C57BL/6NCya-Pink1em1flox/Cya
Common Name:
Pink1-flox
Product ID:
S-CKO-14356
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Pink1-flox
Strain ID
CKOCMP-68943-Pink1-B6N-VA
Gene Name
Product ID
S-CKO-14356
Gene Alias
1190006F07Rik; BRPK; mFLJ00387
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Pink1em1flox/Cya mice (Catalog S-CKO-14356) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030536
NCBI RefSeq
NM_026880
Target Region
Exon 2~3
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
PINK1, also known as PTEN-induced kinase 1, is a serine/threonine-protein kinase in mitochondria. It is crucial for mitochondrial quality control, especially in the regulation of mitophagy, a process that selectively degrades defective mitochondria [1,2,3,4,5,8,9]. PINK1 mainly functions in the PINK1/Parkin pathway, which is essential for many aspects of mitochondrial physiology [1,2,3,4,5]. Mitochondrial health is vital for cell survival, particularly in energy-intensive neuronal cells, and PINK1's role in maintaining this health has broad biological importance [1].
Loss-of-function mutations in PINK1 cause parkinsonism in humans and mitochondrial dysfunction in model organisms [7]. In Drosophila melanogaster, genetic epistasis between PINK1 and Parkin was biochemically explained by the finding that PINK1 accumulation on mitochondria is both necessary and sufficient for Parkin recruitment to mitochondria, and disease-causing mutations in PINK1 disrupt Parkin-induced mitophagy [7]. In cultured human fibroblasts and induced pluripotent stem cell-derived neurons with homozygous PARK7 mutations, DJ-1 was found to be an essential downstream mediator in PINK1/parkin-dependent mitophagy, suggesting disruption of this pathway as a common pathogenic mechanism in autosomal recessive Parkinson's disease [6]. In mouse models, Pink1 -/- mice showed a significant decrease in bone mass and collagen deposition after ovariectomy, with inhibited osteoblast differentiation due to impaired mitochondrial homeostasis, indicating PINK1's role in bone disease [8].
In conclusion, PINK1 is a key regulator of mitochondrial quality control, mainly through its role in the PINK1/Parkin-mediated mitophagy pathway. Studies using gene knockout models, such as in Drosophila and mice, have revealed its importance in neurodegenerative diseases like Parkinson's disease and in bone diseases. Understanding PINK1's function provides insights into the mechanisms of these diseases and potential therapeutic targets.
References:
1. Li, Jie, Yang, Dongming, Li, Zhiping, Zhao, Deming, Yang, Lifeng. 2022. PINK1/Parkin-mediated mitophagy in neurodegenerative diseases. In Ageing research reviews, 84, 101817. doi:10.1016/j.arr.2022.101817. https://pubmed.ncbi.nlm.nih.gov/36503124/
2. Narendra, Derek P, Youle, Richard J. 2024. The role of PINK1-Parkin in mitochondrial quality control. In Nature cell biology, 26, 1639-1651. doi:10.1038/s41556-024-01513-9. https://pubmed.ncbi.nlm.nih.gov/39358449/
3. Pickrell, Alicia M, Youle, Richard J. . The roles of PINK1, parkin, and mitochondrial fidelity in Parkinson's disease. In Neuron, 85, 257-73. doi:10.1016/j.neuron.2014.12.007. https://pubmed.ncbi.nlm.nih.gov/25611507/
4. Eiyama, Akinori, Okamoto, Koji. 2015. PINK1/Parkin-mediated mitophagy in mammalian cells. In Current opinion in cell biology, 33, 95-101. doi:10.1016/j.ceb.2015.01.002. https://pubmed.ncbi.nlm.nih.gov/25697963/
5. Nguyen, Thanh N, Padman, Benjamin S, Lazarou, Michael. 2016. Deciphering the Molecular Signals of PINK1/Parkin Mitophagy. In Trends in cell biology, 26, 733-744. doi:10.1016/j.tcb.2016.05.008. https://pubmed.ncbi.nlm.nih.gov/27291334/
6. Imberechts, Dorien, Kinnart, Inge, Wauters, Fieke, Verfaillie, Catherine, Vandenberghe, Wim. . DJ-1 is an essential downstream mediator in PINK1/parkin-dependent mitophagy. In Brain : a journal of neurology, 145, 4368-4384. doi:10.1093/brain/awac313. https://pubmed.ncbi.nlm.nih.gov/36039535/
7. Narendra, Derek P, Jin, Seok Min, Tanaka, Atsushi, Cookson, Mark R, Youle, Richard J. 2010. PINK1 is selectively stabilized on impaired mitochondria to activate Parkin. In PLoS biology, 8, e1000298. doi:10.1371/journal.pbio.1000298. https://pubmed.ncbi.nlm.nih.gov/20126261/
8. Lee, So-Young, An, Hyun-Ju, Kim, Jin Man, Lim, Ha Jeong, Lee, Soonchul. 2021. PINK1 deficiency impairs osteoblast differentiation through aberrant mitochondrial homeostasis. In Stem cell research & therapy, 12, 589. doi:10.1186/s13287-021-02656-4. https://pubmed.ncbi.nlm.nih.gov/34823575/
9. Wang, Nan, Zhu, Peining, Huang, Renxuan, Zhao, Hongyang, Gao, Yufei. 2020. PINK1: The guard of mitochondria. In Life sciences, 259, 118247. doi:10.1016/j.lfs.2020.118247. https://pubmed.ncbi.nlm.nih.gov/32805222/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen