C57BL/6JCya-Myct1em1flox/Cya
Common Name:
Myct1-flox
Product ID:
S-CKO-14242
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Myct1-flox
Strain ID
CKOCMP-68632-Myct1-B6J-VA
Gene Name
Product ID
S-CKO-14242
Gene Alias
1110020B04Rik; Mtmc1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Myct1em1flox/Cya mice (Catalog S-CKO-14242) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000051809
NCBI RefSeq
NM_026793
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
MYCT1, also known as MTLC, is a crucial gene with diverse functions. As a novel c-Myc target gene, it is regulated by multiple transcription factors. It plays essential roles in various biological processes such as cell proliferation, apoptosis, migration, and differentiation, and is involved in tumor angiogenesis and immune response modulation within the tumor microenvironment [2].
In human haematopoietic stem cells (HSCs), lentivirus-mediated knockdown of MYCT1 prevented HSPC expansion and engraftment, while restoring its expression improved these processes. Single-cell RNA sequencing showed that MYCT1 governs regulatory programmes and cellular properties for HSC stemness, and its loss led to excessive endocytosis and hyperactive signalling [1].
In mice, global inactivation of Myct1 led to progressive glycogen accumulation in the liver, as MYCT1 promoted the translation efficiency of enzymes related to the glycogen shunt in a RACK1-dependent manner [3]. In diffuse large B-cell lymphoma, MYCT1 inhibited hematopoiesis by suppressing RUNX1 transcription [4].
In conclusion, MYCT1 is a multi-functional gene. Its study through gene-knockout models has revealed its significance in areas such as haematopoiesis, metabolism, and cancer development. These findings provide valuable insights into understanding biological processes and developing potential therapeutic strategies for related diseases.
References:
1. Aguadé-Gorgorió, Júlia, Jami-Alahmadi, Yasaman, Calvanese, Vincenzo, Wohlschlegel, James A, Mikkola, Hanna K A. 2024. MYCT1 controls environmental sensing in human haematopoietic stem cells. In Nature, 630, 412-420. doi:10.1038/s41586-024-07478-x. https://pubmed.ncbi.nlm.nih.gov/38839950/
2. Xu, Jianan, Sun, Yuanyuan, Fu, Weineng, Fu, Shuang. 2023. MYCT1 in cancer development: Gene structure, regulation, and biological implications for diagnosis and treatment. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 165, 115208. doi:10.1016/j.biopha.2023.115208. https://pubmed.ncbi.nlm.nih.gov/37499454/
3. Ding, Dong-Xue, Wang, Yue, Yan, Wei, Fu, Wei-Neng. 2022. MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes. In iScience, 25, 103955. doi:10.1016/j.isci.2022.103955. https://pubmed.ncbi.nlm.nih.gov/35281731/
4. Liang, Ying, Wei, Xin, Yue, Peng-Jie, Sun, Yuan-Yuan, Fu, Wei-Neng. 2024. MYCT1 inhibits hematopoiesis in diffuse large B-cell lymphoma by suppressing RUNX1 transcription. In Cellular & molecular biology letters, 29, 5. doi:10.1186/s11658-023-00522-0. https://pubmed.ncbi.nlm.nih.gov/38172714/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen