024-50110-9. https://pubmed.ncbi.nlm.nih.gov/38977676/
6. Li, Jiexin, Zhang, Haisheng, Wang, Hongsheng. 2022. N1-methyladenosine modification in cancer biology: Current status and future perspectives. In Computational and structural biotechnology journal, 20, 6578-6585. doi:10.1016/j.csbj.2022.11.045. https://pubmed.ncbi.nlm.nih.gov/36467585/
7. Monshaugen, Ida, Luna, Luisa, Rhodes, Jayden, Klungland, Arne, Ougland, Rune. 2024. Depletion of the m1A writer TRMT6/TRMT61A reduces proliferation and resistance against cellular stress in bladder cancer. In Frontiers in oncology, 13, 1334112. doi:10.3389/fonc.2023.1334112. https://pubmed.ncbi.nlm.nih.gov/38304034/
8. Chang, Xiaofeng, Zhu, Jinhong, Hua, Rui-Xi, He, Jing, Wang, Huanmin. 2023. TRMT6 gene rs236110 C > A polymorphism increases the risk of Wilms tumor. In Gene, 882, 147646. doi:10.1016/j.gene.2023.147646. https://pubmed.ncbi.nlm.nih.gov/37473973/
9. Liu, Yongbo, Zhou, Jing, Li, Xiaoyu, Wu, Yuzhang, Li, Hua-Bing. 2022. tRNA-m1A modification promotes T cell expansion via efficient MYC protein synthesis. In Nature immunology, 23, 1433-1444. doi:10.1038/s41590-022-01301-3. https://pubmed.ncbi.nlm.nih.gov/36138184/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen