C57BL/6JCya-Ola1em1flox/Cya
Common Name:
Ola1-flox
Product ID:
S-CKO-13479
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ola1-flox
Strain ID
CKOCMP-67059-Ola1-B6J-VA
Gene Name
Product ID
S-CKO-13479
Gene Alias
2510025G09Rik; 2810405J23Rik; 2810409H07Rik; Gtpbp9; PTD004
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ola1em1flox/Cya mice (Catalog S-CKO-13479) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028517
NCBI RefSeq
NM_025942
Target Region
Exon 4
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Ola1, or Obg-like ATPase 1, is a highly conserved GTPase that possesses both GTPase and ATPase activities. It is involved in multiple biological processes such as cell proliferation, oxidative response, protein synthesis, and tumorigenesis. Ola1 is associated with pathways like mitochondrial energy metabolism, and its phosphorylation can trigger translocation to the nucleus, affecting gene expression related to mitochondrial bioenergetic function [2]. It also interacts with proteins like BRCA1 and BARD1, participating in centrosome regulation [3].
In colorectal cancer, Ola1 is highly expressed in 84% of cases compared to surrounding tissues, and high expression is an independent factor of poor prognosis. Ola1-KO CRC cell lines show lower growth and tumorigenesis ability, with down-regulation of HIF1α/CA9 axis, suggesting its role in promoting tumorigenesis through this axis [1]. In mouse oocytes, knockdown of Ola1 impairs germinal vesicle breakdown, spindle assembly, and spindle assembly checkpoint (SAC) activation, indicating its importance in oocyte meiosis [4].
In conclusion, Ola1 is crucial for various biological functions, including cell division-related processes and energy metabolism. Its role in promoting tumorigenesis in colorectal cancer and its importance in oocyte meiosis are revealed through gene knockout models. These findings highlight its significance in understanding the mechanisms of cancer development and reproductive biology.
References:
1. Liu, Yue, Kong, Xiang-Xing, He, Jin-Jie, Ding, Ke-Feng, Xu, Dong. 2022. OLA1 promotes colorectal cancer tumorigenesis by activation of HIF1α/CA9 axis. In BMC cancer, 22, 424. doi:10.1186/s12885-022-09508-1. https://pubmed.ncbi.nlm.nih.gov/35440019/
2. Sidlowski, Paul, Czerwinski, Amanda, Liu, Yong, Konduri, Girija G, Afolayan, Adeleye J. . OLA1 Phosphorylation Governs the Mitochondrial Bioenergetic Function of Pulmonary Vascular Cells. In American journal of respiratory cell and molecular biology, 68, 395-405. doi:10.1165/rcmb.2022-0186OC. https://pubmed.ncbi.nlm.nih.gov/36481055/
3. Otsuka, Kei, Yoshino, Yuki, Qi, Huicheng, Chiba, Natsuko. 2020. The Function of BARD1 in Centrosome Regulation in Cooperation with BRCA1/OLA1/RACK1. In Genes, 11, . doi:10.3390/genes11080842. https://pubmed.ncbi.nlm.nih.gov/32722046/
4. Xie, Di, Zhang, Juan, Ding, JinLi, Yang, Jing, Zhang, Yan. 2020. OLA1 is responsible for normal spindle assembly and SAC activation in mouse oocytes. In PeerJ, 8, e8180. doi:10.7717/peerj.8180. https://pubmed.ncbi.nlm.nih.gov/31915569/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen