C57BL/6JCya-Ddx17em1flox/Cya
Common Name:
Ddx17-flox
Product ID:
S-CKO-13465
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ddx17-flox
Strain ID
CKOCMP-67040-Ddx17-B6J-VA
Gene Name
Product ID
S-CKO-13465
Gene Alias
2610007K22Rik; A430025E01Rik; Gm926; p72
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ddx17em1flox/Cya mice (Catalog S-CKO-13465) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000054014
NCBI RefSeq
NM_199080
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Ddx17, a member of the DEAD-box RNA helicase family, is a nuclear and cytoplasmic shuttle protein with transcriptional cofactor activity [3]. It participates in almost all processes of RNA metabolism, such as mRNA alternative splicing, miRNA and ribosome biogenesis, mRNA degradation, interaction with lncRNAs, and co-regulation of transcriptional activity [3].
In hepatocellular carcinoma (HCC), Ddx17 knockout inhibited extracellular matrix degradation and the invasive ability of HCC cells. A Ddx17-deficient diethylnitrosamine-induced mouse model showed a significant reduction in lung metastasis. High Ddx17 levels induced intron 3 retention of PXN-AS1, producing a metastasis-promoting transcript that activated MYC transcription [1].
In heart failure, cardiomyocyte-specific Ddx17-knockout mice (Ddx17-cKO) showed autophagic flux blockage, cardiomyocyte apoptosis, progressive cardiac dysfunction, and maladaptive remodeling, while restoration of DDX17 expression protected cardiac function. DDX17 binds to BCL6 and inhibits DRP1 expression, maintaining mitochondrial homeostasis [2].
In colorectal cancer, DDX17 promoted cell migration and invasion in vitro and in vivo, and its knockdown had the opposite effect. It regulated the miR-149-3p/CYBRD1 pathway to induce epithelial-mesenchymal transition and metastasis [4].
In conclusion, Ddx17 plays crucial roles in multiple biological processes and diseases. Gene knockout models, such as Ddx17-KO in HCC and Ddx17-cKO in heart failure, have revealed its importance in cancer metastasis and cardiac function maintenance, respectively. These findings suggest that Ddx17 could be a potential target for treating related diseases.
References:
1. Zhou, Hong-Zhong, Li, Fan, Cheng, Sheng-Tao, Huang, Ai-Long, Chen, Juan. 2021. DDX17-regulated alternative splicing that produced an oncogenic isoform of PXN-AS1 to promote HCC metastasis. In Hepatology (Baltimore, Md.), 75, 847-865. doi:10.1002/hep.32195. https://pubmed.ncbi.nlm.nih.gov/34626132/
2. Yan, Mingjing, Gao, Junpeng, Lan, Ming, Li, Jian, Shen, Tao. 2024. DEAD-box helicase 17 (DDX17) protects cardiac function by promoting mitochondrial homeostasis in heart failure. In Signal transduction and targeted therapy, 9, 127. doi:10.1038/s41392-024-01831-2. https://pubmed.ncbi.nlm.nih.gov/38782919/
3. Xu, Kun, Sun, Shenghui, Yan, Mingjing, Li, Jian, Shen, Tao. 2022. DDX5 and DDX17-multifaceted proteins in the regulation of tumorigenesis and tumor progression. In Frontiers in oncology, 12, 943032. doi:10.3389/fonc.2022.943032. https://pubmed.ncbi.nlm.nih.gov/35992805/
4. Zhao, Gang, Wang, Qijing, Zhang, Yue, Mo, Chunfen, Lin, Ping. 2023. DDX17 induces epithelial-mesenchymal transition and metastasis through the miR-149-3p/CYBRD1 pathway in colorectal cancer. In Cell death & disease, 14, 1. doi:10.1038/s41419-022-05508-y. https://pubmed.ncbi.nlm.nih.gov/36593242/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen