C57BL/6NCya-Trmt6em1flox/Cya
Common Name
Trmt6-flox
Product ID
S-CKO-13391
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-66926-Trmt6-B6N-VA
When using this mouse strain in a publication, please cite “Trmt6-flox Mouse (Catalog S-CKO-13391) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Trmt6-flox
Strain ID
CKOCMP-66926-Trmt6-B6N-VA
Gene Name
Product ID
S-CKO-13391
Gene Alias
3300001M20Rik, CGI-09, mKIAA1153
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000039554
NCBI RefSeq
NM_175113
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Overview of Gene Research
TRMT6, short for tRNA methyltransferase 6, is an enzyme that catalyzes N1-methyladenosine (m1A), a reversible modification in various RNAs such as tRNA, mRNA, rRNA, and lncRNA. This modification is crucial as it participates in regulating RNA processing, structure, and functions. The TRMT6-TRMT61A complex, in particular, is involved in the m1A methylation of tRNA, which impacts diverse biological processes and is associated with multiple disease-related pathways [1,2,4,5,6,7,9].
In aged murine hematopoietic stem cells (HSCs), an increase in the TRMT6-TRMT61A complex due to declined CRL4DCAF1-mediated ubiquitination degradation signaling impairs HSCs through 3'-tiRNA-Leu-CAG and the RIPK1-RIPK3-MLKL-mediated necroptosis cascade [2]. In hepatocellular carcinoma (HCC), TRMT6 is overexpressed, promoting cell proliferation both in vivo and in vitro through the PI3K/AKT/mTOR axis [3]. Also, in HCC, TRMT6/TRMT61A-mediated m1A methylation in tRNA drives tumourigenesis by regulating cholesterol metabolism [4]. In bladder cancer, depletion of TRMT6/TRMT61A reduces proliferation and resistance against cellular stress [7]. In addition, the rs236110 C > A polymorphism in the TRMT6 gene increases the risk of Wilms tumor [8].
In conclusion, TRMT6, mainly as part of the TRMT6-TRMT61A complex, plays a significant role in m1A methylation of tRNA, influencing diverse biological processes. Model-based research, especially studies using KO/CKO mouse models, has revealed its importance in diseases like HSC aging-related impairments, hepatocellular carcinoma, bladder cancer, and Wilms tumor, providing insights into disease mechanisms and potential therapeutic targets.
References：
1. Su, Zhangli, Monshaugen, Ida, Wilson, Briana, Ougland, Rune, Dutta, Anindya. 2022. TRMT6/61A-dependent base methylation of tRNA-derived fragments regulates gene-silencing activity and the unfolded protein response in bladder cancer. In Nature communications, 13, 2165. doi:10.1038/s41467-022-29790-8. https://pubmed.ncbi.nlm.nih.gov/35444240/
2. He, Hanqing, Wang, Yuqian, Zhang, Xiaoting, Yi, Chengqi, Wang, Jianwei. 2024. Age-related noncanonical TRMT6-TRMT61A signaling impairs hematopoietic stem cells. In Nature aging, 4, 213-230. doi:10.1038/s43587-023-00556-1. https://pubmed.ncbi.nlm.nih.gov/38233630/
3. Ye, Yanqing, Liu, Maosheng, Wu, Fengfei, Xie, Fang, Bai, Lan. 2023. TRMT6 promotes hepatocellular carcinoma progression through the PI3K/AKT signaling pathway. In European journal of medical research, 28, 48. doi:10.1186/s40001-022-00951-1. https://pubmed.ncbi.nlm.nih.gov/36707905/
4. Wang, Yanying, Wang, Jing, Li, Xiaoyu, Yi, Chengqi, Fan, Zusen. 2021. N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism. In Nature communications, 12, 6314. doi:10.1038/s41467-021-26718-6. https://pubmed.ncbi.nlm.nih.gov/34728628/
5. Zuo, Hongna, Wu, Aiwei, Wang, Mingwei, Hong, Liquan, Wang, Hu. 2024. tRNA m1A modification regulate HSC maintenance and self-renewal via mTORC1 signaling. In Nature communications, 15, 5706. doi:10.1038/s41467-024-50110-9. https://pubmed.ncbi.nlm.nih.gov/38977676/
6. Li, Jiexin, Zhang, Haisheng, Wang, Hongsheng. 2022. N1-methyladenosine modification in cancer biology: Current status and future perspectives. In Computational and structural biotechnology journal, 20, 6578-6585. doi:10.1016/j.csbj.2022.11.045. https://pubmed.ncbi.nlm.nih.gov/36467585/
7. Monshaugen, Ida, Luna, Luisa, Rhodes, Jayden, Klungland, Arne, Ougland, Rune. 2024. Depletion of the m1A writer TRMT6/TRMT61A reduces proliferation and resistance against cellular stress in bladder cancer. In Frontiers in oncology, 13, 1334112. doi:10.3389/fonc.2023.1334112. https://pubmed.ncbi.nlm.nih.gov/38304034/
8. Chang, Xiaofeng, Zhu, Jinhong, Hua, Rui-Xi, He, Jing, Wang, Huanmin. 2023. TRMT6 gene rs236110 C > A polymorphism increases the risk of Wilms tumor. In Gene, 882, 147646. doi:10.1016/j.gene.2023.147646. https://pubmed.ncbi.nlm.nih.gov/37473973/
9. Liu, Yongbo, Zhou, Jing, Li, Xiaoyu, Wu, Yuzhang, Li, Hua-Bing. 2022. tRNA-m1A modification promotes T cell expansion via efficient MYC protein synthesis. In Nature immunology, 23, 1433-1444. doi:10.1038/s41590-022-01301-3. https://pubmed.ncbi.nlm.nih.gov/36138184/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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