C57BL/6JCya-Bod1em1flox/Cya
Common Name:
Bod1-flox
Product ID:
S-CKO-14568
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Bod1-flox
Strain ID
CKOCMP-69556-Bod1-B6J-VA
Gene Name
Product ID
S-CKO-14568
Gene Alias
2310022M17Rik; BOD2.1; Bodq1; Fam44b
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bod1em1flox/Cya mice (Catalog S-CKO-14568) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000058060
NCBI RefSeq
NM_001024919
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
BOD1, or Biorientation Defective 1, is a protein-coding gene. It is crucial for proper chromosome segregation during cell division, regulating phosphorylation of PLK1 substrates by modulating Protein Phosphatase 2A (PP2A) activity in mitosis [2,3,4]. BOD1 is also involved in the regulation of cell survival, fatty acid metabolism, and has been implicated in neural-related functions [5]. Genetic models, like Drosophila and mouse models, have been used to study its functions [2].
In Drosophila, neuron-specific knockdown of BOD1 led to learning deficits and synapse morphology abnormalities, suggesting its role in cognitive function [2]. In mice, BOD1 deficit in Purkinje cells of the cerebellar IV/V lobe attenuated cell excitability and spine density, causing ataxia behaviors. Conversely, BOD1 enrichment in these cells reversed hyperexcitability of CaMKIIα+ neurons in the fastigial nucleus and improved ataxia in L7-Cre; BOD1f/f mice, indicating its role in the cerebellar IV/V lobe-fastigial nucleus circuit related to motor coordination [1].
In conclusion, BOD1 is essential for chromosome segregation, cell survival, and fatty acid metabolism. Through gene-knockout or conditional-knockout mouse models, its role in motor coordination and cognitive function has been revealed. These findings suggest BOD1 could be a potential target for treating ataxia and intellectual disability [1,2,5].
References:
1. Liu, Xiu-Xiu, Chen, Xing-Hui, Zheng, Zhi-Wei, Han, Feng, Lu, Ying-Mei. 2022. BOD1 regulates the cerebellar IV/V lobe-fastigial nucleus circuit associated with motor coordination. In Signal transduction and targeted therapy, 7, 170. doi:10.1038/s41392-022-00989-x. https://pubmed.ncbi.nlm.nih.gov/35641478/
2. Esmaeeli-Nieh, Sahar, Fenckova, Michaela, Porter, Iain M, Schenck, Annette, Kuss, Andreas W. 2016. BOD1 Is Required for Cognitive Function in Humans and Drosophila. In PLoS genetics, 12, e1006022. doi:10.1371/journal.pgen.1006022. https://pubmed.ncbi.nlm.nih.gov/27166630/
3. Porter, Iain M, McClelland, Sarah E, Khoudoli, Guennadi A, Blow, J Julian, Swedlow, Jason R. 2007. Bod1, a novel kinetochore protein required for chromosome biorientation. In The Journal of cell biology, 179, 187-97. doi:. https://pubmed.ncbi.nlm.nih.gov/17938248/
4. Porter, Iain M, Schleicher, Katharina, Porter, Michael, Swedlow, Jason R. . Bod1 regulates protein phosphatase 2A at mitotic kinetochores. In Nature communications, 4, 2677. doi:10.1038/ncomms3677. https://pubmed.ncbi.nlm.nih.gov/24157919/
5. Hamdan, Nadine, Mehawej, Cybel, Sebaaly, Ghada, De Backer, O, Chouery, Eliane. . A homozygous stop gain mutation in BOD1 gene in a Lebanese patient with syndromic intellectual disability. In Clinical genetics, 98, 288-292. doi:10.1111/cge.13799. https://pubmed.ncbi.nlm.nih.gov/32578875/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen