C57BL/6JCya-Tnfaip8l2em1flox/Cya
Common Name
Tnfaip8l2-flox
Product ID
S-CKO-14647
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-69769-Tnfaip8l2-B6J-VA
When using this mouse strain in a publication, please cite “Tnfaip8l2-flox Mouse (Catalog S-CKO-14647) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Tnfaip8l2-flox
Strain ID
CKOCMP-69769-Tnfaip8l2-B6J-VA
Gene Name
Product ID
S-CKO-14647
Gene Alias
1810019A08Rik, TIPE2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 3
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000013851
NCBI RefSeq
NM_027206
Target Region
Exon 2
Size of Effective Region
~1.9 kb
Overview of Gene Research
Tnfaip8l2, also known as TIPE2, is a member of the tumor necrosis factor-α-induced protein 8 (TNFAIP8) family. It serves as a crucial negative regulator of both innate and adaptive immunity, playing a vital role in maintaining immune homeostasis by negatively regulating T cell receptor (TCR) and toll-like receptor (TLR) signal transduction [3]. It is also involved in the regulation of the RAC1-MTORC1 axis, which is related to autophagy, and the RHEB-MTORC1 pathway, which is associated with age-related hearing loss [1,2].
In Tnfaip8l2-deficient (Tnfaip8l2-/-) mouse models, features of oxidative stress in cochlear hair cells and age-related hearing degeneration were observed, similar to mTORC1-over-activated Tsc1-cKO mice. Rapamycin, an mTORC1 inhibitor, mitigated the hearing dysfunction caused by Tnfaip8l2-deficiency. Mechanistically, TNFAIP8L2 regulates mTORC1 signaling by inhibiting the GTPase activity of RHEB and RAC1 [2]. In an LPS-induced mouse endotoxemia model, TNFAIP8L2 deficiency exacerbated the inflammatory response and lung injury by controlling the MTOR activity [1].
In conclusion, Tnfaip8l2 is essential for maintaining immune homeostasis and regulating mTORC1-related biological processes. Gene-knockout mouse models have revealed its role in age-related hearing loss and endotoxemia-induced inflammatory responses, providing potential therapeutic targets for these diseases.
References：
1. Li, Wen, Li, Yulan, Guan, Yetong, Li, Yan, Zhang, Lining. 2020. TNFAIP8L2/TIPE2 impairs autolysosome reformation via modulating the RAC1-MTORC1 axis. In Autophagy, 17, 1410-1425. doi:10.1080/15548627.2020.1761748. https://pubmed.ncbi.nlm.nih.gov/32460619/
2. Li, Wen, Li, Yu, Wang, Min, Gao, Jiangang, Fu, Xiaolong. 2025. TNFAIP8L2 maintains hair cell function and regulates age-related hearing loss via mTORC1 signaling. In Molecular therapy : the journal of the American Society of Gene Therapy, , . doi:10.1016/j.ymthe.2025.03.046. https://pubmed.ncbi.nlm.nih.gov/40165373/
3. Gao, Jie, Zhang, Hanting, Zhang, Fang. 2023. Research progress of TIPE2 in immune-related diseases. In International immunopharmacology, 121, 110514. doi:10.1016/j.intimp.2023.110514. https://pubmed.ncbi.nlm.nih.gov/37348234/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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