C57BL/6JCya-Gpx8em1flox/Cya
Common Name:
Gpx8-flox
Product ID:
S-CKO-14581
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gpx8-flox
Strain ID
CKOCMP-69590-Gpx8-B6J-VA
Gene Name
Product ID
S-CKO-14581
Gene Alias
2310016C16Rik; GPx-8; GSHPx-8
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpx8em1flox/Cya mice (Catalog S-CKO-14581) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022282
NCBI RefSeq
NM_027127
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
GPX8, a member of the Glutathione Peroxidases (GPXs) family, is located in the endoplasmic reticulum lumen. It is involved in redox homoeostasis, and its active site contains cysteine residues instead of selenocysteine [5,10]. It may also be involved in protein folding and regulation of Ca2+ in the endoplasmic reticulum [10].
In gliomas, GPX8 acts as a tumor accelerator, facilitating cell contact between tumor cells and macrophages and promoting microglial migration [1]. In clear cell renal cell carcinoma, GPX8 promotes lipogenesis through the NNMT, regulating tumorigenesis [2]. In hepatocellular carcinoma, downregulation of GPX8 enhances the stemness and migration ability of HCC cells [3]. In oral cancer cells, GPX8 deficiency-induced oxidative stress reprograms the m6A epitranscriptome [4]. In esophageal squamous cell carcinoma, GPX8 regulates apoptosis and autophagy through the IRE1/JNK pathway [6]. In primary glioma and glioblastoma, GPX8 is associated with poor prognosis and may be a therapeutic target [7,8]. In non-small cell lung cancer, GPX8 promotes migration and invasion by regulating epithelial characteristics [9].
In conclusion, GPX8 plays diverse roles in various biological processes and disease conditions, especially in multiple types of cancers. Functional studies, including gene knockout-related models, have revealed its significance in tumorigenesis, metastasis, and redox-related regulation, providing potential therapeutic targets for cancer treatment.
References:
1. Chen, Zigui, Zheng, Dandan, Lin, Ziren, Xia, Ying, Luo, Qisheng. 2023. GPX8 regulates pan-apoptosis in gliomas to promote microglial migration and mediate immunotherapy responses. In Frontiers in immunology, 14, 1260169. doi:10.3389/fimmu.2023.1260169. https://pubmed.ncbi.nlm.nih.gov/37795080/
2. Nguyen, Tin Tin Manh, Nguyen, Thi Ha, Kim, Han Sun, Kim, Jin-Mo, Park, Sunghyouk. 2023. GPX8 regulates clear cell renal cell carcinoma tumorigenesis through promoting lipogenesis by NNMT. In Journal of experimental & clinical cancer research : CR, 42, 42. doi:10.1186/s13046-023-02607-2. https://pubmed.ncbi.nlm.nih.gov/36750850/
3. Tao, Chen-Yang, Wu, Xiao-Ling, Song, Shu-Shu, Shi, Ying-Hong, Fan, Jia. 2024. Downregulation of GPX8 in hepatocellular carcinoma: impact on tumor stemness and migration. In Cellular oncology (Dordrecht, Netherlands), 47, 1391-1403. doi:10.1007/s13402-024-00934-w. https://pubmed.ncbi.nlm.nih.gov/38607517/
4. Chen, Xun, Yuan, Lingyu, Zhang, Lejia, Zhao, Wei, Yu, Dongsheng. . GPX8 deficiency-induced oxidative stress reprogrammed m6A epitranscriptome of oral cancer cells. In Epigenetics, 18, 2208707. doi:10.1080/15592294.2023.2208707. https://pubmed.ncbi.nlm.nih.gov/37170591/
5. Brigelius-Flohé, Regina, Maiorino, Matilde. 2012. Glutathione peroxidases. In Biochimica et biophysica acta, 1830, 3289-303. doi:10.1016/j.bbagen.2012.11.020. https://pubmed.ncbi.nlm.nih.gov/23201771/
6. Yin, Xiang, Zhang, Peng, Xia, Ning, Weng, Li, Shang, Mingyi. 2022. GPx8 regulates apoptosis and autophagy in esophageal squamous cell carcinoma through the IRE1/JNK pathway. In Cellular signalling, 93, 110307. doi:10.1016/j.cellsig.2022.110307. https://pubmed.ncbi.nlm.nih.gov/35288240/
7. Yang, Zhao-Shou, Yang, Qin, Sun, Xiao-Xiao, Yang, Xiao-Rong, Jiang, Wen-Gong. 2022. GPX8 as a Novel Prognostic Factor and Potential Therapeutic Target in Primary Glioma. In Journal of immunology research, 2022, 8025055. doi:10.1155/2022/8025055. https://pubmed.ncbi.nlm.nih.gov/36052280/
8. Li, Sibo, Jiang, Xudong, Guan, Meicun, Cao, Yanfei, Zhang, Lina. 2022. The overexpression of GPX8 is correlated with poor prognosis in GBM patients. In Frontiers in genetics, 13, 898204. doi:10.3389/fgene.2022.898204. https://pubmed.ncbi.nlm.nih.gov/36061208/
9. Zhang, Jun, Liu, Yun, Guo, Yan, Zhao, Qiang. 2020. GPX8 promotes migration and invasion by regulating epithelial characteristics in non-small cell lung cancer. In Thoracic cancer, 11, 3299-3308. doi:10.1111/1759-7714.13671. https://pubmed.ncbi.nlm.nih.gov/32975378/
10. Pei, Jun, Pan, Xingyu, Wei, Guanghui, Hua, Yi. 2023. Research progress of glutathione peroxidase family (GPX) in redoxidation. In Frontiers in pharmacology, 14, 1147414. doi:10.3389/fphar.2023.1147414. https://pubmed.ncbi.nlm.nih.gov/36937839/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen