C57BL/6JCya-Cnot8em1flox/Cya
Common Name:
Cnot8-flox
Product ID:
S-CKO-14423
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cnot8-flox
Strain ID
CKOCMP-69125-Cnot8-B6J-VA
Gene Name
Product ID
S-CKO-14423
Gene Alias
1500015I04Rik; 1810022F04Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cnot8em1flox/Cya mice (Catalog S-CKO-14423) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020822
NCBI RefSeq
NM_026949
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Cnot8, also known as hPop2/Caf1b/Calif, is a subunit of the Ccr4-Not complex with deadenylase activity. This complex is a key regulator of gene expression in eukaryotic cells, and Cnot8's deadenylase function is involved in mRNA turnover, which impacts various biological processes such as cell proliferation, pluripotency transition, and DNA damage response [2,4,5,6,7].
Knockout (KO) of Cnot8 in mice leads to early embryonic lethality, but Cnot8 KO embryonic stem cells (ESCs) can be established. These KO ESCs highly express many genes during differentiation into the formative state, including naïve-like genes, indicating that Cnot8 is essential for eliminating naïve regulation networks and for the naïve-to-formative pluripotency transition [1]. In zebrafish, a mutation in cnot8 increases mRNA levels of a subset of developmental control genes, suggesting its role in vertebrate development [3]. In human cells, depletion of CNOT8 reduces cell viability after ionizing radiation treatment, showing its involvement in the DNA damage response [2].
In summary, Cnot8 is crucial for mRNA turnover and has significant functions in processes like pluripotency transition, development, and DNA damage response. Studies using KO mouse models and other genetic models have revealed its essential roles, which are relevant to understanding embryonic development and cellular responses to DNA damage [1,2,3].
References:
1. Quan, Yujun, Wang, Meijiao, Xu, Chengpeng, Lu, Falong, Li, Lei. . Cnot8 eliminates naïve regulation networks and is essential for naïve-to-formative pluripotency transition. In Nucleic acids research, 50, 4414-4435. doi:10.1093/nar/gkac236. https://pubmed.ncbi.nlm.nih.gov/35390160/
2. Eskandarian, Samira, Grand, Roger, Irani, Shiva, Saeedi, Mohsen, Mirfakhraie, Reza. . Importance of CNOT8 Deadenylase Subunit in DNA Damage Responses Following Ionizing Radiation (IR). In Reports of biochemistry & molecular biology, 9, 163-170. doi:10.29252/rbmb.9.2.163. https://pubmed.ncbi.nlm.nih.gov/33178865/
3. Koch, Peter, Löhr, Heiko B, Driever, Wolfgang. 2014. A mutation in cnot8, component of the Ccr4-not complex regulating transcript stability, affects expression levels of developmental regulators and reveals a role of Fgf3 in development of caudal hypothalamic dopaminergic neurons. In PloS one, 9, e113829. doi:10.1371/journal.pone.0113829. https://pubmed.ncbi.nlm.nih.gov/25478689/
4. Aslam, Akhmed, Mittal, Saloni, Koch, Frederic, Andrau, Jean-Christophe, Winkler, G Sebastiaan. 2009. The Ccr4-NOT deadenylase subunits CNOT7 and CNOT8 have overlapping roles and modulate cell proliferation. In Molecular biology of the cell, 20, 3840-50. doi:. https://pubmed.ncbi.nlm.nih.gov/19605561/
5. Winkler, G Sebastiaan. . The mammalian anti-proliferative BTG/Tob protein family. In Journal of cellular physiology, 222, 66-72. doi:10.1002/jcp.21919. https://pubmed.ncbi.nlm.nih.gov/19746446/
6. Doidge, Rachel, Mittal, Saloni, Aslam, Akhmed, Winkler, G Sebastiaan. 2012. The anti-proliferative activity of BTG/TOB proteins is mediated via the Caf1a (CNOT7) and Caf1b (CNOT8) deadenylase subunits of the Ccr4-not complex. In PloS one, 7, e51331. doi:10.1371/journal.pone.0051331. https://pubmed.ncbi.nlm.nih.gov/23236473/
7. Zukeran, Ari, Takahashi, Akinori, Takaoka, Shohei, Ikematsu, Shinya, Yamamoto, Tadashi. 2016. The CCR4-NOT deadenylase activity contributes to generation of induced pluripotent stem cells. In Biochemical and biophysical research communications, 474, 233-239. doi:10.1016/j.bbrc.2016.03.119. https://pubmed.ncbi.nlm.nih.gov/27037025/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen