C57BL/6JCya-Fbxo7em1flox/Cya
Common Name
Fbxo7-flox
Product ID
S-CKO-14644
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-69754-Fbxo7-B6J-VA
Status
When using this mouse strain in a publication, please cite “Fbxo7-flox Mouse (Catalog S-CKO-14644) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Fbxo7-flox
Strain ID
CKOCMP-69754-Fbxo7-B6J-VA
Gene Name
Product ID
S-CKO-14644
Gene Alias
2410015K21Rik, A230052G17Rik
Background
C57BL/6JCya
Gene Full Name
F-box protein 7
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 10 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000130320
NCBI Transcript ID
NM_153195
Target Region
Exon 3~4
Size of Effective Region
~2.9 kb
Overview of Gene Research
Fbxo7, an F-box protein, is a substrate-recognition component of the SCF complex. It functions as an E3 ubiquitin ligase, recognizing and mediating ubiquitination of substrates, and is also involved in regulating the proteasome complex, mitophagy, cell cycle, cell proliferation, and germ cell differentiation [3]. Mutations in FBXO7 have been associated with Parkinson's disease-15 (PARKIN15), highlighting its importance in neurodegenerative disease research [3,4,5,6,8].
In hepatocellular carcinoma, FBXO7 inhibits serine synthesis by ubiquitinating and degrading PRMT1, which in turn impairs PHGDH methylation and activation, leading to reduced serine synthesis, increased ROS, and inhibited cell growth. Its down-regulation in human HCC tissues is inversely related to PRMT1 and PHGDH methylation levels [1].
In glioblastoma, FBXO7 confers mesenchymal properties and chemoresistance by controlling Rbfox2-mediated alternative splicing. It ubiquitinates Rbfox2, leading to its stabilization and regulating the splicing of mesenchymal genes [2].
In endometrial carcinoma, FBXO7 acts as a tumor suppressor by inhibiting INF2-associated mitochondrial division through ubiquitination and degradation of INF2 [7].
In conclusion, Fbxo7 plays crucial roles in multiple biological processes and diseases. In cancer, it can act as a regulator of tumor-related metabolic pathways, cell phenotypes, and mitochondrial function. In Parkinson's disease, its mutations are associated with the pathogenesis. Functional studies, especially those using genetic models, have been essential in uncovering these roles, providing potential targets for cancer therapy and understanding the pathophysiology of Parkinson's disease.
References：
1. Luo, Li, Wu, Xingyun, Fan, Jiawu, Jiang, Jingwen, Wang, Kui. 2024. FBXO7 ubiquitinates PRMT1 to suppress serine synthesis and tumor growth in hepatocellular carcinoma. In Nature communications, 15, 4790. doi:10.1038/s41467-024-49087-2. https://pubmed.ncbi.nlm.nih.gov/38839752/
2. Li, Shangbiao, Chen, Yanwen, Xie, Yuxin, Deng, Fan, Zhou, Aidong. 2023. FBXO7 Confers Mesenchymal Properties and Chemoresistance in Glioblastoma by Controlling Rbfox2-Mediated Alternative Splicing. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2303561. doi:10.1002/advs.202303561. https://pubmed.ncbi.nlm.nih.gov/37822160/
3. Zhong, Yeling, Li, Jinyun, Ye, Meng, Jin, Xiaofeng. 2022. The characteristics of FBXO7 and its role in human diseases. In Gene, 851, 146972. doi:10.1016/j.gene.2022.146972. https://pubmed.ncbi.nlm.nih.gov/36261086/
4. Conedera, Silvio, Apaydin, Hulya, Li, Yuanzhe, Nishioka, Kenya, Hattori, Nobutaka. 2016. FBXO7 mutations in Parkinson's disease and multiple system atrophy. In Neurobiology of aging, 40, 192.e1-192.e5. doi:10.1016/j.neurobiolaging.2016.01.003. https://pubmed.ncbi.nlm.nih.gov/26882974/
5. Jia, Fangzhi, Fellner, Avi, Kumar, Kishore Raj. 2022. Monogenic Parkinson's Disease: Genotype, Phenotype, Pathophysiology, and Genetic Testing. In Genes, 13, . doi:10.3390/genes13030471. https://pubmed.ncbi.nlm.nih.gov/35328025/
6. Randle, Suzanne J, Laman, Heike. . Structure and Function of Fbxo7/PARK15 in Parkinson's Disease. In Current protein & peptide science, 18, 715-724. doi:10.2174/1389203717666160311121433. https://pubmed.ncbi.nlm.nih.gov/26965690/
7. Zhang, Hui, Zhao, Yiting, Wang, Jie, Ye, Meng, Jin, Xiaofeng. 2023. FBXO7, a tumor suppressor in endometrial carcinoma, suppresses INF2-associated mitochondrial division. In Cell death & disease, 14, 368. doi:10.1038/s41419-023-05891-0. https://pubmed.ncbi.nlm.nih.gov/37344480/
8. Zhou, Zhi Dong, Lee, Ji Chao Tristan, Tan, Eng King. 2018. Pathophysiological mechanisms linking F-box only protein 7 (FBXO7) and Parkinson's disease (PD). In Mutation research. Reviews in mutation research, 778, 72-78. doi:10.1016/j.mrrev.2018.10.001. https://pubmed.ncbi.nlm.nih.gov/30454685/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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