C57BL/6JCya-Foxn3em1flox/Cya
Common Name:
Foxn3-flox
Product ID:
S-CKO-15062
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Foxn3-flox
Strain ID
CKOCMP-71375-Foxn3-B6J-VA
Gene Name
Product ID
S-CKO-15062
Gene Alias
5430426H20Rik; Ches1; Ches1l; HTLFL1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Foxn3em1flox/Cya mice (Catalog S-CKO-15062) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000085108
NCBI RefSeq
NM_183186
Target Region
Exon 4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
FOXN3, also known as CHES1, belongs to the forkhead box (FOX) protein family and acts as a transcriptional inhibitor. It is involved in cell cycle regulation, tumorigenesis, and metabolism [2,3]. It participates in the NF-κB, Smad, and AKT/MDM2/p53 signaling pathways, influencing processes like inflammation, fibrosis, and cell growth [1,5,6]. Genetic models such as transgenic and knockout zebrafish, and mouse models have been crucial for studying its functions [3,6].
In the context of lung inflammation, p38-mediated phosphorylation of FOXN3 at S83 and S85 residues promotes NF-κB activation, as phosphorylated FOXN3 dissociates from hnRNPU, leading to β-TrCP-mediated IκBα degradation. Genetic ablation of FOXN3 phosphorylation confers resistance to MRSA-induced pulmonary inflammatory injury [1]. In ovarian cancer, overexpression of FOXN3 inhibits cell proliferation, migration, invasion, and angiogenesis by negatively regulating RPS15A [4]. In glioma, FOXN3 inhibits cell growth and invasion through inactivating the AKT/MDM2/p53 signaling pathway [6]. In the liver, knockdown of Foxn3 expression decreases fasting glucose and modulates gluconeogenic substrate selection [3].
In summary, FOXN3 plays essential roles in inflammation, tumorigenesis, and glucose metabolism. Model-based research, especially gene knockout studies, has revealed its significant contributions to these biological processes and disease conditions, such as pulmonary inflammatory injury, ovarian cancer, glioma, and glucose regulation.
References:
1. Zhu, Xinxing, Huang, Beijia, Zhao, Fengting, Zhang, Chen, Lin, Juntang. . p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway. In Nucleic acids research, 51, 2195-2214. doi:10.1093/nar/gkad057. https://pubmed.ncbi.nlm.nih.gov/36794705/
2. Kong, Xiangyi, Zhai, Jie, Yan, Chengrui, Brown, James A L, Fang, Yi. 2019. Recent Advances in Understanding FOXN3 in Breast Cancer, and Other Malignancies. In Frontiers in oncology, 9, 234. doi:10.3389/fonc.2019.00234. https://pubmed.ncbi.nlm.nih.gov/31214487/
3. Karanth, Santhosh, Chaurasia, Bhagirath, Bowman, Faith M, Summers, Scott A, Schlegel, Amnon. . FOXN3 controls liver glucose metabolism by regulating gluconeogenic substrate selection. In Physiological reports, 7, e14238. doi:10.14814/phy2.14238. https://pubmed.ncbi.nlm.nih.gov/31552709/
4. Yang, Hua, Li, Mingyu, Qi, Yue. 2023. FOXN3 inhibits the progression of ovarian cancer through negatively regulating the expression of RPS15A. In Human cell, 36, 1120-1134. doi:10.1007/s13577-023-00876-9. https://pubmed.ncbi.nlm.nih.gov/37016167/
5. Yu, Jinjin, Li, Yingke, Li, Yiming, Li, Wei, Zhu, Xinxing. 2025. Phosphorylation of FOXN3 by NEK6 promotes pulmonary fibrosis through Smad signaling. In Nature communications, 16, 1865. doi:10.1038/s41467-025-56922-7. https://pubmed.ncbi.nlm.nih.gov/39984467/
6. Wang, Chaojia, Tu, Hanjun, Yang, Ling, Luo, Jie, Wang, Hui. 2021. FOXN3 inhibits cell proliferation and invasion via modulating the AKT/MDM2/p53 axis in human glioma. In Aging, 13, 21587-21598. doi:10.18632/aging.203499. https://pubmed.ncbi.nlm.nih.gov/34511432/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen