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C57BL/6JCya-Fbxo9em1flox/Cya
Common Name:
Fbxo9-flox
Product ID:
S-CKO-15093
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fbxo9-flox
Strain ID
CKOCMP-71538-Fbxo9-B6J-VA
Gene Name
Fbxo9
Product ID
S-CKO-15093
Gene Alias
9030401P18Rik
Background
C57BL/6JCya
NCBI ID
71538
Modification
Conditional knockout
Chromosome
9
Phenotype
MGI:1918788
Document
Click here to download >>
Application
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More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fbxo9em1flox/Cya mice (Catalog S-CKO-15093) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001402
NCBI RefSeq
NM_001081490
Target Region
Exon 4~5
Size of Effective Region
~2.3 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Fbxo9, an F-box protein, serves as a substrate receptor for the SKP1-cullin-1-RBX1 ubiquitin ligase. It is involved in multiple biological processes by regulating the ubiquitination and degradation of target proteins, which impacts pathways like cell proliferation, apoptosis, and differentiation [4]. Genetic models, such as gene knockout mouse models, have been crucial in studying its function.

In acute myeloid leukemia (AML), deletion of Fbxo9 in a murine hematopoietic system using a Nuclease technology-based conditional knockout mouse model showed that loss of Fbxo9 led to accelerated and more aggressive leukemia development. Tumors lacking Fbxo9 highly expressed proteins related to metastasis and invasion, and had increased proteasome activity. Tumor cells were also more sensitive to in vitro proteasome inhibition with bortezomib, suggesting Fbxo9 expression may predict patient response to this drug [3].

In hepatocellular carcinoma (HCC), loss-and gain-of-function experiments demonstrated that FBXO9 was overexpressed and facilitated cell proliferation and metastasis both in vitro and in vivo. It is regulated by ZNF143 and targets FBXW7 for ubiquitination and degradation [2].

In lung cancer, knockdown of FBXO9 using lentiviral vectors carrying miRNA-based shRNA sequences promoted metastasis, while overexpression using doxycycline-inducible lentiviral constructs suppressed cell migration, tumor sphere growth, and metastasis by impeding V-ATPase assembly [1].

In summary, Fbxo9 plays diverse and significant roles in various biological processes and disease conditions. The use of knockout and conditional knockout mouse models has been instrumental in uncovering its functions in diseases like AML, HCC, and lung cancer, providing insights into potential therapeutic strategies targeting Fbxo9.

References:
1. Liu, Liang, Chen, Xiaodong, Wu, Leilei, Wei, Dongping, Xu, Yaping. 2024. Ubiquitin ligase subunit FBXO9 inhibits V-ATPase assembly and impedes lung cancer metastasis. In Experimental hematology & oncology, 13, 32. doi:10.1186/s40164-024-00497-4. https://pubmed.ncbi.nlm.nih.gov/38486234/
2. Wang, Zhenyu, Chen, Xiaoxia, Zhou, Lianer, Li, Hong, Li, Jinjun. 2022. FBXO9 Mediates the Cancer-Promoting Effects of ZNF143 by Degrading FBXW7 and Facilitates Drug Resistance in Hepatocellular Carcinoma. In Frontiers in oncology, 12, 930220. doi:10.3389/fonc.2022.930220. https://pubmed.ncbi.nlm.nih.gov/35847937/
3. Hynes-Smith, R Willow, Swenson, Samantha A, Vahle, Heather, Hyde, R Katherine, Buckley, Shannon M. 2019. Loss of FBXO9 Enhances Proteasome Activity and Promotes Aggressiveness in Acute Myeloid Leukemia. In Cancers, 11, . doi:10.3390/cancers11111717. https://pubmed.ncbi.nlm.nih.gov/31684170/
4. Hussain, Shujaat, Dong, Jianshu, Ma, Xinli, Clement, Agboybor, Liu, Hongmin. 2022. F-box only protein 9 and its role in cancer. In Molecular biology reports, 49, 1537-1544. doi:10.1007/s11033-021-07057-7. https://pubmed.ncbi.nlm.nih.gov/35025031/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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