C57BL/6JCya-Slc44a2em1flox/Cya
Common Name:
Slc44a2-flox
Product ID:
S-CKO-14260
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc44a2-flox
Strain ID
CKOCMP-68682-Slc44a2-B6J-VA
Gene Name
Product ID
S-CKO-14260
Gene Alias
1110028E10Rik; CTL2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc44a2em1flox/Cya mice (Catalog S-CKO-14260) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034697
NCBI RefSeq
NM_152808
Target Region
Exon 3~10
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Slc44a2, encoding the choline transporter-like protein CTL2, is involved in multiple biological functions. It plays a role in platelet aggregation, neutrophil activation, and is also associated with the regulation of mitochondrial function through choline transport into mitochondria [3,5]. It has been linked to various pathways including those related to thrombosis and vascular-related processes. Genetic models such as knockout mice are valuable for studying its functions.
In aortic aneurysm, VSMC-specific Slc44a2-knockout mice are more susceptible to aortic aneurysm under Ang II infusion, indicating that Slc44a2 is protective. Mechanistically, it interacts with NRP1 and ITGB3 to activate the TGF-β/SMAD signaling pathway, promoting contractile gene expression. Inhibition of Slc44a2 facilitates VSMC phenotypic switching to a synthetic state, contributing to the development of aortic aneurysm [1,2].
In venous thrombosis, Slc44a2-deficient mice have a reduced response in the stenosis-driven venous thrombosis model, suggesting its role in the initiation of venous thrombosis, potentially related to platelet-neutrophil interaction [4].
In conclusion, Slc44a2 is crucial for multiple biological processes. Its functions revealed through gene-knockout mouse models have significant implications in diseases like aortic aneurysm and venous thrombosis, highlighting its potential as a therapeutic target for these vascular-related conditions.
References:
1. Song, Tianyu, Zhao, Shuang, Luo, Shanshan, Xie, Liping, Ji, Yong. 2024. SLC44A2 regulates vascular smooth muscle cell phenotypic switching and aortic aneurysm. In The Journal of clinical investigation, 134, . doi:10.1172/JCI173690. https://pubmed.ncbi.nlm.nih.gov/38916960/
2. Xing, Mengen, Chen, Wanqi, Ji, Yachen, Song, Weihong. 2024. SLC44A2-mediated phenotypic switch of vascular smooth muscle cells contributes to aortic aneurysm. In The Journal of clinical investigation, 134, . doi:10.1172/JCI183527. https://pubmed.ncbi.nlm.nih.gov/39145443/
3. Koehl, Bérengère, Vrignaud, Cédric, Mikdar, Mahmoud, Azouzi, Slim, Peyrard, Thierry. 2023. Lack of the human choline transporter-like protein SLC44A2 causes hearing impairment and a rare red blood phenotype. In EMBO molecular medicine, 15, e16320. doi:10.15252/emmm.202216320. https://pubmed.ncbi.nlm.nih.gov/36695047/
4. Tilburg, Julia, Coenen, Daniëlle M, Zirka, Gaia, Maracle, Chrissta X, Thomas, Grace M. 2020. SLC44A2 deficient mice have a reduced response in stenosis but not in hypercoagulability driven venous thrombosis. In Journal of thrombosis and haemostasis : JTH, 18, 1714-1727. doi:10.1111/jth.14835. https://pubmed.ncbi.nlm.nih.gov/32297475/
5. Bennett, J Allen, Mastrangelo, Michael A, Ture, Sara K, Morrell, Craig N, Lowenstein, Charles J. 2020. The choline transporter Slc44a2 controls platelet activation and thrombosis by regulating mitochondrial function. In Nature communications, 11, 3479. doi:10.1038/s41467-020-17254-w. https://pubmed.ncbi.nlm.nih.gov/32661250/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen