C57BL/6NCya-Cd248em1flox/Cya
Common Name:
Cd248-flox
Product ID:
S-CKO-14828
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cd248-flox
Strain ID
CKOCMP-70445-Cd248-B6N-VA
Gene Name
Product ID
S-CKO-14828
Gene Alias
2610111G01Rik; Cd164l1; Tem1
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Cd248em1flox/Cya mice (Catalog S-CKO-14828) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000070630
NCBI RefSeq
NM_054042
Target Region
Exon 1
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Cd248, also known as endosialin or tumor endothelial marker-1 (TEM-1), is a type 1 transmembrane glycoprotein expressed on activated mesenchymal cells. It functions during embryo development and is highly expressed in tumors and some fibrotic diseases, while being at low levels in adult tissues. Cd248 is involved in multiple pathways, such as the Wnt/β -catenin signaling pathway, and plays a significant role in angiogenesis, tumor growth, and fibrosis [2,5]. Gene knockout models are valuable for studying its functions.
In orthotopic lung cancer transplantation models, Cd248 -deficient (Cd248LacZ/LacZ) mice showed reduced tumor volume, vessel and pericyte density, and tumor vessel functionality compared to wild-type or haploinsufficient mice. Cd248 in pericytes derepresses Wnt signaling, upregulates angiogenic factors OPN and SERPINE1, and promotes angiogenesis and lung cancer growth. Administration of a β -catenin inhibitor in Cd248+/LacZ mice mimicked the effect of Cd248 loss, blocking the growth of transplanted lung tumor cells [1]. In a fibroblast-specific Cd248 gene knockout mouse model for non-small cell lung cancer (NSCLC), Cd248 knockout mice had a reduced ability to develop tumor lung metastasis. CD248+ cancer-associated fibroblasts (CAFs) activate the Hippo pathway, induce CTGF expression, facilitate the collagen I milieu of the stromal matrix, and promote NSCLC metastasis [4]. In diabetic kidney disease, Cd248 knockout ameliorated DKD-associated glomerular dysfunction and reversed maladaptive unfolded protein response signaling [3].
In conclusion, Cd248 is crucial in various biological processes and disease conditions. Through gene knockout mouse models, its role in promoting tumor growth and metastasis in lung cancer, and its contribution to diabetic kidney disease and pathologic scarring have been revealed. These findings suggest that Cd248 could be a potential therapeutic target for these diseases.
References:
1. Hong, Chia-Lun, Yu, I-Shing, Pai, Chen-Hsueh, Lin, Shu-Wha, Huang, Hsiang-Po. . CD248 Regulates Wnt Signaling in Pericytes to Promote Angiogenesis and Tumor Growth in Lung Cancer. In Cancer research, 82, 3734-3750. doi:10.1158/0008-5472.CAN-22-1695. https://pubmed.ncbi.nlm.nih.gov/35950912/
2. Teicher, Beverly A. 2019. CD248: A therapeutic target in cancer and fibrotic diseases. In Oncotarget, 10, 993-1009. doi:10.18632/oncotarget.26590. https://pubmed.ncbi.nlm.nih.gov/30847027/
3. Krishnan, Shruthi, Manoharan, Jayakumar, Wang, Hongjie, Isermann, Berend, Biemann, Ronald. 2022. CD248 induces a maladaptive unfolded protein response in diabetic kidney disease. In Kidney international, 103, 304-319. doi:10.1016/j.kint.2022.09.024. https://pubmed.ncbi.nlm.nih.gov/36309126/
4. Wu, Jiangwei, Zhang, Qiaoling, Yang, Zeyang, Zeng, Zhu, Wu, Jieheng. . CD248-expressing cancer-associated fibroblasts induce non-small cell lung cancer metastasis via Hippo pathway-mediated extracellular matrix stiffness. In Journal of cellular and molecular medicine, 28, e70025. doi:10.1111/jcmm.70025. https://pubmed.ncbi.nlm.nih.gov/39164826/
5. Valdez, Yanet, Maia, Margarida, Conway, Edward M. . CD248: reviewing its role in health and disease. In Current drug targets, 13, 432-9. doi:. https://pubmed.ncbi.nlm.nih.gov/22206249/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen