C57BL/6JCya-Lipfem1flox/Cya
Common Name:
Lipf-flox
Product ID:
S-CKO-13837
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Lipf-flox
Strain ID
CKOCMP-67717-Lipf-B6J-VA
Gene Name
Product ID
S-CKO-13837
Gene Alias
2310051B21Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lipfem1flox/Cya mice (Catalog S-CKO-13837) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025680
NCBI RefSeq
NM_026334
Target Region
Exon 4
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
LipF, also known as gastric lipase, is associated with various lipid metabolism processes. In gastric adenocarcinoma, it has been implicated in cell differentiation and prognosis-related pathways [2,3].
In Mycobacterium tuberculosis, reduced LipF expression may contribute to the development of rifampicin (RIF) and streptomycin (STR) resistance. Expressing lipF from Mycobacterium tuberculosis in Mycobacterium smegmatis reduced the minimum inhibitory concentration of RIF and STR, and the growth of M. smegmatis was reduced in the presence of these drugs compared to a control strain without LipF expression [1].
In gastric adenocarcinoma, a subgroup expressing chief-cell markers including LIPF was consistent with the fundic gland-type GA (chief cell-predominant, GA-FG-CCP). Also, decreased LIPF expression in gastric cancer was correlated with DGKA and predicted a poor outcome [2,3].
In conclusion, LipF is involved in lipid metabolism and has significant implications in mycobacterial drug resistance and gastric cancer prognosis. The findings from these studies help in understanding the biological functions of LipF in disease-related processes, providing insights for further research and potential treatment strategies in tuberculosis and gastric cancer.
References：
1. Arriaga-Guerrero, Ana Leticia, Hernández-Luna, Carlos E, Rigal-Leal, Joyce, Bermúdez de León, Mario, Peñuelas-Urquides, Katia. 2020. LipF increases rifampicin and streptomycin sensitivity in a Mycobacterium tuberculosis surrogate. In BMC microbiology, 20, 132. doi:10.1186/s12866-020-01802-x. https://pubmed.ncbi.nlm.nih.gov/32450809/
2. Zhang, Min, Hu, Shuofeng, Min, Min, Ying, Xiaomin, Liu, Yan. 2020. Dissecting transcriptional heterogeneity in primary gastric adenocarcinoma by single cell RNA sequencing. In Gut, 70, 464-475. doi:10.1136/gutjnl-2019-320368. https://pubmed.ncbi.nlm.nih.gov/32532891/
3. Kong, Yi, Zheng, Yan, Jia, Yanfei, Li, Ping, Wang, Yunshan. 2016. Decreased LIPF expression is correlated with DGKA and predicts poor outcome of gastric cancer. In Oncology reports, 36, 1852-60. doi:10.3892/or.2016.4989. https://pubmed.ncbi.nlm.nih.gov/27498782/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen