C57BL/6JCya-Camsap2em1flox/Cya
Common Name
Camsap2-flox
Product ID
S-CKO-13940
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-67886-Camsap2-B6J-VA
Status
When using this mouse strain in a publication, please cite “Camsap2-flox Mouse (Catalog S-CKO-13940) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Camsap2-flox
Strain ID
CKOCMP-67886-Camsap2-B6J-VA
Gene Name
Product ID
S-CKO-13940
Gene Alias
Camsap1l1, mKIAA1078, 1600013L13Rik, 4930541M15Rik
Background
C57BL/6JCya
Gene Full Name
calmodulin regulated spectrin-associated protein family, member 2
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 1 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000192314
NCBI Transcript ID
NM_001347109
Target Region
Exon 2
Size of Effective Region
~1.3 kb
Overview of Gene Research
Camsap2, short for calmodulin-regulated spectrin-associated protein 2, is a microtubule minus-end-targeting protein. It plays a crucial role in microtubule organization, participating in processes like microtubule nucleation, stabilization, and release from the γ-tubulin ring complex (γ-TuRC). It is involved in multiple signaling pathways such as Trio/Rac1, JNK/c-Jun, and TGF-β, and is of great biological importance in cell migration, polarity, and intracellular transport [2,3,6,7].
In hepatocellular carcinoma, Camsap2 is upregulated and drives metastasis by mediating noncentrosomal microtubule acetylation. Depletion of Camsap2 transforms the noncentrosomal microtubule array into a radial centrosomal pattern, impairing cell migration and invasion [1]. In colorectal cancer, high Camsap2 expression promotes cell migration, invasion, and metastasis through activation of the JNK/c-Jun/MMP-1 signaling pathway. Silencing Camsap2 leads to the opposite effects [3]. In lung cancer, Camsap2 enhances metastasis by mediating RASAL2 degradation, and its knockdown inhibits cell motility in vitro and metastasis in vivo [4]. In gastric cancer, high Camsap2 expression promotes invasion and metastasis possibly by upregulating TGF-β signaling to promote epithelial-mesenchymal transition (EMT) [6]. In Sertoli cells, Camsap2 knockdown promotes tight junction barrier function and blocks perfluorooctanesulfonate (PFOS)-induced cell injury by regulating microtubule and actin dynamics [5].
In conclusion, Camsap2 is essential for microtubule-related cellular functions. Gene knockout or knockdown models of Camsap2 in various cancer types and cell systems have revealed its oncogenic role in promoting cancer cell migration, invasion, and metastasis. These findings suggest that targeting Camsap2-mediated pathways could be a potential therapeutic strategy for treating related cancers [1,3,4,6].
References：
1. Li, Dongxiao, Ding, Xiangming, Xie, Meng, Tian, Dean, Xia, Limin. 2020. CAMSAP2-mediated noncentrosomal microtubule acetylation drives hepatocellular carcinoma metastasis. In Theranostics, 10, 3749-3766. doi:10.7150/thno.42596. https://pubmed.ncbi.nlm.nih.gov/32206120/
2. Imasaki, Tsuyoshi, Kikkawa, Satoshi, Niwa, Shinsuke, Takeichi, Masatoshi, Nitta, Ryo. 2022. CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation. In eLife, 11, . doi:10.7554/eLife.77365. https://pubmed.ncbi.nlm.nih.gov/35762204/
3. Wang, Xiaojuan, Liu, Yumin, Ding, Yawen, Feng, Gang. 2022. CAMSAP2 promotes colorectal cancer cell migration and invasion through activation of JNK/c-Jun/MMP-1 signaling pathway. In Scientific reports, 12, 16899. doi:10.1038/s41598-022-21345-7. https://pubmed.ncbi.nlm.nih.gov/36207462/
4. Singharajkomron, Natsaranyatron, Yodsurang, Varalee, Limprasutr, Vudhiporn, Hayakawa, Yoshihiro, Pongrakhananon, Varisa. 2023. CAMSAP2 enhances lung cancer cell metastasis by mediating RASAL2 degradation. In Life sciences, 338, 122391. doi:10.1016/j.lfs.2023.122391. https://pubmed.ncbi.nlm.nih.gov/38159595/
5. Mao, Bai-Ping, Li, Linxi, Ge, Renshan, Lian, Qingquan, Cheng, C Yan. . CAMSAP2 Is a Microtubule Minus-End Targeting Protein That Regulates BTB Dynamics Through Cytoskeletal Organization. In Endocrinology, 160, 1448-1467. doi:10.1210/en.2018-01097. https://pubmed.ncbi.nlm.nih.gov/30994903/
6. Zuo, L, Wang, L, Yang, Z, Zhnag, X, Geng, Z. . [High expression of CAMSAP2 promotes invasion and metastasis of gastric cancer cells by upregulating TGF-β signaling]. In Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 43, 1460-1468. doi:10.12122/j.issn.1673-4254.2023.09.02. https://pubmed.ncbi.nlm.nih.gov/37814859/
7. Rai, Dipti, Song, Yinlong, Hua, Shasha, Jiang, Kai, Akhmanova, Anna. 2024. CAMSAPs and nucleation-promoting factors control microtubule release from γ-TuRC. In Nature cell biology, 26, 404-420. doi:10.1038/s41556-024-01366-2. https://pubmed.ncbi.nlm.nih.gov/38424271/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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