C57BL/6JCya-Slc25a39em1flox/Cya
Common Name:
Slc25a39-flox
Product ID:
S-CKO-14040
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc25a39-flox
Strain ID
CKOCMP-68066-Slc25a39-B6J-VA
Gene Name
Product ID
S-CKO-14040
Gene Alias
3010027G13Rik; D11Ertd333e
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc25a39em1flox/Cya mice (Catalog S-CKO-14040) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000018821
NCBI RefSeq
NM_026542
Target Region
Exon 6~12
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Slc25a39 is a mitochondrial membrane carrier. It is crucial for mitochondrial glutathione (GSH) import, a process essential for maintaining redox homeostasis in mitochondria [1,2,3]. As mitochondria are the major sites of oxidative reactions, GSH imported by Slc25a39 performs protective and biosynthetic functions. This process is also linked to iron metabolism and is involved in cell proliferation in vitro and red blood cell development in mice [1].
Loss-of-function studies, such as knocking out Slc25a39 in mammalian cells, have shown that its absence reduces mitochondrial GSH import and abundance without affecting cellular GSH levels [1]. Cells lacking both Slc25a39 and its paralogue Slc25a40 exhibit defects in the activity and stability of proteins containing iron-sulfur clusters [1]. In mice, Slc25a39 expression changes in response to conditions like bile duct ligation and lipopolysaccharide treatment, which are associated with changes in mitochondrial GSH levels [4]. In addition, in colorectal cancer, knockdown of Slc25a39 suppresses cell growth and migration while enhancing apoptosis, indicating its role in cancer progression [5].
In summary, Slc25a39 is essential for mitochondrial GSH import, which impacts multiple biological processes. Model-based research, especially gene knockout studies in mammalian cells and mice, has revealed its significance in redox homeostasis, iron metabolism, and disease-related processes such as cancer progression. Understanding Slc25a39 may offer new insights into treating conditions related to mitochondrial dysfunction and redox imbalance.
References:
1. Wang, Ying, Yen, Frederick S, Zhu, Xiphias Ge, Hite, Richard, Birsoy, Kıvanç. 2021. SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells. In Nature, 599, 136-140. doi:10.1038/s41586-021-04025-w. https://pubmed.ncbi.nlm.nih.gov/34707288/
2. Shi, Xiaojian, Reinstadler, Bryn, Shah, Hardik, Mootha, Vamsi K, Shen, Hongying. 2022. Combinatorial GxGxE CRISPR screen identifies SLC25A39 in mitochondrial glutathione transport linking iron homeostasis to OXPHOS. In Nature communications, 13, 2483. doi:10.1038/s41467-022-30126-9. https://pubmed.ncbi.nlm.nih.gov/35513392/
3. Liu, Yuyang, Liu, Shanshan, Tomar, Anju, Mansy, Sheref S, Birsoy, Kıvanç. 2023. Autoregulatory control of mitochondrial glutathione homeostasis. In Science (New York, N.Y.), 382, 820-828. doi:10.1126/science.adf4154. https://pubmed.ncbi.nlm.nih.gov/37917749/
4. Kawase, Atsushi, Hatanaka, Momoko, Matsuda, Naoya, Shimada, Hiroaki, Iwaki, Masahiro. 2022. Slc25a39 and Slc25a40 Expression in Mice with Bile Duct Ligation or Lipopolysaccharide Treatment. In International journal of molecular sciences, 23, . doi:10.3390/ijms23158573. https://pubmed.ncbi.nlm.nih.gov/35955707/
5. Zhang, Wentao, Ou, Zhigao, Tang, Ting, Niu, Li, Zhu, Jianjun. 2024. Up-regulated SLC25A39 promotes cell growth and metastasis via regulating ROS production in colorectal cancer. In Journal of Cancer, 15, 5841-5854. doi:10.7150/jca.98844. https://pubmed.ncbi.nlm.nih.gov/39308681/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen