C57BL/6JCya-Aagabem1flox/Cya
Common Name:
Aagab-flox
Product ID:
S-CKO-13400
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Aagab-flox
Strain ID
CKOCMP-66939-Aagab-B6J-VA
Gene Name
Product ID
S-CKO-13400
Gene Alias
2310007F21Rik; p34
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Aagabem1flox/Cya mice (Catalog S-CKO-13400) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041551
NCBI RefSeq
NM_025857
Target Region
Exon 6
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Aagab, also known as alpha-and gamma-adaptin-binding protein (p34), is an assembly chaperone that plays a crucial role in intracellular membrane trafficking. It is involved in regulating the assembly of AP1 and AP2 clathrin adaptors, which are key players in clathrin-mediated transport between the trans-Golgi network and the endosome, as well as clathrin-mediated endocytosis [1,2]. This function is essential for processes like nutrient uptake, signal transduction, and synaptic vesicle recycling [3]. Genetic models such as zebrafish mutants and cell-based models with gene knockdown have been valuable for studying Aagab.
In zebrafish, aagab loss-of-function mutants show impaired swimming and early larval mortality. The mutants display subdued calcium responses and local field potential in optic tectal neurons, along with reduced neurotransmitter release, indicating a role in regulating neural activity [3]. In human cells, AAGAB mutations are associated with punctate palmoplantar keratoderma type 1 (PPKP1), a skin disease. These mutations abolish the function of AAGAB in regulating AP2 adaptor assembly [2,5,6,7]. Additionally, AAGAB promotes AP1 assembly by binding and stabilizing its γ and σ subunits, and its mutation disrupts AP1-mediated cargo trafficking [1]. In AAGAB-knockout cells, there is a reduction in AP-4 subunits and accumulation of ATG9A at the trans-Golgi network, suggesting AAGAB also aids in AP-4 complex assembly [4].
In conclusion, Aagab is a vital assembly chaperone for multiple adaptor complexes, significantly influencing intracellular membrane trafficking. Its function has been illuminated through various model-based research, including zebrafish and cell-based knockout studies. Understanding Aagab's role provides insights into diseases such as PPKP1 and potentially other disorders related to defective membrane trafficking and neural function.
References:
1. Wan, Chun, Crisman, Lauren, Wang, Bing, Yin, Qian, Shen, Jingshi. 2021. AAGAB is an assembly chaperone regulating AP1 and AP2 clathrin adaptors. In Journal of cell science, 134, . doi:10.1242/jcs.258587. https://pubmed.ncbi.nlm.nih.gov/34494650/
2. Gulbranson, Daniel R, Crisman, Lauren, Lee, MyeongSeon, Yu, Haijia, Shen, Jingshi. 2019. AAGAB Controls AP2 Adaptor Assembly in Clathrin-Mediated Endocytosis. In Developmental cell, 50, 436-446.e5. doi:10.1016/j.devcel.2019.06.013. https://pubmed.ncbi.nlm.nih.gov/31353312/
3. Ding, Shihui, Aziz, Tursunjan, Meng, Anming, Jia, Shunji. 2024. Aagab is required for zebrafish larval development by regulating neural activity. In Journal of genetics and genomics = Yi chuan xue bao, 51, 630-641. doi:10.1016/j.jgg.2024.01.003. https://pubmed.ncbi.nlm.nih.gov/38253235/
4. Mattera, Rafael, De Pace, Raffaella, Bonifacino, Juan S. 2022. The adaptor protein chaperone AAGAB stabilizes AP-4 complex subunits. In Molecular biology of the cell, 33, ar109. doi:10.1091/mbc.E22-05-0177. https://pubmed.ncbi.nlm.nih.gov/35976721/
5. Elhaji, Youssef, Hedlin, Cherise, Nath, Anu, Northgrave, Stacey, Hull, Peter R. 2019. AAGAB Mutations in 18 Canadian Families With Punctate Palmoplantar Keratoderma and a Possible Link to Cancer. In Journal of cutaneous medicine and surgery, 24, 28-32. doi:10.1177/1203475419878161. https://pubmed.ncbi.nlm.nih.gov/31526046/
6. Gómez-García, Ana C, Salas-Alanís, Julio C, Bar-Fernandez, Nathaly, Díaz-Montes, Stephane M, Fajardo-Ramírez, Oscar R. . A novel AAGAB mutation in a Peruvian family with punctate palmoplantar keratoderma. In Acta dermatovenerologica Alpina, Pannonica, et Adriatica, 30, 47-48. doi:. https://pubmed.ncbi.nlm.nih.gov/33765759/
7. Zamiri, M, Wilson, N J, Mackenzie, A, Leitch, C, Smith, F J D. 2019. Painful punctate palmoplantar keratoderma due to heterozygous mutations in AAGAB. In The British journal of dermatology, 180, 1250-1251. doi:10.1111/bjd.17442. https://pubmed.ncbi.nlm.nih.gov/30451279/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen