C57BL/6JCya-Rnf115em1flox/Cya
Common Name:
Rnf115-flox
Product ID:
S-CKO-13908
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Rnf115-flox
Strain ID
CKOCMP-67845-Rnf115-B6J-VA
Gene Name
Product ID
S-CKO-13908
Gene Alias
2610028E05Rik; Zfp364
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rnf115em1flox/Cya mice (Catalog S-CKO-13908) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029740
NCBI RefSeq
NM_026406
Target Region
Exon 4
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Rnf115, also known as breast cancer-associated gene 2 (BCA2), is an E3 ubiquitin ligase. Ubiquitin ligases play crucial roles in regulating protein degradation, cellular signaling, and immune responses. Rnf115 is involved in pathways such as autophagy, immune regulation, and cell-cycle control, which are vital for maintaining cellular homeostasis and organismal health. Genetic models, like knockout (KO) mouse models, have been instrumental in studying its functions [1-10].
In KO mouse models, Rnf115 deletion alleviated acute liver injury by promoting autophagy and inhibiting the inflammatory response [1]. It also inhibited M1 type macrophage activation via NF-κB and Jnk signaling pathways. In Trex1-/-mice and STINGN153S/WT bone marrow chimeric mice, knockout of RNF115 significantly inhibited systemic inflammation and autoimmune lethality [2]. Additionally, in Rnf115-/-cells, there were changes in the trafficking of Toll-like receptors (TLRs), innate antiviral responses, and autophagosome maturation [3,4,6]. In cancer-related studies, Rnf115 deficiency inhibited the growth of gastric cancer, hepatocellular carcinoma, and lung adenocarcinoma cells, while its overexpression in thyroid carcinoma and lung adenocarcinoma promoted tumor progression [5,6,7,8,9].
In conclusion, Rnf115 plays diverse and significant roles in biological processes such as autophagy, immune regulation, and tumorigenesis. The use of Rnf115 KO mouse models has provided valuable insights into its functions in various disease areas, including liver injury, autoimmune diseases, and different types of cancers, helping to further our understanding of disease mechanisms and potentially guiding the development of new therapeutic strategies.
References:
1. Feng, Jinqiu, Ye, Shufang, Hai, Bao, Lu, Wenping, Chen, Yingyu. 2023. RNF115/BCA2 deficiency alleviated acute liver injury in mice by promoting autophagy and inhibiting inflammatory response. In Cell death & disease, 14, 855. doi:10.1038/s41419-023-06379-7. https://pubmed.ncbi.nlm.nih.gov/38129372/
2. Zhang, Zhi-Dong, Shi, Chang-Rui, Li, Fang-Xu, Zhong, Bo, Lin, Dandan. 2024. Disulfiram ameliorates STING/MITA-dependent inflammation and autoimmunity by targeting RNF115. In Cellular & molecular immunology, 21, 275-291. doi:10.1038/s41423-024-01131-3. https://pubmed.ncbi.nlm.nih.gov/38267694/
3. Zhang, Zhi-Dong, Li, Hong-Xu, Gan, Hu, Zhong, Bo, Lin, Dandan. 2022. RNF115 Inhibits the Post-ER Trafficking of TLRs and TLRs-Mediated Immune Responses by Catalyzing K11-Linked Ubiquitination of RAB1A and RAB13. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9, e2105391. doi:10.1002/advs.202105391. https://pubmed.ncbi.nlm.nih.gov/35343654/
4. Zhang, Zhi-Dong, Xiong, Tian-Chen, Yao, Shu-Qi, Lin, Dandan, Zhong, Bo. 2020. RNF115 plays dual roles in innate antiviral responses by catalyzing distinct ubiquitination of MAVS and MITA. In Nature communications, 11, 5536. doi:10.1038/s41467-020-19318-3. https://pubmed.ncbi.nlm.nih.gov/33139700/
5. Zhu, Jinxiang, Guo, Longwei, Dai, Hao, Zhao, Qian, Xu, Chongwen. 2024. RNF115 aggravates tumor progression through regulation of CDK10 degradation in thyroid carcinoma. In Cell biology and toxicology, 40, 14. doi:10.1007/s10565-024-09845-w. https://pubmed.ncbi.nlm.nih.gov/38376606/
6. Li, Riyong, Gu, Zhaohui, Zhang, Xuan, Lv, Ping, Chen, Yingyu. 2020. RNF115 deletion inhibits autophagosome maturation and growth of gastric cancer. In Cell death & disease, 11, 810. doi:10.1038/s41419-020-03011-w. https://pubmed.ncbi.nlm.nih.gov/32980859/
7. Wu, Xiao-Ting, Wang, Yu-Han, Cai, Xiao-Yue, Lu, Wen-Feng, Zhang, Ming. 2021. RNF115 promotes lung adenocarcinoma through Wnt/β-catenin pathway activation by mediating APC ubiquitination. In Cancer & metabolism, 9, 7. doi:10.1186/s40170-021-00243-y. https://pubmed.ncbi.nlm.nih.gov/33509267/
8. Luo, Zhigang, Ye, Xin, Shou, Feng, Li, Fugang, Wang, Gang. 2020. RNF115-mediated ubiquitination of p53 regulates lung adenocarcinoma proliferation. In Biochemical and biophysical research communications, 530, 425-431. doi:10.1016/j.bbrc.2020.05.061. https://pubmed.ncbi.nlm.nih.gov/32553631/
9. Gu, Zhaohui, Feng, Jinqiu, Ye, Shufang, Zhu, Bin, Chen, Yingyu. 2025. RNF115 deficiency upregulates autophagy and inhibits hepatocellular carcinoma growth. In Chinese medical journal, 138, 754-756. doi:10.1097/CM9.0000000000003466. https://pubmed.ncbi.nlm.nih.gov/39932173/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen