C57BL/6JCya-Klhl30em1flox/Cya
Common Name:
Klhl30-flox
Product ID:
S-CKO-14914
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Klhl30-flox
Strain ID
CKOCMP-70788-Klhl30-B6J-VA
Gene Name
Product ID
S-CKO-14914
Gene Alias
4631423F02Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Klhl30em1flox/Cya mice (Catalog S-CKO-14914) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027533
NCBI RefSeq
NM_027551
Target Region
Exon 3
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
KLHL30, a Kelch-like gene, is emerging as an important regulator in various biological processes. It has been linked to pathways related to muscle development and circadian rhythm. In muscle, it may play a vital role in myogenesis, and in the context of circadian rhythm, it could potentially influence sleep-related phenotypes and cancer-related processes [1,2,4].
In myoblasts, KLHL30 has been shown to be a muscle-specific regulator. Its overexpression upregulates myogenic transcription factors (MYOD, MYOG, MEF2C), inducing myoblast differentiation and myotube formation, while knockdown has the opposite effect. It also significantly decreases the number of cells in the S stage, depressing myoblast proliferation [1]. In Nelore cattle, KLHL30 was identified as a hub gene in a co-expression network related to intramuscular fat content, though its role in lipid metabolism remains unclear [3]. In glioma, differential expression of KLHL30 was observed in glioblastoma multiforme versus normal brain, suggesting its potential involvement in glioma onset [2].
In conclusion, KLHL30 is essential for myoblast proliferation and differentiation, potentially impacts lipid metabolism in cattle, and may be associated with glioma. The study of KLHL30 through genetic models could further elucidate its functions in muscle development, lipid biology, and cancer-related processes, providing insights into related disease mechanisms and potential treatment strategies [1,2,3].
References：
1. Chen, Genghua, Yin, Yunqian, Lin, Zetong, Chen, Jiahui, Luo, Wen. 2021. Transcriptome profile analysis reveals KLHL30 as an essential regulator for myoblast differentiation. In Biochemical and biophysical research communications, 559, 84-91. doi:10.1016/j.bbrc.2021.04.086. https://pubmed.ncbi.nlm.nih.gov/33933993/
2. Madden, Melissa H, Anic, Gabriella M, Thompson, Reid C, Monteiro, Alvaro N, Egan, Kathleen M. 2013. Circadian pathway genes in relation to glioma risk and outcome. In Cancer causes & control : CCC, 25, 25-32. doi:10.1007/s10552-013-0305-y. https://pubmed.ncbi.nlm.nih.gov/24135790/
3. Dos Santos Silva, Danielly Beraldo, Fonseca, Larissa Fernanda Simielli, Pinheiro, Daniel Guariz, Chardulo, Luis Artur Loyola, de Albuquerque, Lucia Galvão. 2019. Prediction of hub genes associated with intramuscular fat content in Nelore cattle. In BMC genomics, 20, 520. doi:10.1186/s12864-019-5904-x. https://pubmed.ncbi.nlm.nih.gov/31238883/
4. LeVan, Tricia D, Xiao, Peng, Kumar, Gaurav, Cowan, Kenneth, Berger, Ann M. 2019. Genetic Variants in Circadian Rhythm Genes and Self-Reported Sleep Quality in Women with Breast Cancer. In Journal of circadian rhythms, 17, 6. doi:10.5334/jcr.184. https://pubmed.ncbi.nlm.nih.gov/31303884/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen