C57BL/6JCya-Mcemp1em1flox/Cya
Common Name:
Mcemp1-flox
Product ID:
S-CKO-14450
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mcemp1-flox
Strain ID
CKOCMP-69189-Mcemp1-B6J-VA
Gene Name
Product ID
S-CKO-14450
Gene Alias
1810033B17Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mcemp1em1flox/Cya mice (Catalog S-CKO-14450) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000014118
NCBI RefSeq
NM_026985
Target Region
Exon 3~7
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
MCEMP1, also known as C19ORF59, is a mast cell-expressed membrane protein. In lung mast cells, it functions as an adaptor for KIT, promoting SCF-mediated mast cell proliferation through its cytoplasmic immunoreceptor tyrosine-based activation motif and enhancing KIT autophosphorylation and activation [1]. It may also be involved in immune-related signaling pathways, as indicated by its association with various diseases related to immune responses [2,7].
In mouse models, Mcemp1 -deficient mice exhibit reduced airway inflammation and lung impairment in chronic asthma models, demonstrating that MCEMP1 is crucial for SCF-induced peritoneal mast cell proliferation in vitro and lung mast cell expansion in vivo [1]. In other diseases, such as advanced gastric cancer, high MCEMP1 expression is related to poor survival and is correlated with immune infiltration, potentially affecting prognosis by regulating immune cell function [2]. In septic shock, MCEMP1 is one of six hub genes identified as potential diagnostic markers [3]. In lung adenocarcinoma, low MCEMP1 expression promotes tumor progression [4]. In coronary artery disease, MCEMP1 may play an important role as it is associated with immune cell infiltration, and knockdown of MCEMP1 in HUVECs affects cell proliferation, apoptosis, and inflammatory factor expression [5]. In idiopathic pulmonary fibrosis, MCEMP1 is highly expressed in classical monocytes and alveolar macrophages, regulated by TGFβ, and participates in cell chemotaxis, adhesion, and migration [6,8].
In conclusion, MCEMP1 is a significant gene involved in mast cell-related functions and immune-related signaling. Its role in various disease conditions, as revealed by studies including those using mouse models, highlights its potential as a biomarker and therapeutic target in diseases such as asthma, cancer, septic shock, coronary artery disease, and idiopathic pulmonary fibrosis.
References:
1. Choi, Youn Jung, Yoo, Ji-Seung, Jung, Kyle, Asosingh, Kewal, Jung, Jae U. 2023. Lung-specific MCEMP1 functions as an adaptor for KIT to promote SCF-mediated mast cell proliferation. In Nature communications, 14, 2045. doi:10.1038/s41467-023-37873-3. https://pubmed.ncbi.nlm.nih.gov/37041174/
2. Wang, Daijun, Gu, Yanmei, Huo, Chengdong, Teng, Muzhou, Li, Yumin. 2022. MCEMP1 is a potential therapeutic biomarker associated with immune infiltration in advanced gastric cancer microenvironment. In Gene, 840, 146760. doi:10.1016/j.gene.2022.146760. https://pubmed.ncbi.nlm.nih.gov/35905854/
3. Kong, Chang, Zhu, Yurun, Xie, Xiaofan, Wu, Jiayu, Qian, Meizi. 2023. Six potential biomarkers in septic shock: a deep bioinformatics and prospective observational study. In Frontiers in immunology, 14, 1184700. doi:10.3389/fimmu.2023.1184700. https://pubmed.ncbi.nlm.nih.gov/37359526/
4. Ling, Liqun, Hu, Tianqi, Zhou, Chenkang, Wang, Yumin, Wang, Junjun. . Low Expression MCEMP1 Promotes Lung Adenocarcinoma Progression and its Clinical Value. In Current cancer drug targets, 25, 281-293. doi:10.2174/0115680096292054240409070618. https://pubmed.ncbi.nlm.nih.gov/38676531/
5. Ye, Wei, Shen, Bo, Tang, Qizhu, Xie, Lili, He, Qi. 2024. Identification of a novel immune infiltration-related gene signature, MCEMP1, for coronary artery disease. In PeerJ, 12, e18135. doi:10.7717/peerj.18135. https://pubmed.ncbi.nlm.nih.gov/39346078/
6. Perrot, Carole Y, Karampitsakos, Theodoros, Unterman, Avraham, Bandyopadhyay, Debabrata, Herazo-Maya, Jose D. 2023. Mast-Cell Expressed Membrane Protein-1 (MCEMP1) is expressed in classical monocytes and alveolar macrophages in Idiopathic Pulmonary Fibrosis and regulates cell chemotaxis, adhesion, and migration in a TGFβ dependent manner. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.10.07.561349. https://pubmed.ncbi.nlm.nih.gov/37873485/
7. Huang, Pu, Liu, Yawen, Jia, Baoqing. 2022. The Expression, Prognostic Value, and Immunological Correlation of MCEMP1 and its Potential Role in Gastric Cancer. In Journal of oncology, 2022, 8167496. doi:10.1155/2022/8167496. https://pubmed.ncbi.nlm.nih.gov/35378772/
8. Perrot, Carole Y, Karampitsakos, Theodoros, Unterman, Avraham, Bandyopadhyay, Debabrata, Herazo-Maya, Jose D. 2024. Mast-cell expressed membrane protein-1 is expressed in classical monocytes and alveolar macrophages in idiopathic pulmonary fibrosis and regulates cell chemotaxis, adhesion, and migration in a TGFβ-dependent manner. In American journal of physiology. Cell physiology, 326, C964-C977. doi:10.1152/ajpcell.00563.2023. https://pubmed.ncbi.nlm.nih.gov/38189137/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen