C57BL/6JCya-Nanogem1flox/Cya
Common Name:
Nanog-flox
Product ID:
S-CKO-15276
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Nanog-flox
Strain ID
CKOCMP-71950-Nanog-B6J-VA
Gene Name
Product ID
S-CKO-15276
Gene Alias
2410002E02Rik; ENK; Stm1; ecat4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nanogem1flox/Cya mice (Catalog S-CKO-15276) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000012540
NCBI RefSeq
NM_028016
Target Region
Exon 2
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Nanog is a homeodomain transcription factor and a key stem cell transcription factor. It is crucial for maintaining the self-renewal and pluripotency in normal embryonic stem cells, inhibiting differentiation [4,5]. Nanog is involved in multiple signaling pathways, such as JAK/STAT and Wnt/β-catenin pathways [2]. It plays a vital role in embryonic development, especially during the preimplantation development phase, and its expression progressively decreases during embryonic stem cells differentiation, regulating embryonic and fetal development [1].
In cancerogenesis, Nanog re-expression is detected, starting from precancerous lesions. High Nanog expression in cancer is associated with advanced stage, poor differentiation, worse overall survival, and resistance to treatment. It enables cancer cells to obtain stem-cell like properties, which contribute to expanding growth, tumor maintenance, metastasis formation, and tumor relapse [1,2,3,4,5,6,7,8]. For example, in glioblastoma, forced expression of Nanog promotes the sphere formation and migration of GBM cells, enhancing the expression of SHH in the Hedgehog signaling pathway, and reduction of Nanog might have therapeutic benefits [6]. In ovarian cancer, Nanog is involved in tumorigenesis and chemoresistance, regulating the stemness of cancer stem cells [7].
In conclusion, Nanog is essential for normal development and its dysregulation is closely related to cancerogenesis. Research on Nanog, especially through functional studies in model organisms, helps to understand the mechanisms of embryonic development and cancer, providing potential therapeutic targets for cancer treatment.
References:
1. Grubelnik, Gašper, Boštjančič, Emanuela, Pavlič, Ana, Kos, Marina, Zidar, Nina. 2020. NANOG expression in human development and cancerogenesis. In Experimental biology and medicine (Maywood, N.J.), 245, 456-464. doi:10.1177/1535370220905560. https://pubmed.ncbi.nlm.nih.gov/32041418/
2. Vasefifar, Parisa, Motafakkerazad, Rouhollah, Maleki, Leili Aghebati, Baghbanzadeh, Amir, Baradaran, Behzad. 2022. Nanog, as a key cancer stem cell marker in tumor progression. In Gene, 827, 146448. doi:10.1016/j.gene.2022.146448. https://pubmed.ncbi.nlm.nih.gov/35337852/
3. Najafzadeh, Basira, Asadzadeh, Zahra, Motafakker Azad, Rohollah, Najafi, Souzan, Baradaran, Behzad. 2020. The oncogenic potential of NANOG: An important cancer induction mediator. In Journal of cellular physiology, 236, 2443-2458. doi:10.1002/jcp.30063. https://pubmed.ncbi.nlm.nih.gov/32960465/
4. Sun, Ai-Xi, Liu, Chang-Jiang, Sun, Zi-Qin, Wei, Zhi. . NANOG: a promising target for digestive malignant tumors. In World journal of gastroenterology, 20, 13071-8. doi:10.3748/wjg.v20.i36.13071. https://pubmed.ncbi.nlm.nih.gov/25278701/
5. Gong, Shuai, Li, Qiuhui, Jeter, Collene R, Tang, Dean G, Liu, Bigang. 2015. Regulation of NANOG in cancer cells. In Molecular carcinogenesis, 54, 679-87. doi:10.1002/mc.22340. https://pubmed.ncbi.nlm.nih.gov/26013997/
6. Yuan, Ye, Zhang, Mengsi, Yan, Guangning, Yang, Kaidi, Guo, Deyu. 2021. Nanog promotes stem-like traits of glioblastoma cells. In Frontiers in bioscience (Landmark edition), 26, 552-565. doi:. https://pubmed.ncbi.nlm.nih.gov/33049683/
7. Mahalaxmi, Iyer, Devi, Subramaniam Mohana, Kaavya, Jayaramayya, Balachandar, Vellingiri, Santhy, Kumaran Sivanandan. 2019. New insight into NANOG: A novel therapeutic target for ovarian cancer (OC). In European journal of pharmacology, 852, 51-57. doi:10.1016/j.ejphar.2019.03.003. https://pubmed.ncbi.nlm.nih.gov/30831081/
8. Fatma, Homa, Siddique, Hifzur R, Maurya, Santosh K. 2021. The multiple faces of NANOG in cancer: a therapeutic target to chemosensitize therapy-resistant cancers. In Epigenomics, 13, 1885-1900. doi:10.2217/epi-2021-0228. https://pubmed.ncbi.nlm.nih.gov/34693722/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen