C57BL/6JCya-Mtdhem1flox/Cya
Common Name:
Mtdh-flox
Product ID:
S-CKO-13538
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mtdh-flox
Strain ID
CKOCMP-67154-Mtdh-B6J-VA
Gene Name
Product ID
S-CKO-13538
Gene Alias
2610103J23Rik; 3D3; 3D3/Lyric; AEG-1; D8Bwg1112e; Lyric
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mtdhem1flox/Cya mice (Catalog S-CKO-13538) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022865
NCBI RefSeq
NM_026002
Target Region
Exon 3~4
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Mtdh, also known as AEG-1 and LYRIC, is a 582-amino-acid transmembrane protein encoded by a gene at chromosome 8q22. It is distributed in the cytoplasm, peri-nuclear region, nucleus, nucleolus, and endoplasmic reticulum. Mtdh is involved in multiple signaling pathways such as PI3K/Akt, NF-κB, Wnt/β-catenin, and MAPK, and is crucial in various biological processes including cell proliferation, invasion, angiogenesis, metastasis, and chemoresistance in cancer [2,3,4].
Genetic ablation of Mtdh in mice has shown that it inhibits breast cancer development by disrupting its interaction with SND1, which is required for breast cancer progression [1]. Mtdh -/- mice are also protected against dextran sodium sulphate-induced colitis symptoms, indicating Mtdh's role in promoting colitis by facilitating TLR-induced pro-inflammatory cytokine production in colonic lamina propria macrophages [5].
In conclusion, Mtdh is a key regulator in cancer progression and intestinal inflammation. Gene knockout mouse models have been instrumental in revealing its role in breast cancer and colitis, providing potential therapeutic targets for these diseases.
References：
1. Shen, Minhong, Wei, Yong, Kim, Hahn, Shao, Zhi-Ming, Kang, Yibin. 2021. Small-molecule inhibitors that disrupt the MTDH-SND1 complex suppress breast cancer progression and metastasis. In Nature cancer, 3, 43-59. doi:10.1038/s43018-021-00279-5. https://pubmed.ncbi.nlm.nih.gov/35121987/
2. Sarkar, Devanand, Fisher, Paul B. . AEG-1/MTDH/LYRIC: clinical significance. In Advances in cancer research, 120, 39-74. doi:10.1016/B978-0-12-401676-7.00002-4. https://pubmed.ncbi.nlm.nih.gov/23889987/
3. Shi, Xue, Wang, Xin. 2015. The role of MTDH/AEG-1 in the progression of cancer. In International journal of clinical and experimental medicine, 8, 4795-807. doi:. https://pubmed.ncbi.nlm.nih.gov/26131054/
4. Abdel Ghafar, Muhammad T, Soliman, Nema A. 2021. Metadherin (AEG-1/MTDH/LYRIC) expression: Significance in malignancy and crucial role in colorectal cancer. In Advances in clinical chemistry, 106, 235-280. doi:10.1016/bs.acc.2021.09.007. https://pubmed.ncbi.nlm.nih.gov/35152973/
5. Wang, Lijuan, Zhang, Ning, Han, Dianwen, Hu, Guohong, Yang, Qifeng. . MTDH Promotes Intestinal Inflammation by Positively Regulating TLR Signalling. In Journal of Crohn's & colitis, 15, 2103-2117. doi:10.1093/ecco-jcc/jjab086. https://pubmed.ncbi.nlm.nih.gov/33987665/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen