C57BL/6JCya-Ica1lem1flox/Cya
Common Name:
Ica1l-flox
Product ID:
S-CKO-14801
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ica1l-flox
Strain ID
CKOCMP-70375-Ica1l-B6J-VA
Gene Name
Product ID
S-CKO-14801
Gene Alias
1700030B17Rik; Als2cr15; b2b3465Clo
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ica1lem1flox/Cya mice (Catalog S-CKO-14801) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027172
NCBI RefSeq
NM_027407
Target Region
Exon 3
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Ica1l, which has sequence similarities to ICA69 (also known as ICA1), is a gene with diverse functions. It forms BAR-domain complexes with PICK1, and this interaction is crucial for acrosome formation in spermiogenesis. Ica1l is expressed in testes and brain, and in testes, it is the major binding partner for PICK1 [3].
ICA1L has been associated with several diseases. In genetic studies, it was found that the expression of ICA1L is associated with lacunar stroke, a subtype of small-vessel stroke, through transcriptome-wide association study (TWAS) [1]. An integrative analysis also revealed that ICA1L was associated with small-vessel stroke (SVS) based on brain-derived data at both protein and transcriptional levels [2]. Additionally, a proteome-wide association study (PWAS) showed that ICA1L is significantly associated with SVS and non-lobar intracerebral haemorrhage (ICH), and the SMR results indicated a causal association of its protein expression levels in the brain with these traits [4]. In cellular myoid neoplasms, novel SRF-ICA1L fusions were identified in some spindle cell tumors, which may define a subset of neoplasms composed of immature smooth muscle cells and have potential for malignant behavior [5]. In the context of neurodegenerative and neurodevelopmental disorders, ICA1L was identified as a potential drug target in studies related to attention deficit hyperactivity disorder (ADHD) and Alzheimer's disease. In ADHD, it provided protection against both ASD and ADHD, while in Alzheimer's disease, its brain protein abundance was found to be causal, but it was not recommended as a prioritized drug due to potential side effects [6,7].
In conclusion, Ica1l is important for acrosome formation during spermiogenesis. In disease contexts, Ica1l has been linked to various cerebrovascular and neurological conditions, such as small-vessel stroke, intracerebral haemorrhage, ADHD, and Alzheimer's disease, highlighting its potential as a biomarker or drug target in these areas. The study of Ica1l using genetic models, such as the ICA1L-knockout mouse model which demonstrated its role in sperm development, helps in understanding its function in both normal biological processes and disease conditions [3].
References：
1. Traylor, Matthew, Persyn, Elodie, Tomppo, Liisa, Lewis, Cathryn M, Markus, Hugh S. 2021. Genetic basis of lacunar stroke: a pooled analysis of individual patient data and genome-wide association studies. In The Lancet. Neurology, 20, 351-361. doi:10.1016/S1474-4422(21)00031-4. https://pubmed.ncbi.nlm.nih.gov/33773637/
2. Wu, Bang-Sheng, Chen, Shu-Fen, Huang, Shu-Yi, Dong, Qiang, Yu, Jin-Tai. 2022. Identifying causal genes for stroke via integrating the proteome and transcriptome from brain and blood. In Journal of translational medicine, 20, 181. doi:10.1186/s12967-022-03377-9. https://pubmed.ncbi.nlm.nih.gov/35449099/
3. He, Jing, Xia, Mengying, Tsang, Wai Hung, Chow, King Lau, Xia, Jun. 2015. ICA1L forms BAR-domain complexes with PICK1 and is crucial for acrosome formation in spermiogenesis. In Journal of cell science, 128, 3822-36. doi:10.1242/jcs.173534. https://pubmed.ncbi.nlm.nih.gov/26306493/
4. Cullell, Natalia, Gallego-Fábrega, Cristina, Cárcel-Márquez, Jara, Fernández-Cadenas, Israel, Krupinski, Jerzy. 2022. ICA1L Is Associated with Small Vessel Disease: A Proteome-Wide Association Study in Small Vessel Stroke and Intracerebral Haemorrhage. In International journal of molecular sciences, 23, . doi:10.3390/ijms23063161. https://pubmed.ncbi.nlm.nih.gov/35328582/
5. Suurmeijer, Albert J, Dickson, Brendan C, Swanson, David, Zhang, Lei, Antonescu, Cristina R. . Novel SRF-ICA1L Fusions in Cellular Myoid Neoplasms With Potential For Malignant Behavior. In The American journal of surgical pathology, 44, 55-60. doi:10.1097/PAS.0000000000001336. https://pubmed.ncbi.nlm.nih.gov/31478943/
6. Zhang, Chengcheng, Jian, Lingqi, Li, Xiaojing, Hu, Xun, Li, Tao. 2024. Mendelian randomization analysis of the brain, cerebrospinal fluid, and plasma proteome identifies potential drug targets for attention deficit hyperactivity disorder. In EBioMedicine, 105, 105197. doi:10.1016/j.ebiom.2024.105197. https://pubmed.ncbi.nlm.nih.gov/38876042/
7. Ge, Yi-Jun, Ou, Ya-Nan, Deng, Yue-Ting, Tan, Lan, Yu, Jin-Tai. 2022. Prioritization of Drug Targets for Neurodegenerative Diseases by Integrating Genetic and Proteomic Data From Brain and Blood. In Biological psychiatry, 93, 770-779. doi:10.1016/j.biopsych.2022.11.002. https://pubmed.ncbi.nlm.nih.gov/36759259/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen