C57BL/6JCya-Mxra7em1flox/Cya
Common Name:
Mxra7-flox
Product ID:
S-CKO-13791
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mxra7-flox
Strain ID
CKOCMP-67622-Mxra7-B6J-VA
Gene Name
Product ID
S-CKO-13791
Gene Alias
1810057P16Rik; E130302J09Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mxra7em1flox/Cya mice (Catalog S-CKO-13791) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021170
NCBI RefSeq
NM_026280
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Mxra7, Matrix Remodeling Associated 7, is a gene related to matrix remodeling processes. It is involved in various biological functions, potentially through associated signaling pathways such as ERK/MAPK, NF-κB, and others. Its study is crucial as it impacts multiple physiological and pathological processes [1,2,3].
In gene knockout studies, Mxra7-/-mice showed reduced megakaryocytes in the bone marrow and spleen, decreased platelet count and function in peripheral blood. Knock-out inhibited the differentiation of hematopoietic stem/progenitor cells to megakaryocytes by down-regulating GATA-1 and FOG-1 [1]. In acute promyelocytic leukemia cells (NB4), knockdown of Mxra7 promoted drug-induced apoptosis and ATRA-induced cell differentiation, while overexpression had opposite effects. In B-ALL cell line REH, knockdown inhibited cell proliferation and increased sensitivity to cytarabine [3,4]. In SHI-1 cells, overexpression of Mxra7 reduced drug-induced apoptosis by increasing BCL-2 expression [5]. In CCl4-induced acute liver injury in mice, Mxra7 deficiency alleviated liver damage, while overexpression aggravated it. Mxra7-/-mice also showed reduced neutrophil recruitment, CD8+ T-cell number, and cytokine release, along with suppressed MAPK and AKT/NF-κB pathways [6]. In cutaneous wound healing, MXRA7-deficient mice had impaired wound healing, with reduced fibroblast migration, contraction, and proliferation [7]. In psoriatic epidermis, MXRA7-deficient mice developed severer psoriasis-like diseases [8]. In bone marrow mesenchymal stem cells, MXRA7-deficient mice had retarded osteogenesis, and cultured MXRA7-deficient BMSCs showed decreased osteogenesis upon induction [9].
In conclusion, Mxra7 plays essential roles in processes like megakaryocyte differentiation, platelet production, cell apoptosis and differentiation in leukemia, liver injury, wound healing, psoriasis, and bone formation. Gene knockout mouse models have been instrumental in revealing its functions in these disease-related areas, providing potential new targets for treating platelet-related diseases, leukemia, liver injury, psoriasis, and bone-related disorders [1,3,4,5,6,7,8,9].
References：
1. Sun, Zhenjiang, Wang, Benfang, Shen, Ying, Wang, Yiqiang, Lin, Dandan. 2023. MXRA7 is involved in megakaryocyte differentiation and platelet production. In Blood science (Baltimore, Md.), 5, 160-169. doi:10.1097/BS9.0000000000000167. https://pubmed.ncbi.nlm.nih.gov/37546710/
2. Sun, Zhenjiang, Ke, Peng, Shen, Ying, Lin, Dandan, Wang, Yiqiang. 2024. MXRA7 is involved in monocyte-to-macrophage differentiation. In Molecular immunology, 171, 12-21. doi:10.1016/j.molimm.2024.05.001. https://pubmed.ncbi.nlm.nih.gov/38735126/
3. Sun, Zhenjiang, Lin, Dandan, Shen, Ying, Wu, Depei, Wang, Yiqiang. 2023. Critical role of MXRA7 in differentiation blockade in human acute promyelocytic leukemia cells. In Experimental hematology, 125-126, 45-54. doi:10.1016/j.exphem.2023.07.001. https://pubmed.ncbi.nlm.nih.gov/37419299/
4. Ma, Kun-Peng, Sun, Zhen-Jiang, Shen, Ying, Wang, Yi-Qiang, Lin, Dan-Dan. . [Effect of MXRA7 on the Biological Functions of Acute B Lymphoblastic Leukemia Cell Line REH]. In Zhongguo shi yan xue ye xue za zhi, 31, 50-56. doi:10.19746/j.cnki.issn.1009-2137.2023.01.008. https://pubmed.ncbi.nlm.nih.gov/36765476/
5. Zheng, Yu-Dan, Sun, Zheng-Jiang, Ma, Kun-Peng, Wang, Yi-Qiang, Lin, Dan-Dan. . [The Effect of Matrix Remodeling Associated 7 (MXRA7) Expression on the Biological Function of SHI-1 Cells]. In Zhongguo shi yan xue ye xue za zhi, 30, 688-694. doi:10.19746/j.cnki.issn.1009-2137.2022.03.005. https://pubmed.ncbi.nlm.nih.gov/35680791/
6. Lin, Dandan, Sun, Zhenjiang, Jin, Ziqi, Shen, Ying, Wang, Yiqiang. 2018. Matrix Remodeling Associated 7 Deficiency Alleviates Carbon Tetrachloride-Induced Acute Liver Injury in Mice. In Frontiers in immunology, 9, 773. doi:10.3389/fimmu.2018.00773. https://pubmed.ncbi.nlm.nih.gov/29720975/
7. Shen, Ying, Ning, Jinling, Zhao, Lu, Yu, Jie, Wang, Yiqiang. 2023. Matrix remodeling associated 7 proteins promote cutaneous wound healing through vimentin in coordinating fibroblast functions. In Inflammation and regeneration, 43, 5. doi:10.1186/s41232-023-00256-8. https://pubmed.ncbi.nlm.nih.gov/36647132/
8. Ning, Jinling, Shen, Ying, Wang, Ting, Chen, Lingling, Wang, Yiqiang. . Altered expression of matrix remodelling associated 7 (MXRA7) in psoriatic epidermis: Evidence for a protective role in the psoriasis imiquimod mouse model. In Experimental dermatology, 27, 1038-1042. doi:10.1111/exd.13687. https://pubmed.ncbi.nlm.nih.gov/29781547/
9. Zhou, Zhishuai, Shen, Ying, Yin, Juanjuan, Chen, Jianquan, Wang, Yiqiang. 2019. Matrix remodeling associated 7 promotes differentiation of bone marrow mesenchymal stem cells toward osteoblasts. In Journal of cellular physiology, 234, 18053-18064. doi:10.1002/jcp.28438. https://pubmed.ncbi.nlm.nih.gov/30843215/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen