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C57BL/6JCya-Chd8em1flox/Cya
Common Name:
Chd8-flox
Product ID:
S-CKO-13870
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Chd8-flox
Strain ID
CKOCMP-67772-Chd8-B6J-VA
Gene Name
Chd8
Product ID
S-CKO-13870
Gene Alias
5830451P18Rik; Chd-8; Duplin; HELSNF1; mKIAA1564
Background
C57BL/6JCya
NCBI ID
67772
Modification
Conditional knockout
Chromosome
14
Phenotype
MGI:1915022
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Chd8em1flox/Cya mice (Catalog S-CKO-13870) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000200169
NCBI RefSeq
NM_201637
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Chd8, or Chromodomain Helicase DNA-binding 8, is a transcriptional regulator expressed in nearly all cell types. It is involved in multiple cellular processes such as cell cycle, cell adhesion, neuronal development, myelination, and synaptogenesis [3]. Mutations in Chd8 are strongly associated with autism spectrum disorder (ASD), and its study via genetic models can help understand the underlying mechanisms.

CHD8 haploinsufficiency in human cerebral organoids disrupts neurodevelopmental trajectories, accelerating inhibitory neuron generation and delaying excitatory neuron generation, which may be related to patients' macrocephaly [1]. Disruptive CHD8 mutations in humans define a subtype of autism, and zebrafish with chd8 disruption recapitulate features like increased head size and gastrointestinal motility impairment [2]. CHD8 suppression in induced pluripotent stem cell-derived neural progenitors impacts histone H3K36me3 and RNA alternative splicing [4]. Adult ablation of Chd8 in astrocytes in mice attenuates reactive gliosis and neuroinflammation [5].

In conclusion, Chd8 is crucial for normal neurodevelopment. Its disruption, as studied in various models, is strongly linked to ASD and other related phenotypes such as macrocephaly, and may also be involved in processes like reactive gliosis and neuroinflammation. Understanding Chd8's function through these models provides insights into the mechanisms of neurodevelopmental diseases.

References:
1. Villa, Carlo Emanuele, Cheroni, Cristina, Dotter, Christoph P, Testa, Giuseppe, Novarino, Gaia. . CHD8 haploinsufficiency links autism to transient alterations in excitatory and inhibitory trajectories. In Cell reports, 39, 110615. doi:10.1016/j.celrep.2022.110615. https://pubmed.ncbi.nlm.nih.gov/35385734/
2. Bernier, Raphael, Golzio, Christelle, Xiong, Bo, Katsanis, Nicholas, Eichler, Evan E. 2014. Disruptive CHD8 mutations define a subtype of autism early in development. In Cell, 158, 263-276. doi:10.1016/j.cell.2014.06.017. https://pubmed.ncbi.nlm.nih.gov/24998929/
3. Weissberg, Orly, Elliott, Evan. 2021. The Mechanisms of CHD8 in Neurodevelopment and Autism Spectrum Disorders. In Genes, 12, . doi:10.3390/genes12081133. https://pubmed.ncbi.nlm.nih.gov/34440307/
4. Kerschbamer, Emanuela, Arnoldi, Michele, Tripathi, Takshashila, Ferrari, Francesco, Biagioli, Marta. . CHD8 suppression impacts on histone H3 lysine 36 trimethylation and alters RNA alternative splicing. In Nucleic acids research, 50, 12809-12828. doi:10.1093/nar/gkac1134. https://pubmed.ncbi.nlm.nih.gov/36537238/
5. Megagiannis, Platon, Mei, Yuan, Yan, Rachel E, Sanjana, Neville E, Zhou, Yang. 2024. Autism-associated CHD8 controls reactive gliosis and neuroinflammation via remodeling chromatin in astrocytes. In Cell reports, 43, 114637. doi:10.1016/j.celrep.2024.114637. https://pubmed.ncbi.nlm.nih.gov/39154337/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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