C57BL/6JCya-Shisa5em1flox/Cya
Common Name:
Shisa5-flox
Product ID:
S-CKO-13401
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Shisa5-flox
Strain ID
CKOCMP-66940-Shisa5-B6J-VA
Gene Name
Product ID
S-CKO-13401
Gene Alias
2310008D10Rik; 6430628I05Rik; Scotin; mShisa5
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Shisa5em1flox/Cya mice (Catalog S-CKO-13401) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000112059
NCBI RefSeq
NM_001284332
Target Region
Exon 4~6
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Shisa5, also known as SCOTIN, is involved in restraining spontaneous autophagy induction by blocking the contact between the ER exit site (ERES) and phagophores [1]. It may also be involved in cell signaling and intercellular interactions due to its unique transmembrane domain structure [2]. In addition, it is associated with pathways like endoplasmic reticulum (ER) stress and autophagy [3].
In a study on high-fat stimulation-induced atrial structural remodeling, overexpression of SHISA5 in human cardiac fibroblast cells reduced ER stress, autophagy, and fibrosis. High-fat stimulation increased the expression of tropomyosin 1 (TPM1) in cardiomyocytes, which activated the P53/SHISA5 signaling axis and induced ER stress and autophagy, promoting atrial structural remodeling [3]. In the context of sepsis, SHISA5 was found to be related to significant alternative splicing events and was enriched in the cell apoptosis pathway [4].
In conclusion, Shisa5 plays a role in regulating autophagy, cell signaling, and may be involved in disease-related processes such as atrial structural remodeling and sepsis. Studies on Shisa5 contribute to understanding these biological processes and disease mechanisms, potentially providing new insights for related disease treatments.
References：
1. Lee, Jee-Eun, Kim, Nari, Jung, Minkyo, Mun, Ji-Young, Yoo, Joo-Yeon. 2021. SHISA5/SCOTIN restrains spontaneous autophagy induction by blocking contact between the ERES and phagophores. In Autophagy, 18, 1613-1628. doi:10.1080/15548627.2021.1994297. https://pubmed.ncbi.nlm.nih.gov/34720018/
2. Pan, Tongtong, Wu, Faling, Zhang, Jiarong, Chen, Yongping, Jin, Xiaoya. 2025. The molecular structure of SHISA5 protein and its novel role in primary biliary cholangitis: From single-cell RNA sequencing to biomarkers. In International journal of biological macromolecules, 296, 139775. doi:10.1016/j.ijbiomac.2025.139775. https://pubmed.ncbi.nlm.nih.gov/39800023/
3. Cui, Yansong, Bai, Shuting, Liu, Zhenlin, Li, Zhan, Hou, Yinglong. 2025. High-fat stimulation induces atrial structural remodeling via the TPM1/P53/SHISA5 Axis. In Lipids in health and disease, 24, 138. doi:10.1186/s12944-025-02554-1. https://pubmed.ncbi.nlm.nih.gov/40221727/
4. Tuerdimaimaiti, Dilixiati, Abuduaini, Buzukela, Kang, Shaotao, Tuerhong, Reyila, Kulaxi, Ajiguli. 2023. Genome-wide identification and functional analysis of dysregulated alternative splicing profiles in sepsis. In Journal of inflammation (London, England), 20, 31. doi:10.1186/s12950-023-00355-w. https://pubmed.ncbi.nlm.nih.gov/37749550/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen