C57BL/6JCya-Gins1em1flox/Cya
Common Name:
Gins1-flox
Product ID:
S-CKO-14473
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gins1-flox
Strain ID
CKOCMP-69270-Gins1-B6J-VA
Gene Name
Product ID
S-CKO-14473
Gene Alias
2810418N01Rik; Gins4; PSF1; mKIAA0186
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gins1em1flox/Cya mice (Catalog S-CKO-14473) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028948
NCBI RefSeq
NM_027014
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
GINS1, a subunit of the GINS complex, functions in eukaryotic DNA replication, collaborating with the MCM2-7 complex and Cdc45. It plays a significant role in cell cycle modulation, which is crucial for DNA replication and cell proliferation [3,4].
GINS1 is overexpressed in multiple cancers such as leukemia, DLBCL, hepatocellular carcinoma, glioma, bladder cancer, gastric cancer, sarcoma, and lung adenocarcinoma, and is associated with poor prognosis in these malignancies. In DLBCL, the FOXP1-GINS1 axis promotes cell proliferation and doxorubicin resistance [1]. In hepatocellular carcinoma, GINS1 promotes ZEB1-mediated epithelial-mesenchymal transition and tumor metastasis via β-catenin signaling [2]. In glioma, GINS1 promotes cell proliferation and migration through USP15-mediated deubiquitination of TOP2A [3]. In bladder cancer, GINS1 promotes the initiation and progression by activating the AKT/mTOR/c-Myc signaling pathway [4]. In sarcoma, it is overexpressed in metastatic samples, and its alteration is linked to worse survival [5]. In lung adenocarcinoma, GINS1 facilitates development via Wnt/β-catenin activation [6].
In conclusion, GINS1 is essential in DNA replication and cell cycle-related processes. Its overexpression in various cancers reveals its significant role in cancer development and progression, making it a potential therapeutic target. Mouse models, if studied, could further elucidate its role in these disease conditions, providing more insights into its functions and potential treatments.
References：
1. Chen, Zhenfa, Wang, Ting, Li, Cui, Wang, Jundong, Li, Shufeng. 2023. FOXP1-GINS1 axis promotes DLBCL proliferation and directs doxorubicin resistance. In Journal of Cancer, 14, 2289-2300. doi:10.7150/jca.85906. https://pubmed.ncbi.nlm.nih.gov/37576391/
2. Liang, Junjie, Yao, Nan, Deng, Bo, Cao, Mingrong, Hong, Jian. 2024. GINS1 promotes ZEB1-mediated epithelial-mesenchymal transition and tumor metastasis via β-catenin signaling in hepatocellular carcinoma. In Journal of cellular physiology, 239, e31237. doi:10.1002/jcp.31237. https://pubmed.ncbi.nlm.nih.gov/38468464/
3. Yang, Hui, Liu, Xiaocen, Zhu, Xiaolong, Ma, Mingzhe, Lv, Kun. 2022. GINS1 promotes the proliferation and migration of glioma cells through USP15-mediated deubiquitination of TOP2A. In iScience, 25, 104952. doi:10.1016/j.isci.2022.104952. https://pubmed.ncbi.nlm.nih.gov/36065190/
4. Fu, Qiqi, Zheng, Hang, Wang, Xia, Liu, Tao, Peng, Jianping. 2024. GINS1 promotes the initiation and progression of bladder cancer by activating the AKT/mTOR/c-Myc signaling pathway. In Experimental cell research, 440, 114125. doi:10.1016/j.yexcr.2024.114125. https://pubmed.ncbi.nlm.nih.gov/38880324/
5. Zhao, Huanhuan, He, Xiaoxia, Ma, Zhanbei. . Identification of GINS1 as a potential prognostic biomarker for sarcoma using bioinformatic analysis. In Translational cancer research, 12, 1021-1032. doi:10.21037/tcr-23-524. https://pubmed.ncbi.nlm.nih.gov/37180662/
6. Ma, Luyuan, Li, Rongyang, Li, Pengyong, Si, Libo, Tian, Hui. 2025. GINS1 facilitates the development of lung adenocarcinoma via Wnt/β-catenin activation. In World journal of surgical oncology, 23, 122. doi:10.1186/s12957-025-03786-2. https://pubmed.ncbi.nlm.nih.gov/40197379/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen