C57BL/6JCya-Pcyt2em1flox/Cya
Common Name:
Pcyt2-flox
Product ID:
S-CKO-14256
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Pcyt2-flox
Strain ID
CKOCMP-68671-Pcyt2-B6J-VA
Gene Name
Product ID
S-CKO-14256
Gene Alias
1110033E03Rik; ET
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pcyt2em1flox/Cya mice (Catalog S-CKO-14256) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026129
NCBI RefSeq
NM_024229
Target Region
Exon 2~3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Pcyt2, also known as phosphatidylethanolamine cytidyltransferase (ECT), is a key enzyme in the CDP-ethanolamine pathway for phosphatidylethanolamine synthesis. Phosphatidylethanolamine is an abundant membrane lipid, playing crucial roles in cell division, signaling, activation, autophagy, and phagocytosis [6]. Genetic models like knockout mice are valuable for studying its functions.
Pcyt2 deficiency in humans leads to severe diseases, and pcyt2-mutant zebrafish and muscle-specific Pcyt2-knockout mice mimic the phenotypes, showing failure to thrive, progressive muscle weakness, and accelerated ageing. Mechanistically, it affects cellular bioenergetics and membrane lipid bilayer in muscles [1]. In colorectal cancer, PCYT2 acts as a metastasis suppressor by increasing YAP1 phosphorylation [2]. In mice, heterozygous deletion of Pcyt2 causes age-dependent nonalcoholic steatohepatitis and insulin resistance, which can be reversed by phosphoethanolamine [3]. In Saarlooswolfdogs, a PCYT2 deficiency variant leads to progressive retinal, central, and peripheral neurodegeneration [4]. Also, genetic deletion of Pcyt2 in activated T cells impairs the differentiation of TFH cells and humoral immune responses [5]. In addition, knockdown of PCYT2 in cultured human cells increases the expression of functionally glycosylated α-dystroglycan [7].
In summary, Pcyt2 is essential for maintaining muscle health, regulating lipid biosynthesis, and is involved in various disease conditions such as muscle dystrophy, cancer, nonalcoholic steatohepatitis, neurodegeneration, and immune-related disorders. The study of Pcyt2 knockout models has significantly advanced our understanding of these disease mechanisms, providing potential therapeutic targets for treatment [1-5, 10].
References:
1. Cikes, Domagoj, Elsayad, Kareem, Sezgin, Erdinc, Banka, Siddharth, Penninger, Josef M. 2023. PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing. In Nature metabolism, 5, 495-515. doi:10.1038/s42255-023-00766-2. https://pubmed.ncbi.nlm.nih.gov/36941451/
2. Zhou, Lian, Zhang, Su, Wang, Lingli, Wen, Linda, Han, Junhong. 2024. PCYT2 inhibits epithelial-mesenchymal transition in colorectal cancer by elevating YAP1 phosphorylation. In JCI insight, 9, . doi:10.1172/jci.insight.178823. https://pubmed.ncbi.nlm.nih.gov/39531326/
3. Grapentine, Sophie, Singh, Rathnesh K, Basu, Poulami, Dolinsky, Vernon W, Bakovic, Marica. 2022. Pcyt2 deficiency causes age-dependant development of nonalcoholic steatohepatitis and insulin resistance that could be attenuated with phosphoethanolamine. In Scientific reports, 12, 1048. doi:10.1038/s41598-022-05140-y. https://pubmed.ncbi.nlm.nih.gov/35058529/
4. Christen, Matthias, Oevermann, Anna, Rupp, Stefan, Lohi, Hannes, Leeb, Tosso. 2024. PCYT2 deficiency in Saarlooswolfdogs with progressive retinal, central, and peripheral neurodegeneration. In Molecular genetics and metabolism, 141, 108149. doi:10.1016/j.ymgme.2024.108149. https://pubmed.ncbi.nlm.nih.gov/38277988/
5. Fu, Guotong, Guy, Clifford S, Chapman, Nicole M, Jackowski, Suzanne, Chi, Hongbo. 2021. Metabolic control of TFH cells and humoral immunity by phosphatidylethanolamine. In Nature, 595, 724-729. doi:10.1038/s41586-021-03692-z. https://pubmed.ncbi.nlm.nih.gov/34234346/
6. Bakovic, Marica, Fullerton, Morgan D, Michel, Vera. . Metabolic and molecular aspects of ethanolamine phospholipid biosynthesis: the role of CTP:phosphoethanolamine cytidylyltransferase (Pcyt2). In Biochemistry and cell biology = Biochimie et biologie cellulaire, 85, 283-300. doi:. https://pubmed.ncbi.nlm.nih.gov/17612623/
7. Imae, Rieko, Manya, Hiroshi, Tsumoto, Hiroki, Miura, Yuri, Endo, Tamao. . PCYT2 synthesizes CDP-glycerol in mammals and reduced PCYT2 enhances the expression of functionally glycosylated α-dystroglycan. In Journal of biochemistry, 170, 183-194. doi:10.1093/jb/mvab069. https://pubmed.ncbi.nlm.nih.gov/34255834/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen