C57BL/6JCya-Snhg8em1flox/Cya
Common Name:
Snhg8-flox
Product ID:
S-CKO-14682
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Contact for Pricing
Basic Information
Strain Name
Snhg8-flox
Strain ID
CKOCMP-69895-Snhg8-B6J-VA
Gene Name
Product ID
S-CKO-14682
Gene Alias
1110065M07Rik; 2010109N14Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Snhg8em1flox/Cya mice (Catalog S-CKO-14682) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000196466
NCBI RefSeq
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Target Region
Exon 1~3
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Snhg8, short for Small nucleolar RNA host gene 8, is a long non-coding RNA. It has physiological roles in epithelial and muscle satellite cells. Snhg8 can act as a molecular sponge for multiple miRNAs, regulating various target genes, and is involved in diverse biological processes and signaling pathways. Its dysregulation is associated with many human disorders, making it an important gene for understanding disease mechanisms [1].
In tauopathies, Snhg8 was significantly reduced in a mouse model and in affected human brains. Overexpression of mutant tau reduced Snhg8 expression and induced stress granule formation, while rescuing Snhg8 expression decreased stress granule formation, suggesting Snhg8 dysregulation drives stress granule formation via TIA1 in tauopathies [2].
In multiple cancer types like gastric, breast, and nasopharyngeal carcinomas, Snhg8 knockdown inhibited tumor cell proliferation, migration, and invasion. For example, in EBV-associated gastric cancer, Snhg8 promoted tumorigenesis and invasion by sponging miR-512-5p and upregulating TRIM28 [3]. In breast cancer, it functioned through the miR-634/ZBTB20 axis [4], and in nasopharyngeal carcinoma, via the miR-588/HMGA2 axis [5].
In osteogenic differentiation of periodontal ligament stem cells (PDLSCs), mechanical force decreased Snhg8 expression, which promoted osteogenic differentiation. Knockdown of Snhg8 in PDLSCs increased osteogenic-related gene expression [6].
In cerebral ischemia-reperfusion injury in mice, LncRNA Snhg8 improved the injury by inhibiting miR-494-3p expression, suppressing microglia inflammatory reactions and apoptosis [7].
In high-glucose-induced endothelial cell injury, silencing lncRNA Snhg8 reversed the decrease in eNOS activation, NO production, migration, and angiogenesis of primary human umbilical vein endothelial cells (pHUVECs) [8].
In diffuse large B-cell lymphoma, knockdown of Snhg8 inhibited cell proliferation and colony formation while promoting apoptosis by targeting miR-335-5p [9].
In nasopharyngeal carcinoma, Snhg8 promoted aggressive behaviors via regulating the miR-656-3p/SATB1 axis [10].
In conclusion, Snhg8 is a crucial long non-coding RNA involved in various biological processes and disease conditions. Studies using gene-knockout or knockdown models in different tissues and disease models have revealed its role in cancer progression, neurodegenerative diseases, osteogenic differentiation, and ischemia-reperfusion injury. These findings contribute to a better understanding of the molecular mechanisms underlying these diseases and may provide potential therapeutic targets.
References:
1. Ghafouri-Fard, Soudeh, Harsij, Atefeh, Hussen, Bashdar Mahmud, Taheri, Mohammad, Ayatollahi, Seyed Abdulmajid. 2023. A review on the role of SNHG8 in human disorders. In Pathology, research and practice, 245, 154458. doi:10.1016/j.prp.2023.154458. https://pubmed.ncbi.nlm.nih.gov/37043963/
2. Bhagat, Reshma, Minaya, Miguel A, Renganathan, Arun, Grinberg, Lea T, Karch, Celeste M. 2023. Long non-coding RNA SNHG8 drives stress granule formation in tauopathies. In Molecular psychiatry, 28, 4889-4901. doi:10.1038/s41380-023-02237-2. https://pubmed.ncbi.nlm.nih.gov/37730840/
3. Zou, Changyan, Liao, Jinrong, Hu, Dan, Huang, Tao, Lin, Xiandong. 2021. SNHG8 Promotes the Progression of Epstein-Barr Virus-Associated Gastric Cancer via Sponging miR-512-5p and Targeting TRIM28. In Frontiers in oncology, 11, 734694. doi:10.3389/fonc.2021.734694. https://pubmed.ncbi.nlm.nih.gov/34722282/
4. Xu, Xianyun, Xie, Qiongjun, Xie, Mingfeng, Zeng, Yong, Liu, Qian. 2021. LncRNA SNHG8 Serves as an Oncogene in Breast Cancer Through miR-634/ZBTB20 Axis. In Cancer management and research, 13, 3017-3028. doi:10.2147/CMAR.S270128. https://pubmed.ncbi.nlm.nih.gov/33854372/
5. Luan, Qiang, Yang, Ruifang, Lin, Lejun, Li, Xuecheng. 2022. SNHG8 promotes cell proliferation, migration, and invasion of nasopharyngeal carcinoma cells as an oncogene through miR-588/HMGA2 axis. In Canadian journal of physiology and pharmacology, 100, 158-166. doi:10.1139/cjpp-2021-0149. https://pubmed.ncbi.nlm.nih.gov/35080988/
6. Zhang, Zijie, He, Qin, Yang, Shuangyan, Li, Xiaoyu, Wei, Fulan. 2022. Mechanical force-sensitive lncRNA SNHG8 inhibits osteogenic differentiation by regulating EZH2 in hPDLSCs. In Cellular signalling, 93, 110285. doi:10.1016/j.cellsig.2022.110285. https://pubmed.ncbi.nlm.nih.gov/35192931/
7. Cao, T, Liu, Q, Pan, M, Zhang, X. . [LncRNA SNHG8 inhibits miR-494-3p expression to alleviate cerebral ischemia-reperfusion injury in mice]. In Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 43, 2015-2022. doi:10.12122/j.issn.1673-4254.2023.12.04. https://pubmed.ncbi.nlm.nih.gov/38189386/
8. Fan, Zongcheng, Chen, Xin, Wang, Laicheng, Lin, Yunchai, Peng, Feng. 2023. LncRNA SNHG8 regulates the migration and angiogenesis of pHUVECs induced by high glucose via the TRPM7/ERK1/2 signaling axis. In Scientific reports, 13, 22485. doi:10.1038/s41598-023-49779-7. https://pubmed.ncbi.nlm.nih.gov/38110485/
9. Yu, Bing, Wang, Bo, Wu, Zhuman, Gao, Xiaoyan, Zeng, Huilan. 2021. LncRNA SNHG8 Promotes Proliferation and Inhibits Apoptosis of Diffuse Large B-Cell Lymphoma via Sponging miR-335-5p. In Frontiers in oncology, 11, 650287. doi:10.3389/fonc.2021.650287. https://pubmed.ncbi.nlm.nih.gov/33816305/
10. Tian, Xiaoyan, Liu, Yuehui, Wang, Zhi, Wu, Shuhong. 2020. lncRNA SNHG8 promotes aggressive behaviors of nasopharyngeal carcinoma via regulating miR-656-3p/SATB1 axis. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 131, 110564. doi:10.1016/j.biopha.2020.110564. https://pubmed.ncbi.nlm.nih.gov/32920509/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen