C57BL/6JCya-Zfp467em1flox/Cya
Common Name:
Zfp467-flox
Product ID:
S-CKO-14342
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Zfp467-flox
Strain ID
CKOCMP-68910-Zfp467-B6J-VA
Gene Name
Product ID
S-CKO-14342
Gene Alias
1190001I08Rik; EZI; MNCb-3350; Znf467
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfp467em1flox/Cya mice (Catalog S-CKO-14342) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000114561
NCBI RefSeq
NM_020589
Target Region
Exon 5
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Zfp467, also known as EZI, is a zinc-finger transcription factor. It plays a crucial role in the differentiation of mesenchymal progenitor cells, being involved in pathways related to osteogenesis and adipogenesis. It is also associated with the regulation of Wnt signaling, which is important for bone formation [3,4].
Genetic loss-of-function studies, especially in KO and CKO mouse models, have revealed significant insights. Conditional deletion of Zfp467 in osteogenic precursors led to high bone mass in mice, as Prrx1Cre; Zfp467fl/fl mice showed greater osteogenic differentiation similar to Zfp467-/-mice. Loss of Zfp467 increased Pth1r expression, enhancing the osteogenic response to PTH, and also led to higher nuclear translocation of NFκB1 that binds to the P2 promoter of the Pth1r and increases its transcription. In vitro, COBs and BMSCs from Zfp467-/-mice showed more positive ALP and Alizarin Red staining, decreased adipocyte-related markers, and increased Pth1r mRNA and protein levels [1,2].
In conclusion, Zfp467 negatively influences skeletal homeostasis by favoring adipogenesis over osteogenesis. The Zfp467 KO/CKO mouse models have provided valuable insights into its role in bone-related diseases, suggesting that its repression could be a component of PTH-based therapeutic strategies for osteoporosis and other bone-loss conditions [2].
References：
1. Liu, Hanghang, Wada, Akane, Le, Isabella, Baron, Roland, Rosen, Clifford J. 2023. PTH regulates osteogenesis and suppresses adipogenesis through Zfp467 in a feed-forward, PTH1R-cyclic AMP-dependent manner. In eLife, 12, . doi:10.7554/eLife.83345. https://pubmed.ncbi.nlm.nih.gov/37159501/
2. Le, Phuong T, Liu, Hanghang, Alabdulaaly, Lama, Baron, Roland, Rosen, Clifford J. 2020. The role of Zfp467 in mediating the pro-osteogenic and anti-adipogenic effects on bone and bone marrow niche. In Bone, 144, 115832. doi:10.1016/j.bone.2020.115832. https://pubmed.ncbi.nlm.nih.gov/33359894/
3. Quach, Julie M, Walker, Emma C, Allan, Elizabeth, Gillespie, Matthew T, Martin, T John. 2010. Zinc finger protein 467 is a novel regulator of osteoblast and adipocyte commitment. In The Journal of biological chemistry, 286, 4186-98. doi:10.1074/jbc.M110.178251. https://pubmed.ncbi.nlm.nih.gov/21123171/
4. You, Li, Chen, Lin, Pan, Ling, Gu, Wen-Sha, Chen, Jin-Yu. 2014. Zinc finger protein 467 regulates Wnt signaling by modulating the expression of sclerostin in adipose derived stem cells. In Biochemical and biophysical research communications, 456, 598-604. doi:10.1016/j.bbrc.2014.11.120. https://pubmed.ncbi.nlm.nih.gov/25490389/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen