C57BL/6JCya-Gar1em1flox/Cya
Common Name:
Gar1-flox
Product ID:
S-CKO-14074
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gar1-flox
Strain ID
CKOCMP-68147-Gar1-B6J-VA
Gene Name
Product ID
S-CKO-14074
Gene Alias
C430047J18Rik; Nola1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gar1em1flox/Cya mice (Catalog S-CKO-14074) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029643
NCBI RefSeq
NM_026578
Target Region
Exon 3~4
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Gar1 is an essential small nucleolar ribonucleoprotein (RNP) protein. It contains two GAR domains rich in glycine and arginine residues [2]. Gar1 is localized in the yeast nucleolus and is crucial for cell growth, being involved in pre-rRNA processing as depletion of it impairs the processing of the 35S primary transcript of pre-rRNA and prevents 18S rRNA synthesis [2]. It is also part of the box H/ACA ribonucleoprotein (RNP) complex, which is involved in post-transcriptional RNA pseudouridylation [4].
In the context of the H/ACA RNP complex, Gar1 has several functions. It has a novel SUMO-interacting motif that mediates the interaction between dyskerin and GAR1, and dyskerin mislocalization due to disruption of this interaction can lead to reduced interaction with telomerase RNA [1]. Gar1 reduces the catalytic barrier in H/ACA RNA-guided pseudouridylation by affecting the activation entropy, helps place the target uridine correctly for the pseudouridylation reaction, and is involved in product release [3]. In archaea, Gar1 increases the catalytic activity of Cbf5 in pseudouridylating tRNA, enhances Cbf5's affinity for tRNA, and is crucial for in vivo and in vitro RNA guide-independent formation of Ψ2607 by Cbf5 [5,6].
In conclusion, Gar1 is vital for ribosome biogenesis through its role in pre-rRNA processing and for post-transcriptional RNA modification, specifically pseudouridylation. Its functions within the H/ACA RNP complex, especially in relation to dyskerin and Cbf5, highlight its importance in these biological processes. Although no KO/CKO mouse model-specific findings were in the provided references, understanding Gar1's functions provides a basis for further in vivo studies, which could potentially uncover its role in disease-related pathways.
References:
1. MacNeil, D E, Lambert-Lanteigne, P, Qin, J, Thibault, P, Autexier, C. 2021. SUMOylation- and GAR1-Dependent Regulation of Dyskerin Nuclear and Subnuclear Localization. In Molecular and cellular biology, 41, . doi:10.1128/MCB.00464-20. https://pubmed.ncbi.nlm.nih.gov/33526451/
2. Girard, J P, Lehtonen, H, Caizergues-Ferrer, M, Tollervey, D, Lapeyre, B. . GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast. In The EMBO journal, 11, 673-82. doi:. https://pubmed.ncbi.nlm.nih.gov/1531632/
3. Wang, Peng, Yang, Lijiang, Gao, Yi Qin, Zhao, Xin Sheng. 2015. Accurate placement of substrate RNA by Gar1 in H/ACA RNA-guided pseudouridylation. In Nucleic acids research, 43, 7207-16. doi:10.1093/nar/gkv757. https://pubmed.ncbi.nlm.nih.gov/26206671/
4. De Zoysa, Meemanage D, Yu, Yi-Tao. 2017. Posttranscriptional RNA Pseudouridylation. In The Enzymes, 41, 151-167. doi:10.1016/bs.enz.2017.02.001. https://pubmed.ncbi.nlm.nih.gov/28601221/
5. Kamalampeta, Rajashekhar, Kothe, Ute. 2012. Archaeal proteins Nop10 and Gar1 increase the catalytic activity of Cbf5 in pseudouridylating tRNA. In Scientific reports, 2, 663. doi:. https://pubmed.ncbi.nlm.nih.gov/22993689/
6. Fujikane, Ryosuke, Behm-Ansmant, Isabelle, Tillault, Anne-Sophie, Motorin, Yuri, Charpentier, Bruno. 2018. Contribution of protein Gar1 to the RNA-guided and RNA-independent rRNA:Ψ-synthase activities of the archaeal Cbf5 protein. In Scientific reports, 8, 13815. doi:10.1038/s41598-018-32164-0. https://pubmed.ncbi.nlm.nih.gov/30218085/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen