C57BL/6JCya-Sfpqem1flox/Cya
Common Name:
Sfpq-flox
Product ID:
S-CKO-15086
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Sfpq-flox
Strain ID
CKOCMP-71514-Sfpq-B6J-VA
Gene Name
Product ID
S-CKO-15086
Gene Alias
1110004P21Rik; 2810416M14Rik; 5730453G22Rik; 9030402K04Rik; D4Ertd314e; Gm12940; PSF; REP1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sfpqem1flox/Cya mice (Catalog S-CKO-15086) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030623
NCBI RefSeq
NM_023603
Target Region
Exon 2~4
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Sfpq, also known as splicing factor proline-and glutamine-rich, is a prion-like RNA-binding protein. It is involved in multiple cellular processes such as pre-mRNA splicing, transcriptional regulation, and DNA damage repair. Sfpq is a key component of paraspeckles in the cell nucleus, and its functions are diverse, with implications in various biological pathways and disease-related processes [3,6].
In cancer research, Sfpq has been shown to suppress TGF-β signaling by sequestering Smad4 in its condensates, dampening Smad-dependent transcriptional responses. Sfpq deficiency renders human cells hypersensitive to TGF-β responses, indicating its role in suppressing TGF-β tumor-suppressive activity [1]. In melanoma, knockdown of Sfpq expression decelerates several cancer-associated cell phenotypes, and Sfpq-RNA interactions promote the expression of oncogenic transcripts, suggesting it is an important driver of melanoma [4]. In non-small-cell lung cancer, Sfpq and its isoform show differential expression between cancer and control cells, and could potentially serve as diagnostic and prognostic markers [7].
In the context of viral infections, Sfpq represses Epstein-Barr virus (EBV) lytic reactivation by promoting histone H1 expression, highlighting its role in maintaining EBV latency [5].
In neurodegenerative diseases like Parkinson's disease and dementia with Lewy bodies, nuclear aggregates of NONO/Sfpq and A-to-I-edited RNA are found, which may offer new insights into the inclusion composition and pathophysiology of these diseases [2].
In conclusion, Sfpq is a multifunctional RNA-binding protein with significant roles in cancer, viral latency, and neurodegenerative diseases. Research on Sfpq, including through gene knockout or knockdown models, has provided valuable insights into its functions in these biological processes and disease conditions, contributing to a better understanding of the underlying molecular mechanisms and potentially guiding the development of new therapeutic strategies.
References:
1. Xiao, Mu, Wang, Fei, Chen, Nuo, Lin, Xia, Feng, Xin-Hua. 2023. Smad4 sequestered in SFPQ condensates prevents TGF-β tumor-suppressive signaling. In Developmental cell, 59, 48-63.e8. doi:10.1016/j.devcel.2023.11.020. https://pubmed.ncbi.nlm.nih.gov/38103553/
2. Belur, Nandkishore R, Bustos, Bernabe I, Lubbe, Steven J, Mazzulli, Joseph R. 2024. Nuclear aggregates of NONO/SFPQ and A-to-I-edited RNA in Parkinson's disease and dementia with Lewy bodies. In Neuron, 112, 2558-2580.e13. doi:10.1016/j.neuron.2024.05.003. https://pubmed.ncbi.nlm.nih.gov/38761794/
3. Shadrina, O A, Kikhay, T F, Agapkina, Yu Yu, Gottikh, M B. . [SFPQ and NONO Proteins and Long Non-Coding NEAT1 RNA: Cellular Functions and Role in the HIV-1 Life Cycle]. In Molekuliarnaia biologiia, 56, 259-274. doi:10.31857/S0026898422020161. https://pubmed.ncbi.nlm.nih.gov/35403619/
4. Bi, O, Anene, C A, Nsengimana, J, Newton-Bishop, J, Boyne, J R. 2021. SFPQ promotes an oncogenic transcriptomic state in melanoma. In Oncogene, 40, 5192-5203. doi:10.1038/s41388-021-01912-4. https://pubmed.ncbi.nlm.nih.gov/34218270/
5. Murray-Nerger, Laura A, Lozano, Clarisel, Burton, Eric M, Guo, Rui, Gewurz, Benjamin E. 2024. The nucleic acid binding protein SFPQ represses EBV lytic reactivation by promoting histone H1 expression. In Nature communications, 15, 4156. doi:10.1038/s41467-024-48333-x. https://pubmed.ncbi.nlm.nih.gov/38755141/
6. Lim, Yee Wa, James, Dylan, Huang, Jie, Lee, Mihwa. 2020. The Emerging Role of the RNA-Binding Protein SFPQ in Neuronal Function and Neurodegeneration. In International journal of molecular sciences, 21, . doi:10.3390/ijms21197151. https://pubmed.ncbi.nlm.nih.gov/32998269/
7. Yang, Libang, Gilbertsen, Adam, Jacobson, Blake, Henke, Craig A, Kratzke, Robert A. 2023. SFPQ and Its Isoform as Potential Biomarker for Non-Small-Cell Lung Cancer. In International journal of molecular sciences, 24, . doi:10.3390/ijms241512500. https://pubmed.ncbi.nlm.nih.gov/37569873/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen