C57BL/6JCya-D7Ertd443eem1flox/Cya
Common Name:
D7Ertd443e-flox
Product ID:
S-CKO-14981
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
D7Ertd443e-flox
Strain ID
CKOCMP-71007-D7Ertd443e-B6J-VA
Gene Name
Product ID
S-CKO-14981
Gene Alias
4933400E14Rik; FATS; Gm497
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-D7Ertd443eem1flox/Cya mice (Catalog S-CKO-14981) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000172947
NCBI RefSeq
NM_001199941
Target Region
Exon 3
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
D7Ertd443e is an alias for FATS (fragile site-associated tumor suppressor). FATS, an (E2-independent) E3 ubiquitin-conjugating enzyme, is involved in tumor-related processes. It may be associated with pathways regulating the immune response in the context of cancer, with its function having potential implications for understanding tumor development and immunity [1].
Fats-/-mice (KO mouse model) show reduced subcutaneous B16 melanoma and H7 pancreatic tumor growth compared to WT controls. This reduced growth is macrophage-dependent and linked to a shift of macrophages within the tumor from tumor-promoting M2-like to antitumor M1-like macrophages. FATS deficiency promotes M1 polarization by disrupting NF-κB/IκBα negative feedback loops, stimulating and prolonging NF-κB activation. It also indirectly enhances CD4+ T helper type 1 (Th1) and cytotoxic T lymphocyte (CTL) adaptive immune responses to promote tumor regression [1].
In conclusion, FATS (D7Ertd443e) functions as an immune regulator in tumor development. The Fats-/-mouse model has revealed its role in promoting antitumor immunity, suggesting its potential as a target in cancer immunotherapy.
References:
1. Zhang, Lijuan, Zhang, Kai, Zhang, Jieyou, Yao, Zhi, Zhang, Rongxin. 2021. Loss of fragile site-associated tumor suppressor promotes antitumor immunity via macrophage polarization. In Nature communications, 12, 4300. doi:10.1038/s41467-021-24610-x. https://pubmed.ncbi.nlm.nih.gov/34262035/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen