C57BL/6NCya-Prxl2aem1flox/Cya
Common Name:
Prxl2a-flox
Product ID:
S-CKO-14860
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Prxl2a-flox
Strain ID
CKOCMP-70564-Prxl2a-B6N-VA
Gene Name
Product ID
S-CKO-14860
Gene Alias
5730469M10Rik; Adrx; Fam213a; Pamm
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Prxl2aem1flox/Cya mice (Catalog S-CKO-14860) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022317
NCBI RefSeq
NM_027464
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Prxl2a, also known as peroxiredoxin like 2A, is an antioxidant protein. It protects cells from oxidative stress and may be involved in multiple biological processes related to redox regulation [1]. Oxidative stress is associated with various disease conditions, highlighting the importance of Prxl2a in maintaining cellular homeostasis.
In oral squamous cell carcinoma (OSCC), miR-125b downregulation leads to Prxl2a upregulation, protecting tumor cells from oxidative stress. Exogenous miR-125b expression in OSCC cells increases oxidative stress and drug sensitivity, similar to Prxl2a knockout, indicating an miR-125b-Prxl2a regulatory axis in OSCC pathogenesis [1].
In endometrial cancer, a prognostic signature including Prxl2a was established. Knockdown of Prxl2a significantly inhibited the proliferation and colony formation of Ishikawa and AN3CA cells, and suppressed xenograft growth [2].
In kidney renal clear cell carcinoma, Prxl2a was identified as one of six antioxidant genes significantly correlated with patient outcome, and high-risk patients (related to the six-gene signature including Prxl2a) had poorer prognosis [3].
In an Alzheimer's mouse model treated with cyanidin 3-O-glucoside, Prxl2a was among the upregulated antioxidant genes, suggesting its role in the antioxidant mechanism of the treatment [4].
In a mouse model of snakebite envenoming, Prxl2a was predicted as a biomarker sensitive to antivenom treatment, indicating its potential role in the pathophysiological changes after envenoming [5].
In conclusion, Prxl2a plays a crucial role in protecting cells from oxidative stress. Through gene-knockout or knockdown models in different disease conditions such as OSCC, endometrial cancer, kidney renal clear cell carcinoma, Alzheimer's disease, and snakebite envenoming, its functions in promoting tumor cell survival, affecting prognosis, and being involved in antioxidant and pathophysiological processes have been revealed. These findings contribute to a better understanding of the molecular mechanisms underlying these diseases and may provide potential therapeutic targets.
References：
1. Chen, Yi-Fen, Wei, Yun-Yen, Yang, Cheng-Chieh, Chang, Kuo-Wei, Lin, Shu-Chun. 2019. miR-125b suppresses oral oncogenicity by targeting the anti-oxidative gene PRXL2A. In Redox biology, 22, 101140. doi:10.1016/j.redox.2019.101140. https://pubmed.ncbi.nlm.nih.gov/30785086/
2. Yu, Jing, Yao, Hong-Wen, Liu, Ting-Ting, Ma, Sai, Wu, Ling-Ying. 2022. Comprehensive Analysis and Experimental Validation of a Novel Estrogen/Progesterone-Related Prognostic Signature for Endometrial Cancer. In Journal of personalized medicine, 12, . doi:10.3390/jpm12060914. https://pubmed.ncbi.nlm.nih.gov/35743699/
3. Ren, Xueting, Ma, Li, Wang, Nan, Kang, Huafeng, Zhou, Zhangjian. 2021. Antioxidant Gene Signature Impacts the Immune Infiltration and Predicts the Prognosis of Kidney Renal Clear Cell Carcinoma. In Frontiers in genetics, 12, 721252. doi:10.3389/fgene.2021.721252. https://pubmed.ncbi.nlm.nih.gov/34490047/
4. Jaiswal, Varun, Park, Miey, Lee, Hae-Jeung. 2021. Comparative Transcriptome Analysis of the Expression of Antioxidant and Immunity Genes in the Spleen of a Cyanidin 3-O-Glucoside-Treated Alzheimer's Mouse Model. In Antioxidants (Basel, Switzerland), 10, . doi:10.3390/antiox10091435. https://pubmed.ncbi.nlm.nih.gov/34573067/
5. Bala, Auwal A, Bedraoui, Anas, El Mejjad, Salim, Daouda, Tariq, Galan, Jacob A. 2025. Bioinformatics-Guided Identification and Quantification of Biomarkers of Crotalus atrox Envenoming and Its Neutralization by Antivenom. In Molecular & cellular proteomics : MCP, 24, 100956. doi:10.1016/j.mcpro.2025.100956. https://pubmed.ncbi.nlm.nih.gov/40147718/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen