C57BL/6NCya-Tmt1bem1flox/Cya
Common Name:
Tmt1b-flox
Product ID:
S-CKO-15118
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tmt1b-flox
Strain ID
CKOCMP-71664-Tmt1b-B6N-VA
Gene Name
Product ID
S-CKO-15118
Gene Alias
0610006F02Rik; Mettl7b
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Tmt1bem1flox/Cya mice (Catalog S-CKO-15118) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026398
NCBI RefSeq
NM_027853
Target Region
Exon 1
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Tmt1b, formerly known as METTL7B, is a methyltransferase. It has been associated with alkyl thiol methyltransferase activity in the liver, and might act on protein substrates as well as small molecule alkyl thiol substrates like hydrogen sulfide [2]. Tmt1b is localized to the stacked Golgi, endoplasmic reticulum, and lipid droplets, and is upregulated in various cancer types including lung cancer, gliomas, and leukemia, suggesting its potential oncogenic role [1].
Despite evidence of its activity on certain substrates, its normal physiological role remains poorly characterized. Animal models, like those used to study TMT1A's conserved ability to methylate thiol-based histone deacetylase inhibitors, may be useful in elucidating Tmt1b's role in humans, given that Tmt1b was not found in chicken or zebrafish [3,4]. Also, in cancer cells, Tmt1b (METTL7B) and CD274 (PD-L1) were found to be mutually exclusively expressed at either the mRNA or protein level, with METTL7B negatively regulating PD-L1 expression [5].
In summary, Tmt1b is a methyltransferase with potential roles in alkyl thiol methylation and cancer-related processes. Although its physiological function is not fully understood, research using animal models and studies on its expression in cancer cells are providing insights into its role in normal and disease conditions, especially in cancer.
References:
1. Denford, Sarah E, Wilhelm, Brian T. 2023. Defining the elusive oncogenic role of the methyltransferase TMT1B. In Frontiers in oncology, 13, 1211540. doi:10.3389/fonc.2023.1211540. https://pubmed.ncbi.nlm.nih.gov/37456227/
2. Russell, Drake A, Chau, Marvin K, Shi, Yuanyuan, Maldonato, Benjamin J, Totah, Rheem A. 2023. METTL7A (TMT1A) and METTL7B (TMT1B) Are Responsible for Alkyl S-Thiol Methyl Transferase Activity in Liver. In Drug metabolism and disposition: the biological fate of chemicals, 51, 1024-1034. doi:10.1124/dmd.123.001268. https://pubmed.ncbi.nlm.nih.gov/37137720/
3. González Dalmasy, José M, Fitzsimmons, Christina M, Frye, William J E, Robey, Robert W, Gottesman, Michael M. 2024. The thiol methyltransferase activity of TMT1A (METTL7A) is conserved across species. In Chemico-biological interactions, 394, 110989. doi:10.1016/j.cbi.2024.110989. https://pubmed.ncbi.nlm.nih.gov/38574836/
4. González Dalmasy, José M, Fitzsimmons, Christina M, Frye, William J E, Robey, Robert W, Gottesman, Michael M. 2023. The thiol methyltransferase activity of TMT1A (METTL7A) is conserved across species. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.11.17.567538. https://pubmed.ncbi.nlm.nih.gov/38076968/
5. Tang, Ling, Hu, Yuzhe, Wang, Chao, Han, Wenling, Wang, Pingzhang. 2025. Analysis of mutually exclusive expression in cancer cells identifies a previously unknown intergenic regulatory paradigm. In The FEBS journal, , . doi:10.1111/febs.70089. https://pubmed.ncbi.nlm.nih.gov/40186387/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen