C57BL/6JCya-Tmed10em1flox/Cya
Common Name:
Tmed10-flox
Product ID:
S-CKO-14225
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tmed10-flox
Strain ID
CKOCMP-68581-Tmed10-B6J-VA
Gene Name
Product ID
S-CKO-14225
Gene Alias
1110014C03Rik; Tmp21; p23; p24delta1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmed10em1flox/Cya mice (Catalog S-CKO-14225) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040766
NCBI RefSeq
NM_026775
Target Region
Exon 2
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
TMED10, the transmembrane p24 trafficking protein 10, is a conserved vesicle trafficking protein [3]. It is involved in multiple biological processes, especially unconventional protein secretion (UcPS), where it functions as a protein channel for the vesicle entry and secretion of leaderless cargoes [1]. This process is crucial for the secretion of various proteins that lack signal peptides, and TMED10 also participates in pathways related to lipid nanodomain formation at the plasma membrane [4].
In zebrafish larvae, Tmed10 deficiency leads to impaired exocrine pancreatic differentiation. Tmed10 regulates this process through γ -secretase, as reduced Tmed10 results in decreased membrane-bound β-catenin and hyperactivation of β-catenin signaling, causing exocrine pancreas defects [3]. In mouse myoblast C2C12 cells, TMED10 is essential for the secretion of insulin-like growth factor 2 (IGF2) and myoblast differentiation. It directly regulates ER export and indirectly regulates TGN export of IGF2 [2].
In conclusion, TMED10 plays a vital role in multiple biological processes, including protein secretion and cell differentiation. The study of Tmed10 knockout models, such as in zebrafish and mouse cells, has provided insights into its role in pancreatic development and myoblast differentiation, suggesting its potential significance in related diseases [2,3].
References：
1. Zhang, Min, Liu, Lei, Lin, Xubo, Zheng, Li, Ge, Liang. 2020. A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion. In Cell, 181, 637-652.e15. doi:10.1016/j.cell.2020.03.031. https://pubmed.ncbi.nlm.nih.gov/32272059/
2. Li, Tiantian, Yang, Feng, Heng, Youshan, Wu, Zhenguo, Guo, Yusong. 2023. TMED10 mediates the trafficking of insulin-like growth factor 2 along the secretory pathway for myoblast differentiation. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2215285120. doi:10.1073/pnas.2215285120. https://pubmed.ncbi.nlm.nih.gov/37931110/
3. Tao, Zewen, Yang, Di, Ni, Rui. 2023. Tmed10 deficiency results in impaired exocrine pancreatic differentiation in zebrafish larvae. In Developmental biology, 503, 43-52. doi:10.1016/j.ydbio.2023.08.003. https://pubmed.ncbi.nlm.nih.gov/37597605/
4. Anwar, Muhammad U, Sergeeva, Oksana A, Abrami, Laurence, D'Angelo, Giovanni, van der Goot, F Gisou. 2022. ER-Golgi-localized proteins TMED2 and TMED10 control the formation of plasma membrane lipid nanodomains. In Developmental cell, 57, 2334-2346.e8. doi:10.1016/j.devcel.2022.09.004. https://pubmed.ncbi.nlm.nih.gov/36174556/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen