C57BL/6JCya-Ndufa10em1flox/Cya
Common Name:
Ndufa10-flox
Product ID:
S-CKO-13603
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ndufa10-flox
Strain ID
CKOCMP-67273-Ndufa10-B6J-VA
Gene Name
Product ID
S-CKO-13603
Gene Alias
2900053E13Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ndufa10em1flox/Cya mice (Catalog S-CKO-13603) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027478
NCBI RefSeq
NM_024197
Target Region
Exon 2~3
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Ndufa10 encodes a protein with NADH dehydrogenase and oxidoreductase activities in the mitochondrial electron transport chain. It is an accessory subunit of mitochondrial complex I, playing a crucial role in maintaining the integrity and function of this complex, thus being essential for mitochondrial oxidative phosphorylation [3].
In a study on diabetic cardiomyopathy, CAV3 overexpression restored mitochondrial function and alleviated the disease partially through NDUFA10. CAV3 interacted with NDUFA10, reduced its lysosomal degradation, restored complex I activity [1].
In NASH research, AGK deficiency-induced liver damage was attributed to hepatic mitochondrial dysfunction as AGK interacted with NDUFA10 to regulate mitochondrial fatty acid metabolism and maintain complex I function [2].
In Alzheimer's disease, lower NDUFA10 expression levels correlated with a higher degree of dementia. The hypothesis was that reduced METTL3 expression decreased the m6A modification level of NDUFA10 mRNA, reducing its protein expression and contributing to complex I assembly disorder [3].
Also, most mitochondrial dGTP binds tightly to NDUFA10 through its deoxyribonucleoside kinase domain, which may regulate mitochondrial dNTP availability [4].
Mutations in Ndufa10 were found to cause complex I deficiency in a patient with Leigh disease, leading to decreased complex I amount, activity, and disturbed assembly [5].
In a Parkinson's cell model, neuroglobin protected cells against apoptosis by interacting with NDUFA10, rescuing complex I activity [6].
Benzo[a]pyrene inhibited testosterone biosynthesis via NDUFA10-mediated mitochondrial impairment in mouse Leydig cells [7].
And knockout of Ndufa10 in CD8+ T cells led to enhanced tumor growth and increased exhaustion of tumor-infiltrating CD8+ T cells [8].
In conclusion, Ndufa10 is essential for mitochondrial complex I function and mitochondrial oxidative phosphorylation. Studies using various models, including gene knockout in CD8+ T cells, have revealed its significance in multiple disease conditions such as diabetic cardiomyopathy, NASH, Alzheimer's disease, Leigh disease, Parkinson's disease, male steroidogenesis disorder, and cancer-related immune dysfunction. These findings provide insights into potential therapeutic targets for these diseases.
References:
1. Guo, Ping, Hu, Shuiqing, Liu, Xiaohui, Fang, Qin, Wang, Yan. 2024. CAV3 alleviates diabetic cardiomyopathy via inhibiting NDUFA10-mediated mitochondrial dysfunction. In Journal of translational medicine, 22, 390. doi:10.1186/s12967-024-05223-6. https://pubmed.ncbi.nlm.nih.gov/38671439/
2. Ding, Nan, Wang, Kang, Jiang, Haojie, Xu, Yanyan, Liu, Junling. 2022. AGK regulates the progression to NASH by affecting mitochondria complex I function. In Theranostics, 12, 3237-3250. doi:10.7150/thno.69826. https://pubmed.ncbi.nlm.nih.gov/35547757/
3. Yang, Lin, Pang, Xinping, Guo, Wenbo, Wang, Kui, Pang, Chaoyang. 2023. An Exploration of the Coherent Effects between METTL3 and NDUFA10 on Alzheimer's Disease. In International journal of molecular sciences, 24, . doi:10.3390/ijms241210111. https://pubmed.ncbi.nlm.nih.gov/37373264/
4. Molina-Granada, David, González-Vioque, Emiliano, Dibley, Marris G, Cámara, Yolanda, Martí, Ramon. 2022. Most mitochondrial dGTP is tightly bound to respiratory complex I through the NDUFA10 subunit. In Communications biology, 5, 620. doi:10.1038/s42003-022-03568-6. https://pubmed.ncbi.nlm.nih.gov/35739187/
5. Hoefs, Saskia J G, van Spronsen, Francjan J, Lenssen, Ellen W H, Smeitink, Jan A M, van den Heuvel, Lambert P. 2010. NDUFA10 mutations cause complex I deficiency in a patient with Leigh disease. In European journal of human genetics : EJHG, 19, 270-4. doi:10.1038/ejhg.2010.204. https://pubmed.ncbi.nlm.nih.gov/21150889/
6. Liang, Xiaomei, Wen, Yutong, Feng, Cuilian, Zhao, Xiaodong, Gao, Xiaoya. 2024. Neuroglobin protects dopaminergic neurons in a Parkinson's cell model by interacting with mitochondrial complex NDUFA10. In Neuroscience, 562, 43-53. doi:10.1016/j.neuroscience.2024.10.033. https://pubmed.ncbi.nlm.nih.gov/39454716/
7. Yang, Wang, Cui, Haonan, Chai, Zili, Ling, Xi, Ao, Lin. 2022. Benzo[a]pyrene inhibits testosterone biosynthesis via NDUFA10-mediated mitochondrial compromise in mouse Leydig cells: Integrating experimental and in silico toxicological approaches. In Ecotoxicology and environmental safety, 244, 114075. doi:10.1016/j.ecoenv.2022.114075. https://pubmed.ncbi.nlm.nih.gov/36108438/
8. Liu, Min, Fu, Xinyue, Yi, Qinghe, Xu, Enhong, Dong, Longbao. 2024. Impaired mitochondrial oxidative phosphorylation induces CD8+ T cell exhaustion. In Biochemical and biophysical research communications, 734, 150738. doi:10.1016/j.bbrc.2024.150738. https://pubmed.ncbi.nlm.nih.gov/39342799/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen